Summary:The distributed brain systems associated with performance of a verbal fluency task were identified in a nondirected correlational analysis of neurophysiological data obtained with positron tomography. This analysis used a recursive principal-component analysis developed specifically for large data sets. This analysis is interpreted in terms of functional connectivity, defined as the tem poral correlation of a neurophysiological index measured in different brain areas. The results suggest that the vari ance in neurophysiological measurements, introduced exCooperative and connectionist approaches to un derstanding the integration of brain function are well established (Sherrington, 1941;Hebb, 1949;Edelman, 1978;McClelland, 1988). The nature and organizational principles of extrinsic cortical con nections, particularly the long corticocortical affer ents (e. g. , Goldman Rakic, 1988) has provided a basis for mechanistic descriptions of brain function (e. g. , Mesulam, 1990). These descriptions refer to parallel, massively distributed, and interconnected (sub)cortical areas. Anatomical connectivity is a necessary underpinning for these models and has been used to infer functional connectivity (e. g. , Zeki, 1990). This article describes one way of mea suring functional connectivity using positron emis sion tomographic (PET) measurements of neural ac tivity.PET is in a unique position to acquire data for this Received October 28, 1991; final revision rec::: ived May 8, 1992; accepted June 18, 1992. Address correspondence and reprint requests to Dr. K. 1.Friston at The Neurosciences Institute, 1230 York Ave., New York, NY 10021, U.S.A.Abbreviations used: ANCOV A, analysis of covariance; BA, Broadmann's area; DLPFC, dorsolateral prefrontal cortex; J-PSTH, Joint Peri Stimulus Time Histogram; PCA, principal component analysis; PET, positron emission tomography; rCBF, regional CBF. 5 peri mentally , was accounted for by two independent prin cipal components. The first, and considerably larger, highlighted an intentional brain system seen in previous studies of verbal fluency. The second identified a distrib uted brain system including the anterior cingulate and Wernicke's area that reflected monotonic time effects. We propose that this system has an attentional bias. Key Words: PET-Principal-component analysis-Functional connectivity-Effective connectivity-Verbal fluency Neural networks.sort of analysis because it samples the entire brain state in a uniform fashion. This allows all possible functional connections to be assessed using serial measurements of the same subject in different brain states. The shortcomings of PET include its rela tively poor spatiotemporal resolution and the exact nature of the dependency of measured regional CBF (rCBF) on neural discharge rates. However, PET can be used to address large scale functional connectivity as an important supplement to obser vations on the gross aspects of extrinsic anatomical connectivity and fine time scale effective connectiv ity defined by electrophysi...