Free-Living Heterotrophic Nitrogen-Fixing Bacteria Isolated from Fuel-Contaminated Antarctic Soils

Eckford, Ruth; Cook, F. D.; Saul, David; Aislabie, Jackie; Foght, Julia M.

doi:10.1128/aem.68.10.5181-5185.2002

Cited by 90 publications

(51 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the fact that those organisms grew also on individual pure aliphatic and aromatic hydrocarbons confirms and consolidates their true diazotrophy. In the literature, there are some earlier reports on such bacteria in soil (Perez-Vargas et al 2000;Eckford et al 2002;Prantera et al 2002;Sorkhoh et al 2010b;Dashti et al 2009;Al-Mailem et al 2010). It is selfexplanatory that all these localities, like all others in nature, harbor, in addition, classic, diazotrophic, nonhydrocarbonutilizing bacteria.…”

Section: Discussionmentioning

confidence: 91%

Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

et al. 2012

View full text Add to dashboard Cite

Kuwaiti habitats with two-decade history of oil pollution were surveyed for their inhabitant oil-utilizing bacterioflora. Seawater samples from six sites along the Kuwaiti coasts of the Arabian Gulf and desert soil samples collected from seven sites all over the country harbored oil-utilizing bacteria whose numbers made up 0.0001-0.01% of the total, direct, microscopic counts. The indigenous bacterioflora in various sites were affiliated to many species. This was true when counting was made on nitrogen-containing and nitrogen-free media. Seawater samples harbored species belonging predominantly to the Gammaproteobacteria and desert soil samples contained predominantly Actinobacteria. Bacterial species that grew on the nitrogen-free medium and that represented a considerable proportion of the total in all individual bacterial consortia were diazotrophic. They gave positive acetylene-reduction test and possessed the nifH genes in their genomes. Individual representative species could utilize a wide range of aliphatic and aromatic hydrocarbons, as sole sources of carbon and energy. Quantitative determination showed that the individual species consumed crude oil, n-octadecane and phenanthrene, in batch cultures. It was concluded that the indigenous microflora could be involved in bioremediation programs without bioaugmentation or nitrogen fertilization. Irrigation would be the most important practice in bioremediation of the polluted soil desert areas.

show abstract

Section: Discussionmentioning

confidence: 91%

Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Temperature is one of the most important environmental factors affecting the soil bacterial community (35). The optimum temperature for nitrogen fixers' growth and activity is between 10 and 25°C (this is the temperature in the field between June and September) (3,17). The temperature in the field on the March sampling date was approximately 4.5°C.…”

Section: Discussionmentioning

confidence: 99%

“…The average numbers of copies of the nifH gene on the March sampling date were 7.45 ϫ 10 5 g Ϫ1 soil, and even on the June sampling date, copy number had not yet recovered (5.7 ϫ 10 5 copies g Ϫ1 soil); however, by September the population had increased 14-fold to 1.1 ϫ 10 7 copies g Ϫ1 soil. Eckford et al (17) suggested that the free-living diazotrophs may only be active seasonally in situ. Interestingly, the populations of the total metabolically active bacteria were not affected by seasonal variations in temperature, with populations only ranging between 4.5 ϫ 10 7 and 5.2 ϫ 10 7 copies g Ϫ1 soil on the three sampling dates.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Activity of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in Organic and Conventionally Managed Soils

Orr

James

Leifert

et al. 2011

Appl Environ Microbiol

148

View full text Add to dashboard Cite

Agricultural soils are heterogeneous environments in which conditions affecting microbial growth and diversity fluctuate widely in space and time. In this study, the molecular ecology of the total bacterial and free-living nitrogen-fixing communities in soils from the Nafferton Factorial Systems Comparison (NFSC) study in northeast England were examined. The field experiment was factorial in design, with organic versus conventional crop rotation, crop protection, and fertility management factors. Soils were sampled on three dates (March, June, and September) in 2007. Total RNA was extracted from all soil samples and reverse transcribed. Denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) were used to analyze nifH and 16S rRNA genes in order to study free-living diazotrophs and the total bacterial community, respectively. Crop rotation was shown to have a significant effect on total bacterial diversity (and that of free-living N fixers) (P < 0.001). On all three dates, nifH activity was higher in the conventional crop rotation. In contrast, qPCR analysis of free-living N fixers indicated significantly higher levels of activity in conventionally fertilized plots in June (P ‫؍‬ 0.0324) and in plots with organic crop protection in September (P ‫؍‬ 0.0143). To our knowledge, the effects of organic and conventional farming systems on free-living diazotrophs have never been studied. An increased understanding of the impacts of management practices on free-living N fixers could allow modifications in soil management practices to optimize the activity of these organisms.

show abstract

“…The results of human activity potentially include physical disruption of surface soils, eutrofication of soils and water systems, and contamination by detritus, xenobi-otic chemicals and non-indigenous microorganisms. Microbial contamination has been detected at both the macroscopic (Eckford et al 2002) and microscopic levels. For example, there are numerous reports on the isolation or detection of non-indigenous enteric bacteria derived from human faecal waste (McFeters et al 1993;Edwards et al 1998;Sjoling and Cowan 2000;Bruni et al 1997;Baker et al 2003).…”

Section: Results)mentioning

confidence: 99%

Dissemination and survival of non-indigenous bacterial genomes in pristine Antarctic environments

Tow

Cowan

2005

Extremophiles

View full text Add to dashboard Cite

show abstract

Free-Living Heterotrophic Nitrogen-Fixing Bacteria Isolated from Fuel-Contaminated Antarctic Soils

Cited by 90 publications

References 24 publications

Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

Indigenous hydrocarbon-utilizing bacterioflora in oil-polluted habitats in Kuwait, two decades after the greatest man-made oil spill

Diversity and Activity of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in Organic and Conventionally Managed Soils

Dissemination and survival of non-indigenous bacterial genomes in pristine Antarctic environments

Contact Info

Product

Resources

About