Fractionation and turnover of stable carbon isotopes in animal tissues: Implications for ?13C analysis of diet

Tieszen, Larry L.; Boutton, Thomas W.; Tesdahl, K. G.; Slade, Neda

doi:10.1007/bf00379558

Cited by 1,577 publications

(1,491 citation statements)

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“…Red blood cells and muscle were the tissues chosen for comparative analyses among the different species because these tissues integrated diet over relatively long periods (≥ 2 to 3 mo: Tieszen et al 1983, Hobson & Clark 1992b, Hilderbrand et al 1996.…”

Section: Study Area -Estuary and Gulf Of St Lawrencementioning

confidence: 99%

“…when δ 13 C values were corrected using a 1.3 ‰ 13 C-enrichment per trophic level ( Table 2). The δ 13 C value of Estuary harp seals suggests that they had been in the Estuary since at least early December, assuming a half-life of 4 wk for muscle tissue and a spread over 2 to 3 mo in δ 13 C changes (Tieszen et al 1983). This coincides with the seasonal timing of observations of large numbers of harp seals in the Lower Estuary (Sergeant 1991).…”

Section: Patterns Of 13 C Signatures and Trophic Relationshipsmentioning

confidence: 99%

“…blood plasma, liver) or a few months (e.g. red blood cells or muscle) (Tieszen et al 1983, Hobson & Clark 1992a, Hilderbrand et al 1996. This diet assessment method is relatively non-invasive.…”

Section: Introductionmentioning

confidence: 99%

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Marine mammals and the community structure of the Estuary and Gulf of St Lawrence, Canada: evidence from stable isotope analysis

Lesage¹,

Hammill²,

Kovacs³

2001

Mar. Ecol. Prog. Ser.

163

131

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The trophic relationships of both the benthic and pelagic communities in the Estuary and Gulf of St Lawrence regions were examined, with a special focus on the trophic position (TP) and relationship(s) among harbour, grey, hooded and harp seals and beluga whales. A multiple stable isotope and multiple tissue approach, used in conjunction with conventional dietary information, suggested that marine mammals occupied the highest trophic positions in the food webs of both communities and that they overlapped with one another to some extent trophically. Harbour seals Phoca vitulina and hooded seals Cystophora cristata occupied the highest TP, grey seals Halichoerus grypus, Gulf harp seals Phoca groenlandica, and male beluga whales Delphinapterus leucas were intermediate, and Estuary harp seals and female beluga whales were at the lowest TP. A general pattern of increasing enrichment of 13 C or 15 N with age was observed in marine mammals (as well as fishes), although yearlings showed a decreased enrichment compared to both younger and older age classes. Sex also influenced δ 15 N values. Males were more 15 N-enriched than females, with the difference between the sexes increasing with age, and being most pronounced in species that are sexually dimorphic with respect to body size. Geographical location also influenced isotope abundance. Estuary organisms were generally 13 C-enriched relative to Gulf animals. δ 13 C values were on average lower in short-term diet integrators (blood serum) than in longer-term diet integrators (red blood cells) of harbour seals captured in April to June in the Estuary, which suggests that they probably did not move outside the Lower Estuary during the winter. Grey seals captured in the Lower Estuary did, however, show evidence of having been in the Gulf region some weeks or months before capture.

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Section: Study Area -Estuary and Gulf Of St Lawrencementioning

confidence: 99%

Section: Patterns Of 13 C Signatures and Trophic Relationshipsmentioning

confidence: 99%

See 1 more Smart Citation

Marine mammals and the community structure of the Estuary and Gulf of St Lawrence, Canada: evidence from stable isotope analysis

Lesage¹,

Hammill²,

Kovacs³

2001

Mar. Ecol. Prog. Ser.

163

131

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“…It was also shown that the degree of this alteration depends on the component or body tissue considered, due to its biochemical composition and turnover rate. 22 On the other hand, variations in the SIRs in milk and milk derivatives following suitable experimental design have not been so extensively explored. Metges et al 23 investigated the 13 C/ 12 C of CO 2 breath, bulk milk and serum by exchanging a C 3 plant with a C 4 plant diet, and vice versa.…”

mentioning

confidence: 99%

Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Camin

Perini

Colombari

et al. 2008

Rapid Comm Mass Spectrometry

123

134

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The stable isotope ratios ( 13 C/ 12 C, 15 Recently, several papers have been published about the application of stable isotope ratio analysis (SIRA) using isotopic ratio mass spectrometry (IRMS) to animal products. The aims of these studies were to obtain dietary reconstruction, to study animal movement patterns, 1-5 and to characterise the geographical origin. [6][7][8][9][10][11][12][13][14][15][16] Another important aim could be to study and quantify the alteration of stable isotope ratios (SIRs) in milk by substituting a C 3 plant diet with maize. This could be important in order to protect milk and milk derivatives produced using traditional or 'organic' practices of pasture compared with products produced with cheaper diets based on maize derivatives. There have been recent reports about this in defatted meat, [17][18][19] in related fat, 17 in several animal tissues such as blood, plasma, liver, kidney, cow hair 20 and in sheep faeces. 21 In these papers it was shown that there is a strong correlation between the SIR of carbon and the amount of maize in the diet. It was also shown that the degree of this alteration depends on the component or body tissue considered, due to its biochemical composition and turnover rate. 22 On the other hand, variations in the SIRs in milk and milk derivatives following suitable experimental design have not been so extensively explored. Metges et al. 23 investigated the 13 C/ 12 C of CO 2 breath, bulk milk and serum by exchanging a C 3 plant with a C 4 plant diet, and vice versa. Wilson et al. 24 studied the contribution of body protein to milk protein, changing abruptly from a C 3 to a C 4 diet. More recently, Knobbe et al. 25 followed the variation in stable carbon and nitrogen isotopic composition under different feeding regimes. It was concluded that, to obtain more detailed information on the influencing factors, the analysis of the SIRs of other elements such as oxygen and hydrogen may be necessary.In this study we considered around 130 cows stabled in two farms and supplied with diets made up of different kinds of C 3 plants and different amounts of maize. The different feed ingredients, the overall diet and the relevant milk (casein, lipid and some bulk samples) were subjected to analysis of 13

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“…Stable isotope ratios of nitrogen ( 15 N/ 14 N, expressed as d 15 N) and carbon ( 13 C/ 12 C, expressed as d 13 C) in consumer tissues have been used to determine trophic relationships within food webs and whether a food chain or food web is based on C 3 plants (d 13 C & -23% to -32%), C 4 plants (d 13 C & -16% to -9%), or a mixture of the two groups (De Niro and Epstein 1978;Tieszen et al 1983). Similarly, stable isotopes of hydrogen in bird feathers (dD f ) have been positively correlated with stable isotopes of hydrogen in rain (dD p ; Chamberlain et al 1997;Hobson and Wassenaar 1997).…”

mentioning

confidence: 99%

Organochlorine Pollutants and Stable Isotopes in Resident and Migrant Passerine Birds from Northwest Michoacán, Mexico

Mora

2008

Arch Environ Contam Toxicol

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Although concentrations of organochlorine compounds (OCs) in birds from most of the United States and Canada have decreased over the last 30 years, there is still concern that migrant birds might be exposed to elevated concentrations of OCs during migration in Latin America. The Lerma-Chapala Basin in west-central Mexico is an important migration corridor and wintering area for many species. The objectives of this study were to assess if resident and migrant birds wintering in western Michoacán, Mexico accumulated elevated concentrations of OCs during fall and spring and to determine if the stable isotopes d 15 N, d 13 C, and dD could be used to predict burdens and origins of DDE accumulation. Resident and migrant passerine insectivorous birds were collected during fall and spring (2001)(2002) in northwest Michoacán, near Chapala Lake, Mexico. The carcasses were analyzed for OCs and tail feathers were analyzed for stable isotopes d 15 N, d 13 C, and dD. The OCs detected in more than 50% of the samples were: oxychlordane (79%), p,p'-DDE (100%), p,p 0 -DDT (57%), and total PCBs (100%). p,p 0 -DDE was the OC detected at the highest concentrations, whereas residues of other OCs were near or below detection limits. Overall, there were no significant differences in concentrations of OCs between seasons or between resident and migrant birds. Concentrations of DDE and oxychlordane were somewhat higher in migrant and resident birds during spring than in fall; however, concentrations were significantly different only for oxychlordane. Two resident birds collected in fall and spring had DDE residues [10 lg/g wet weight in carcass. There were no significant differences in d 13 C and d 15 N values among species, between seasons, or between migrant and resident birds. However, dD values were clearly different between species and helped differentiate migrant from resident birds. dD values also were negatively and significantly correlated with DDE concentrations in carcass. Birds with more depleted dD values in feathers tended to have higher DDE concentrations than those with less depleted dD values, suggesting a potential latitudinal accumulation of DDE. Overall, our results suggest that during fall and spring, there is not a significant buildup of persistent OCs in migrant and resident passerine insectivorous birds in northwest Michoacán, Mexico.

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Fractionation and turnover of stable carbon isotopes in animal tissues: Implications for ?13C analysis of diet

Cited by 1,577 publications

References 26 publications

Marine mammals and the community structure of the Estuary and Gulf of St Lawrence, Canada: evidence from stable isotope analysis

Marine mammals and the community structure of the Estuary and Gulf of St Lawrence, Canada: evidence from stable isotope analysis

Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk

Organochlorine Pollutants and Stable Isotopes in Resident and Migrant Passerine Birds from Northwest Michoacán, Mexico

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