This study aimed to conduct a mixed-methods SR of the literature investigating the experiences (knowledge, attitudes, practices, and effects of use) concerning dental dam and cling film use. The SR methodology was informed by the Preferred Reporting Items for SRs and Meta-analysis framework, the Cochrane Handbook for SR of Interventions, the Joanna Briggs Institute guideline for conducting mixed-methods SRs, and the PICO (population, intervention (or exposure), comparison, and outcome) framework. Using a set of inclusion criteria, relevant studies in the literature were obtained for the review from 11 electronic databases, Teesside University Library, websites of multilateral health organisations, and reference lists of eligible literature. The included literature was appraised for quality using the Mixed Methods Appraisal Tool version 2018 and the AAOCDS Tool. Only those studies in the literature with sufficient quality were finally included for data extraction (using an adapted version of the JBI Quality Assessment and Review Instruments) and synthesis. Best evidence synthesis was performed for the quantitative data, while meta-aggregation was performed for the qualitative data. The synthesised data were then configured using the segregated approach. A total of 529 studies from the literature were obtained from the searched databases, while only four were obtained from the other sources. After de-duplication, screening, and quality appraisal, a total of 17 studies were found eligible and included in this review. Sixteen studies were non-grey literature while, one was grey literature, and only a few reported a finding on cling film and heterosexual populations. A total of 5516 adolescents and adults were investigated in the analysed literature. Overall, the reported knowledge of dental dams was generally poor, while no study reported knowledge of cling film. The attitudes towards dental dams/cling film were complex and predominantly negative, with very few participants using dental dams/cling film for oral sex. No study reported any finding on the effect(s) of dental dam/cling film use. In conclusion, there is a need for robust and strategic public health interventions for sexual health and safer oral sex practices.