Fractionation of red wine polyphenols by solid-phase extraction and liquid chromatography

Sun, Baoshan; Leandro, M. Conceição; Freitas, Víctor de; Spranger, M. Isabel

doi:10.1016/j.chroma.2006.06.026

Cited by 88 publications

(62 citation statements)

References 34 publications

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“…Separation was patterned after methods developed for wine polyphenolics (Sun et al, 2006). A C18 PrepSep column (Fisher Scientific 11-131-11 5 g / 20 ml) was conditioned with 200 ml methanol followed by 200 ml water at pH 7.…”

Section: Fractionation Methodsmentioning

confidence: 99%

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

“…Fraction A eluted with water as a bright yellow liquid believed to contain phenolic acids and hydrolyzable tannins according to prior work on polyphenolic separation (Oszmianski et al, 1988;Sun et al, 2006). Fraction B, which eluted with ethyl acetate, was colorless to pale yellow and likely contained colorless proanthocyanins, flavanols, and some monomer and oligomer phenolic acids (He et al, 2006;Oszmianski et al, 1988;Sun et al, 2006). Fraction C, which eluted with acidified methanol, was dark brown indicating the presence of complex tannins, pigmented proanthocyanidins, and pyranoanthocyanins (He et al, 2006;Oszmianski et al, 1988;Sun et al, 2006).…”

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

“…We adopted a method based on the resolution of wine polyphenols (Sun et al, 2006) . A schematic of fractionation can be seen in Figure 5.2.…”

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

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Anti-Quorum Sensing Agents from South Florida Medicinal Plants and their Attenuation of Pseudomonas Aeruginosa Pathogenicity

Adonizio¹

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This dissertation, written by Allison L. Adonizio, and entitled Anti-quorum sensing agents from south Florida medicinal plants and their attenuation of Pseudomonas aeruginosa pathogenicity, having been approved in respect to style and intellectual content, is referred to you for judgment.We have read this dissertation and recommend that it be approved. Each plant presented a distinct profile of effect on QS genes and signaling molecules, suggesting varying modes of action. Virulence attenuation was observed with marginal reduction of bacterial growth, suggesting quorum quenching mechanisms unrelated to static or cidal effects. Extracts of these plants were also investigated for their effects on P. aeruginosa killing of the nematode Caenorhabditis elegans. Results were evaluated in both toxin-based and infection-based assays with P. aeruginosa strains PA01 and PA14. _____________________________Overall nematode mortality was reduced 50-90%. There was no indication of host toxicity, suggesting the potential for further development as anti-infectives. Using lowpressure chromatography and HPLC, two stereoisomeric ellagitannins, vescalagin and castalagin were isolated from an aqueous extract of C. erectus. Structures were confirmed via mass spectrometry and NMR spectroscopy. Both ellagitannins were shown to decrease signal production, QS gene expression, and virulence factor production in P.aeruginosa. This study introduces a potentially new therapeutic direction for the treatment of bacterial infections. In addition, this is the first report of vescalagin and castalagin being isolated from C. erectus, and the first report of ellagitannin activity on the QS system.

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Section: Fractionation Methodsmentioning

confidence: 99%

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

“…We adopted a method based on the resolution of wine polyphenols (Sun et al, 2006) . A schematic of fractionation can be seen in Figure 5.2.…”

Section: Fractionation Of Polyphenolics In Crude Extractmentioning

confidence: 99%

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Anti-Quorum Sensing Agents from South Florida Medicinal Plants and their Attenuation of Pseudomonas Aeruginosa Pathogenicity

Adonizio¹

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“…The Savuto is mainly produced from gaglioppo grape (Vitis vinifera L., cv) and is selected because it is one of the best varieties produced in Italy. The stem contact during grape skin in Fermentation provides many monomer and polymer proanthocyanidins [11][12][13] to the wine. The procedure of Fermentation and its post-treatment for bottling were carried out as described earlier.…”

Section: Preparation Of Red Winementioning

confidence: 99%

In vitro action of 2.2’-azobis-2 amidinopropan dihydrochloride,red wine polyphenols, resveratrol and catechin on anion permeability for chloride in human red blood cell

Gallo

Martino

2014

Free Rad. Antiox.

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Introduction:The purpose of this study was to evaluate the ability of polyphenols (red wine polyphenols, resveratrol and catechin) on anion permeability for chloride and oxidative damage in human erythrocytes exposed to the water-soluble free radical initiator 2.2'-azobis-2 amidinopropan dihydrochloride (AAPH). Materials and Methods: The reducing agent treatment efficacy was observed by evaluation of anion permeability for chloride, lipid peroxidation and hemolysis in red blood cells (RBCs). Anion permeability for chloride is an indicator of membrane protein damage and is evaluated in RBCs by the specific absorption of methemoglobin (C M ) at 590 and 635 nm after treatment of heparinized blood with NaNO 2 . The measurement of the membrane lipid degradation is obtained by the determination of malondialdehyde. The lipid peroxidation susceptibility is observed after the oxidative stress induced by AAPH. The hemolysis assays are conducted on blood samples in phosphate buffer saline and the morphological changes of erythrocytes are observed by optical microscopy as membrane damage verification parameter. Results: Polyphenol treatment is associated with a significant increase in anion permeability for chloride compared with control and AAPH affected cells. Treatment with polyphenols was associated with a significant reduction in mean ± standard error of the mean membrane lipid peroxidation compared with control and AAPH treatment. Hemolysis data are also obtained in the previously described conditions. RBCs morphology data indeed confirm previous observations. Conclusion: In this study, it is evident that after in vitro oxidative damage of the membrane, red wine polyphenol extracts are as effective reducing agents also on C M indicator of membrane protein damages. Consequently, both red wine and polyphenol extracts both alone and mixed among them efficiently relieve the effects of oxidative stress.

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Grape and Wine Polyphenols

Flamini

Traldi

2009

Mass Spectrometry in Grape and Wine Chemistry

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Fractionation of red wine polyphenols by solid-phase extraction and liquid chromatography

Cited by 88 publications

References 34 publications

Anti-Quorum Sensing Agents from South Florida Medicinal Plants and their Attenuation of Pseudomonas Aeruginosa Pathogenicity

Anti-Quorum Sensing Agents from South Florida Medicinal Plants and their Attenuation of Pseudomonas Aeruginosa Pathogenicity

In vitro action of 2.2’-azobis-2 amidinopropan dihydrochloride,red wine polyphenols, resveratrol and catechin on anion permeability for chloride in human red blood cell

Grape and Wine Polyphenols

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