Free 4-Hydroxyproline Content in Serum of Bedridden Aged People Is Elevated Due to Fracture.

Koike, Katsuya; Li, Yanmei; Seo, Miyuki; Sakurada, Ikuo; Tezuka, Ko; Uchikawa, K

doi:10.1248/bpb.23.101

Cited by 17 publications

(8 citation statements)

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“…The same is true for threonine, a major component of proteins, e.g., of collagen and immunoglobulins: We found decreased serum threonine levels, and decreased plasma levels are described in patients with chronic heart failure 17 . The increase that we observed in hydroxyproline, an analyte used to track collagen degradation, also reflects elevated protein turnover: Elevated urine and serum hydroxyproline levels are reported after muscle damage or in the bedridden and elderly 19,20 . AAA, aromatic amino acids; Ac-Orn, acetylornithine; ADMA, asymmetric dimethylarginine; alpha-AAA, alpha aminoadipic acid; BCAA, branched-chain amino acids; c4-OH-Pro, cis-4-hydroxyproline; DOPA, dihydroxyphenylalanine; Met-SO, methionine sulfoxide; Nitro-Tyr, nitrotyrosine; PEA, phenylethylamine; SDMA, symmetric dimethylarginine; t4-OH-Pro, trans-4-hydroxyproline; ↑, statistically significant higher serum concentration in Fontan patients than in controls; ↓, statistically significant lower serum concentration in Fontan patients than in controls.…”

Section: Discussionmentioning

confidence: 74%

Targeted metabolomic analysis of serum amino acids in the adult Fontan patient with a dominant left ventricle

Michel

Dubowy

Entenmann

et al. 2020

Sci Rep

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Growing interest lies in the assessment of the metabolic status of patients with a univentricular circulation after Fontan operation, especially in changes of amino acid metabolism. Using targeted metabolomic examinations, we investigated amino acid metabolism in a homogeneous adult Fontanpatient group with a dominant left ventricle, seeking biomarker patterns that might permit better understanding of fontan pathophysiology and early detection of subtle ventricular or circulatory dysfunction. We compared serum amino acid levels (42 analytes; AbsoluteIDQ p180 kit, Biocrates Life Sciences, Innsbruck, Austria) in 20 adult Fontan patients with a dominant left ventricle and those in age-and sex-matched biventricular controls. Serum concentrations of asymmetric dimethylarginine, methionine sulfoxide, glutamic acid, and trans-4-hydroxyproline and the methionine sulfoxide/ methionine ratio (Met-SO/Met) were significantly higher and serum concentrations of asparagine, histidine, taurine, and threonine were significantly lower in patients than in controls. Met-SO/ Met values exhibited a significant negative correlation with oxygen uptake during exercise. The alterations in amino acid metabolome that we found in fontan patients suggest links between fontan pathophysiology, altered cell energy metabolism, oxidative stress, and endothelial dysfunction like those found in biventricular patients with congestive heart failure. Studies of extended amino acid metabolism may allow better understanding of fontan pathophysiology that will permit early detection of subtle ventricular or circulatory dysfunction in Fontan patients. Ventricular dysfunction and circulatory failure with progressing end-organ impairment like renal or liver dysfunction are an important cause of morbidity and mortality in adults with complex congenital heart disease (CHD), especially in patients with single-ventricle types of CHD and Fontan circulation 1,2. Besides limited cardiac output, alterations that mark Fontan hemodynamics are passive flow to the lungs, chronically elevated venous pressures, and congestion. Unfortunately, the clinical use of traditional markers such as N-terminal prohormone of brain natriuretic peptide (NT-proBNP) levels for non-invasive diagnostics and monitoring in such patients is limited 3,4. Thus, for early detection of cardiac and circulatory derangement and for evaluation and tailoring of treatment options, regular functional assessment of these patients is crucial, with complete clinical examination, electrocardiogram, imaging studies, determination of values for traditional laboratory markers, or exercise capacity testing.

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Section: Discussionmentioning

confidence: 74%

Targeted metabolomic analysis of serum amino acids in the adult Fontan patient with a dominant left ventricle

Michel

Dubowy

Entenmann

et al. 2020

Sci Rep

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“…Pro, Hyp, and other small prolyl‐peptides are present in urine as degradation products of collagen, while Hyp is additionally reported as a marker of collagen catabolism . In the collagen helix, the repetitive triplet Gly‐X‐Y frequently occurs in the form of Gly‐Pro‐Hyp due to a high stability provided by the imino acids.…”

Section: Discussionmentioning

confidence: 99%

Combined Markers to Assess Meat Intake—Human Metabolomic Studies of Discovery and Validation

Cuparencu

Rinnan

Dragsted

2019

Molecular Nutrition Food Res

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Scope Biomarkers of red meat may clarify the relationship between meat intake and health. This paper explores the discovery of biomarkers of intake for three types of meat with varying heme iron content. Candidate biomarkers for red and general meat are further evaluated based on defined validation criteria. Methods and results In a randomized cross‐over meal study, healthy volunteers consume a randomized sequence of four test meals: chicken, pork, beef, and a control made of egg white and pea. Fasting and postprandial urine samples are collected to cover 48 h and profiled by untargeted LC‐ESI‐qTOF‐MS metabolomics. The profiles following the meal challenges are explored by univariate and multivariate analyses. Nine red, four white, and eight general meat biomarkers are selected as putative biomarkers, originating from collagen degradation, flavour compounds, and amino acid metabolism. Heme‐related metabolites are masked by the chlorophyll content of the control meal. The candidate biomarkers are confirmed in an independent meal study and validated for plausibility, robustness, time‐response, and prediction performance. Combinations of biomarkers are more efficient than single markers in predicting meat intake. Conclusion New combinations of partially validated biomarkers are proposed to assess terrestrial meat intake and thus help disentangle the effects of meat consumption on human health.

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“…Plasma 4-hydroxyproline concentrations were still different between the beef and fish meals at 7 h postmeal, suggesting that 4-hydroxyproline could have the potential to be a marker of beef intake in fasting plasma samples. Plasma 4-hydroxyproline is also proposed as a marker of collagen turnover in tissue, being elevated in elderly and bedridden subjects (37), so high intake of beef should be considered to be a confounding factor when using 4-hydroxyproline as a marker of collagen turnover. Although several potential biomarkers for meat and fish intakes have been found in urine (38), there is a lack of validated plasma biomarkers for meat and fish intake beyond n-3 FAs for fish.…”

Section: Discussionmentioning

confidence: 99%

Herring and Beef Meals Lead to Differences in Plasma 2-Aminoadipic Acid, β-Alanine, 4-Hydroxyproline, Cetoleic Acid, and Docosahexaenoic Acid Concentrations in Overweight Men

Ross

Svelander

Undeland

et al. 2015

The Journal of Nutrition

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These results in overweight men confirm that DHA and cetoleic acid reflect herring intake, whereas β-alanine and 4-hydroxyproline are potential biomarkers for beef intake. The greater postprandial rise in 2-aminoadipic acid after the beef meal, coupled to its proposed role in stimulating insulin secretion, may have importance in the context of red meat intake and increased diabetes risk. This trial was registered at clinicaltrials.gov as NCT02381613.

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Free 4-Hydroxyproline Content in Serum of Bedridden Aged People Is Elevated Due to Fracture.

Cited by 17 publications

References 0 publications

Targeted metabolomic analysis of serum amino acids in the adult Fontan patient with a dominant left ventricle

Targeted metabolomic analysis of serum amino acids in the adult Fontan patient with a dominant left ventricle

Combined Markers to Assess Meat Intake—Human Metabolomic Studies of Discovery and Validation

Herring and Beef Meals Lead to Differences in Plasma 2-Aminoadipic Acid, β-Alanine, 4-Hydroxyproline, Cetoleic Acid, and Docosahexaenoic Acid Concentrations in Overweight Men

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