Fractionation and identification of the phenolic compounds of Highbush blueberries (Vaccinium corymbosum, L.)

Kader, F.; Rovel, B.; Girardin, Michel; Metche, M.

doi:10.1016/0308-8146(95)00068-2

Cited by 131 publications

(93 citation statements)

References 16 publications

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“…b MS signal of peak 13 was overlapped and not fragmented in MS-MS. c Tentative identification data combined with literature (Kader et al, 1996). in different fruits (Table III).…”

Section: Discussionmentioning

confidence: 99%

“…With the more sensitive electrospray ionization mass spectrometry (ESI-MS) method, all 15 anthocyanins were detected (Table II). The identification was based on combining the ESI-MS data of anthocyanins with the well-known chromatographic profile of anthocyanins of bilberry (Kader et al, 1996). …”

Section: Expression Of the Flavonoid Pathway Genes In Color Mutantsmentioning

confidence: 99%

“…The major ion in MS ionization was selected to MS-MS fragmentation; therefore, one overlapped minor anthocyanin flavylium cation was not fragmented (Table II). The sugar moieties of anthocyanins found in bilberry were specified according to previous publications (Kader et al, 1996).…”

Section: Analysis Of Flavonoidsmentioning

confidence: 99%

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Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development

Määttä²,

et al. 2002

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The production of anthocyanins in fruit tissues is highly controlled at the developmental level. We have studied the expression of flavonoid biosynthesis genes during the development of bilberry (Vaccinium myrtillus) fruit in relation to the accumulation of anthocyanins, proanthocyanidins, and flavonols in wild berries and in color mutants of bilberry. The cDNA fragments of five genes from the flavonoid pathway, phenylalanine ammonia-lyase, chalcone synthase, flavanone 3-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase, were isolated from bilberry using the polymerase chain reaction technique, sequenced, and labeled with a digoxigenin-dUTP label. These homologous probes were used for determining the expression of the flavonoid pathway genes in bilberries. The contents of anthocyanins, proanthocyanidins, and flavonols in ripening bilberries were analyzed with high-performance liquid chromatography-diode array detector and were identified using a mass spectrometry interface. Our results demonstrate a correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of berries. At the early stages of berry development, procyanidins and quercetin were the major flavonoids, but the levels decreased dramatically during the progress of ripening. During the later stages of ripening, the content of anthocyanins increased strongly and they were the major flavonoids in the ripe berry. The expression of flavonoid pathway genes in the color mutants of bilberry was reduced. A connection between flavonol and anthocyanin synthesis in bilberry was detected in this study and also in previous data collected from flavonol and anthocyanin analyses from other fruits. In accordance with this, models for the connection between flavonol and anthocyanin syntheses in fruit tissues are presented.Fruit development from flower to ripe fruit is a complex process that involves modification of cellular compartments, loss of cell wall structure causing softening, and accumulation of carbohydrates (Brady, 1987). The production of secondary metabolites during the ripening process is an essential phenomenon for the contribution of seed dispersal of the plant in the form of accumulation of pigments and flavor compounds. The significance of secondary products in defense against diseases in developing fruits should also be remembered (Harborne, 1997;Mercier, 1997).Flavonoids are a large group of phenolic secondary metabolites that are widespread among plants and are involved in many plant functions. Anthocyanins, a flavonoid subclass, are the main pigments in flowers and fruits, acting as insect and animal attractants (Bohm, 1998;Harborne and Williams, 2000). Anthocyanins are synthesized via the phenylpropanoid pathway (Fig. 1). Anthocyanin biosynthesis has been extensively studied in several plant species, and, therefore, detailed information of the course of reactions is available. Two classes of genes are required for anthocyanin biosynthesis, the structural genes encoding the enzymes...

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“…b MS signal of peak 13 was overlapped and not fragmented in MS-MS. c Tentative identification data combined with literature (Kader et al, 1996). in different fruits (Table III).…”

Section: Discussionmentioning

confidence: 99%

Section: Expression Of the Flavonoid Pathway Genes In Color Mutantsmentioning

confidence: 99%

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Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development

Määttä²,

et al. 2002

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“…However, petunidin (5) and Malvidin (6), and their glycosides (30-35 and 36-41, respectively) are reported in subgenus Vaccinium only, some non glucoside and galactoside may restrict to specific species [24]. [69,77] V. angustifolium (fruit) [29] V. virgatum = V. ashei (fruit) [20] V. corymbosum (fruit) [18,46,95] V. covilleanum (fruit) [37] V. myrtillus (fruit) [21,43,45,46] V. membranaceum (fruit) [96] V. padifolium (fruit) [24] V. ovatum (fruit) [96] V. uliginosum (fruit) [33] V. vitis-idaea (fruit) [31,38,42] V. intermedium (fruit) [48] V. meridionale (fruit) [47] V. oxycoccus L. (fruit) [36] V. stamineum L. (fruit) [30] 10 Cyanidin-3-O-galactoside V. macrocarpon (fruit) [69,77] V. angustifolium (fruit) [29] V. virgatum = V. ashei (fruit) [20] V. corymbosum (fruit) [18,46,95] V. covilleanum (fruit) [37] V. myrtillus (fruit) [17,21] V. membranaceum (fruit) [96] V. padifolium (fruit) [24] V. ovatum (fruit) [96] V. uliginosum (fruit) [33] V. vitis-idaea (fruit) [31,39] V. intermedium (fruit) [22,…”

Section: Anthocyaninsmentioning

confidence: 99%

“…corymbosum (fruit) [18,46,95] V. covilleanum (fruit) [37] V. myrtillus (fruit) [17,21] V. membranaceum (fruit) [96] V. ovatum (fruit) [96] V. uliginosum (fruit) [33] V. vitis-idaea (fruit) [22,48,67] V. intermedium (fruit) [22,48] V. padifolium (fruit) [24] 27 Delphinidin-3-O-glucoside V. macrocarpon (fruit) [69,77] V. angustifolium (fruit) [29] V. virgatum = V. ashei (fruit) [20] V. corymbosum (fruit) [18,46,95] V. covilleanum (fruit) [37] V. myrtillus (fruit) [17,21] V. membranaceum (fruit) [96] V. arctostaphylos (fruit) [97] V. padifolium (fruit) [24] V. ovatum (fruit) [96] V. uliginosum (fruit) [33,100] V. vitis-idaea (fruit) [31] V. intermedium (fruit) [22,48] V. oxycoccus L. (fruit) [36] V. floribundum (fruit) [68] 28 …”

Section: Anthocyaninsmentioning

confidence: 99%

Anthocyanins and Flavonoids of Vaccinium L.

Su¹

2012

TOPHARMCJ

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Vaccinium L., comprising approximately 450 species primarily in the Northern Hemisphere, is a genus of shrubs or lianas in the family Ericaceae. The berries of many species are harvested for household consumption and commercial sale. The genus produces a wide range of compounds such as anthocyanins, flavonoids, chromones, coumarins, lignans, benzoic acids, iridoids, sterols, and triterpenoids, but is best known for the production of anthocyanins and flavonoids. Extracts and isolates of anthocyanins and flavonoids from Vaccinium fruits or leaves showed antioxidative, anti-inflammatory, antitumor, antiviral, vasoprotective, and antifungal activities. To data, more than 116 anthocyanins and flavonoids compounds have been isolated and identified primarily from the fruits or leaves of Vaccinium. This article reviews phytochemistry and pharmaceutical properties of these compounds.

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Mechanism of Browning in Fresh Highbush Blueberry Fruit (Vaccinium corymbosum L). Partial Purification and Characterisation of Blueberry Polyphenol Oxidase

Kader¹,

Rovel²,

Girardin³

et al. 1997

J. Sci. Food Agric.

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The most efficient method for polyphenol oxidase (PPO) extraction from Highbush blueberry fruits was the preparation of an acetone powder. No activity was detected after direct extraction with phosphate buffer (pH 6·5) and detergents such as Triton X‐100. PPO has been purified 19‐fold, by ultrafiltration, ammonium sulphate precipitation and hydrophobic chromatography. Native‐PAGE of the purified fraction revealed the presence of two isoforms. PPO has an observed optimum pH at 4·0, followed by a shoulder at pH 5·0. Caffeic acid is the best substrate (100%), followed by chlorogenic acid (60%) and pyrocatechol (32·5%). No activity was detected towards catechin, protocatechuic acid, resorcinol and monophenols. © 1997 SCI.

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Fractionation and identification of the phenolic compounds of Highbush blueberries (Vaccinium corymbosum, L.)

Cited by 131 publications

References 16 publications

Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development

Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development

Anthocyanins and Flavonoids of Vaccinium L.

Mechanism of Browning in Fresh Highbush Blueberry Fruit (Vaccinium corymbosum L). Partial Purification and Characterisation of Blueberry Polyphenol Oxidase

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