Free calcium wave upon activation in Xenopus eggs

Kubota, Hiroshi; Yoshimoto, Yasuaki; Yoneda, Masafumi; Hiramoto, Yukio

doi:10.1016/0012-1606(87)90214-4

Cited by 146 publications

(57 citation statements)

References 26 publications

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“…Fertilization of the vertebrate egg causes a wave of calcium ions to spread throughout the cell from the site of sperm entry (Kubota et al 1987). The resultant rapid depolarization of the plasma membrane constitutes the primary block to polyspermy (Wyrick et al 1974;Wolf et al 1976).…”

Section: + Release and Mos Regulationmentioning

confidence: 99%

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Tunquist

Maller²

2003

Genes Dev.

229

187

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Section: + Release and Mos Regulationmentioning

confidence: 99%

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Tunquist

Maller²

2003

Genes Dev.

229

187

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“…In large eggs such as medaka (2,18) and Xenopus (9) eggs, a ring-shaped wave of [Ca2+] increase propagated through the egg from the point of sperm entry or pricking. In smaller cells such as sea urchin and golden hamster eggs (1,3,12), the increase also travels across the entire cell.…”

mentioning

confidence: 99%

Simultaneous investigation of intracellular Ca2+ increase and morphological events upon fertilization in the sand dollar egg.

Hamaguchi

1990

Cell Struct. Funct.

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ABSTRACT. An increase in intracellular Ca2+ concentration ([Ca2+]) and morphological events were simultaneously observed by epifluorescence and differential interference contrast (DIC) microscopy during fertilization of the sand dollar, Clypeasterjaponicus.[Ca2+], which was detected by a Ca2+ indicator, Fluo-3, initially increased just beneath the sperm-attached site on the egg surface 8.6 sec after attachment. The increase spread into the egg as a concentric sphere to the egg center and, thereafter, propagated in the egg cytoplasm as a planar wave rather than a spherical wave. It reached the site opposite the initiation site across the egg 24.2 sec after initiation. were prepared as described as earlier (5) and were also injected. The experiment was carried out at 25± 1°C. Fluo-3-injected eggs were observed with a Nikon microscope equipped with a simultaneously observable system of both DIC and epifluorescence using a B-2 filter cassette. This microscope is modified in three points compared to the convenient microscope which is used for DIC or epifluorescence by changing optical setup alternatively; the analyzer is set after the barrier filter in the optical path in order that the fluorescence is not reduced by the analyzer, the azimuth angle between the dichroic mirror and the analyzer or the polarizer is 90°instead of 45°, and single objectives can be utilized for both DIC and epifluorescence observation. In order to distinguish the fluorescent image from the DIC image, the latter was formed by red transmitted light with wavelength greater than 580 nm which was obtained by adding a longpass barrier filter to the illuminating system, whereas the former was green (the peak wavelength of fluorescence emission of Fluo-3 is 526nm (ll)). Therefore, structures in the DICimage such as sperm, egg cytoplasm, and FE were observed in red color, whereas the [Ca2+]-increased area in the egg became yellow as a result of mixing red and green light. Fluorescence and DICimages were accumulated for 1.5 sec on 3 CCDelements of a CCDcolor camera (HCC-3600, Flovel Co. Ltd., Tokyo) whose analog RGBsignal was monitored on a TVwhose screen was photographed at 1.5 sec intervals. The microscopic images were recorded through the video camera 159

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“…At fertilization, eggs from a variety of species show a wave-like propagating Ca2+ increase supported by internal stores (28,11,12,5,23,27, reviewed in 18 and 19). Such an increase, physiologically triggered by the sperm, functions as a second messenger which mediates many early and late activities of the fertilizing egg (11,21).…”

Section: Introductionmentioning

confidence: 99%

Cytochemical Ca²⁺ Distribution in Activated Discoglossus pictus Eggs: A Gradient in the Predetermined Site of Fertilization

Gualtieri¹,

Campanella²,

Andreuccetti³

1992

Dev Growth Differ

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The K-pyroantimonate/Os04 (PA) cytochemical method coupled with EGTA and X-ray microanalytical controls has been used to localize Ca2+ at egg activation in Discoglossus pictus eggs. The results show that: 1) the PA method is able to selectively localize Ca2+ pools mobilized by activating stimuli; 2) the smooth endoplasmic reticulum (SER) elements located in the animal dimple region, i.e. in the predetermined site of fertilization, are the first egg components labeled by precipitates; 3) a decreasing gradient of precipitates is present from the center beyond the boundaries of the dimple region; 4) precipitates are lacking in the remainder of the egg even at late times after activation.The possibilities are discussed that a) SER is the major Ca2+-releasing store at activation in Discoglossus, and b) the observed gradient of pyroantimonate-detected Ca2+ reflects an ionic Ca2+ gradient.

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Free calcium wave upon activation in Xenopus eggs

Cited by 146 publications

References 26 publications

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Simultaneous investigation of intracellular Ca2+ increase and morphological events upon fertilization in the sand dollar egg.

Cytochemical Ca²⁺ Distribution in Activated Discoglossus pictus Eggs: A Gradient in the Predetermined Site of Fertilization

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Free calcium wave upon activation in Xenopus eggs

Cited by 146 publications

References 26 publications

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs

Simultaneous investigation of intracellular Ca2+ increase and morphological events upon fertilization in the sand dollar egg.

Cytochemical Ca2+ Distribution in Activated Discoglossus pictus Eggs: A Gradient in the Predetermined Site of Fertilization

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Cytochemical Ca²⁺ Distribution in Activated Discoglossus pictus Eggs: A Gradient in the Predetermined Site of Fertilization