Fractionation of Protein Hydrolysates of Fish Waste Using Membrane Ultrafiltration: Investigation of Antibacterial and Antioxidant Activities

Pezeshk, Samaneh; Ojagh, Seýed Mahdi; Rezaei, Masoud; Shábanpour, Bahareh

doi:10.1007/s12602-018-9483-y

Cited by 92 publications

(52 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results were comparable to the values obtained in two previous works against S. aureus bacteria; the first paper was carried out on yellowfin tuna viscera bioactive peptide hydrolyzed fractions, where the MIC value was 0.5 mg mL −1 [ 35 ]; in the second one, the antimicrobial activity of the fractions isolated from bromelain hydrolysate of leatherjacket ( Meuchenia sp.) insoluble muscle proteins showed a MIC value of 4.3 mg mL −1 [ 36 ].…”

Section: Resultssupporting

confidence: 87%

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Cerrato

Capriotti

Capuano

et al. 2020

Foods

View full text Add to dashboard Cite

Due to the rapidly increasing resistance to conventional antibiotics, antimicrobial peptides are emerging as promising novel drug candidates. In this study, peptide fragments were obtained from yellowfin tuna muscle by simulated gastrointestinal digestion, and their antimicrobial activity towards Gram-positive and Gram-negative bacteria was investigated. In particular, the antimicrobial activity of both medium- and short-sized peptides was investigated by using two dedicated approaches. Medium-sized peptides were purified by solid phase extraction on C18, while short peptides were purified thanks to a graphitized carbon black sorbent. For medium-sized peptide characterization, a peptidomic strategy based on shotgun proteomics analysis was employed, and identification was achieved by matching protein sequence database by homology, as yellowfin tuna is a non-model organism, leading to the identification of 403 peptides. As for short peptide sequences, an untargeted suspect screening approach was carried out by means of an inclusion list presenting the exact mass to charge ratios (m/z) values for all di-, tri- and tetrapeptides. In total, 572 short sequences were identified thanks to a customized workflow dedicated to short peptide analysis implemented on Compound Discoverer software.

show abstract

Section: Resultssupporting

confidence: 87%

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Cerrato

Capriotti

Capuano

et al. 2020

Foods

View full text Add to dashboard Cite

show abstract

“…Hydrolysation unfolds complex protein structure to produce low molecular weight peptides and amino acids, which improve antioxidant activity of hydrolysed protein in comparison with the intact protein (Sarmadi & Ismail 2010). FPH from yellowfin tuna Thunnus albacores, tilapia Oreochromis niloticus, shortfn scad Decapterus macrosoma, salmon Salmo salar and cod Gadus morhua have been reported to possess antioxidant properties (Farvin et al 2014;Borawska et al, 2016;Tejpal et al 2017;Kang et al 2018;Pezeshk et al 2019). A study on red sea bream Pagrus major demonstrated an increase in superoxide dismutase in fish fed protein hydrolysate diets compared to the low FM-based diet; in addition, comparatively higher glutathione peroxidase activity (GPx) was observed in fish fed high FM-based diet and hydrolysate diets when compared to low FM-based diet (Khosravi et al 2015b).…”

Section: Injection 4 Weeksmentioning

confidence: 99%

“…2018; Pezeshk et al . 2019). A study on red sea bream Pagrus major demonstrated an increase in superoxide dismutase in fish fed protein hydrolysate diets compared to the low FM‐based diet; in addition, comparatively higher glutathione peroxidase activity (GPx) was observed in fish fed high FM‐based diet and hydrolysate diets when compared to low FM‐based diet (Khosravi et al .…”

Section: Biochemical Responsesmentioning

confidence: 99%

Enzymatic fish protein hydrolysates in finfish aquaculture: a review

Siddik

Howieson

Fotedar

et al. 2020

Reviews in Aquaculture

132

View full text Add to dashboard Cite

In intensive farming systems, fish are held at high densities, which may increase stress, leading to susceptibility to diseases that result in economic losses. Therefore, effective feeding practices incorporating health-promoting compounds such as proteins, hydrolysates and bioactive peptides that can stimulate the defence mechanisms of fish and achieve better growth are some of the priorities for sustainable aquaculture development. Globally, the fish processing industries generate and discard a large volume of waste every year, estimated at up to 60% of the harvested biomass. This waste can be converted to value-added products such as fish protein hydrolysate (FPH) with the addition of various proteolytic enzymes. FPH from fish processing waste including skin, heads, muscle, viscera, liver and bones is a good source of protein, amino acids, peptides and antioxidants and has been found to possess desirable functional and bioactive peptides. A moderate inclusion of FPH in aquafeeds has the potential to improve growth, feed utilization, immune functions and disease resistance of fish. Production of FPH, targeted to more precise molecular weight ranges, has superior functionalities that are in high demand. With interest in FPH as an aquafeed supplement, this review aimed to summarize the source, production processes and functional properties of FPH and the reported impact of FPH in aquafeed supplement on fish growth, survival, feed utilization, immune response and disease resistance. Possible limitations of using FPH and future research potential as an opportunity for the use of processing fish waste are also discussed.

show abstract

“…Contudo, a maior parte dos trabalhos com hidrolisados proteicos de peixes descreve os efeitos dos hidrolisado na nutrição animal, como os realizados por Rimoldi et al (2020) em que os autores estudaram os efeitos da proteína hidrolisada de peixe e da levedura autolisada como substitutos da farinha de peixe na dieta de dourada (Sparus aurata), na microbiota intestinal de peixes. Pezeshk et al (2019) utilizaram o fracionamento hidrolisados proteicos de resíduos de atum albacora (Thunnus albacores) usando ultrafiltração de membrana (tamanho molecular: <3, 3-10, 10-30 e 30 kDa <) e puderam constatar as propriedades antibacterianas e antioxidantes a partir da fração de menor peso molecular (<3 kDa) teve significativamente a maior (P <0,05) porcentagem de inibição de bactérias contra Gram-positivos (Listeria e…”

Section: Hidrolisado Proteico De Peixeunclassified

Possibilidades Proteicas Do Processamento Agroindustrial De Peixes

Silva¹,

Nascimento²,

Costa³

et al. 2021

Inovação, Gestão E Sustentabilidade Na Agroindústria

View full text Add to dashboard Cite

RESUMOO peixe tem se tornado cada vez mais hábito na alimentação humana, visando, sobretudo, o bem Fonte rica de compostos biologicamente favoráveis, uma alimentação com peixes também tem a seu favor os custos e a palatabilidade do filé. O entrave proveni resíduos sólidos concomitantes ao processamento dos peixes, onde são descartadas as partes de baixo valor comercial ou aproveitamento biotecnológico, como cabeças, ossos, carcaças, pele, escamas e vísceras internas, como estômago, intestinos, cecos pilóricos, fígados, resquícios de músculos, bexiga natatória, entre outros, fontes comprovadamente de proteínas de alto valor biológico. trabalho objetivou revisar sobre as principais possibilidades proteicas de proteínas musculares, moléculas de colágeno, gelatinas, enzimas e os hidrolisados proteicos produzidos com partes ou carcaças de peixes. como: ScienceDirect, Scielo, Elsevier descritores de busca os seguintes termos: "proteínas de peixes", "colágeno de peixes", "gelatina de peixe", "enzimas de peixe", "hidrolisado proteico de peixes". As inúmeras possibilidades de aproveitamento das proteínas provenientes do processamento de peixes têm atraído cada vez mais os segmentos industriais voltados para alimentação e produção de compostos nutracêuticos. Com esta revisão, foi possível evidenciar as possibilidades de proteínas do filé, escamas, bexiga natatória e ossos, da gelatina produzida pela hidrólise parcial das moléculas dos subtipos de colágeno, pelas enzimas, especialmente as digestivas, pela produção de hidrolisados proteicos e, por fim, a sinaliza crescente por produtos provenientes da Pesca e Aquicultura para fins biotecnológicos.Palavras-Chave: proteínas, pescados, piscicultura, biotecnologia. RESUMEN El pescado se ha convertido cada vez más en un hábito en la alimentación humana, apuntando, sobre todo, al bienestar. Una rica fuente de compuestos biológicamente favorables, una dieta con pescado también tiene los costos y la palatabilidad del filete a su

show abstract

Fractionation of Protein Hydrolysates of Fish Waste Using Membrane Ultrafiltration: Investigation of Antibacterial and Antioxidant Activities

Cited by 92 publications

References 54 publications

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Identification and Antimicrobial Activity of Medium-Sized and Short Peptides from Yellowfin Tuna (Thunnus albacares) Simulated Gastrointestinal Digestion

Enzymatic fish protein hydrolysates in finfish aquaculture: a review

Possibilidades Proteicas Do Processamento Agroindustrial De Peixes

Contact Info

Product

Resources

About