Fractionation of Sera Containing Antibodies against Red Cells or Platelets with Special Reference to Anti‐D Sera

Summary In a study of 24 red cell eluates obtained from patients with acquired haemolytic anaemia of the ‘warm’ type, it was established that some eluates possessed autoantibody of only one specificity either for ‘normal’ (‘anti‐nl’) or ‘Rhnull’ (‘anti‐dl’) red cells, whereas others contained autoantibodies of both specificities. Combined autoantibodies were often better recognized by different testing procedures than by the same method. Thus the ability of these autoantibodies to strongly sensitize ‘normal’ red cells by the ficin test and ‘Rhnull’ cells by the antiglobulin test was frequently found, whereas the occurrence of strong ficin‐reactive ‘anti‐nl’ and strong ficin‐reactive ‘anti‐dl’ was rare. Additional studies using red cell eluates revealed that no direct correlation could be established between the presence of complement components and the intensity of the indirect antiglobulin test using anti‐IgG serum. On the other hand, the simultaneous presence of ‘anti‐nl’ and ‘anti‐dl’ autoantibodies was often associated with the presence of complement components in the red cell eluates. Fixation of complements by Rh isoantibodies of multiple specificity has been reported, and thus the presence of complement components in association with ‘anti‐nl’ and ‘anti‐dl’ may be analogous to complement fixation by multiple Rh isoantibodies. The formation of ‘anti‐nl’ with ‘anti‐dl’ which tends to result in complement components being fixed by the antibody may be considered as a natural progression of antibody synthesis associated with an advanced state of autoimmunization.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Fractionation of Sera Containing Antibodies against Red Cells or Platelets with Special Reference to Anti‐D Sera

Cited by 6 publications

References 1 publication

Anti-K2 (Cellano) blood group antibodies

Anti-K2 (Cellano) blood group antibodies

A New Precipitating Antigen-Antibody System (Pennsylvania Antigen) in Human Serum

Specificity of Acquired Haemolytic Anaemia Autoantibodies and their Scrological Characteristics

Contact Info

Product

Resources

About