In this paper, we study the partial bi-free S-transform of a pair (a, b) of random variables, and the S-transform of the 2 × 2 matrix-valued random variable a 0 0 b associated with (a, b) 1 )ϕ(b n 1 ), for all m, n = 1, 2, · · · ). We thus find a lot of bi-free pairs of random variables to which the S-transforms of the corresponding matrix-value random variables do not satisfy Dykema's twisted multiplicative formula. Finally, if both (a 1 , b 1 ) and (a 2 , b 2 ) have factoring two-band moments, we prove that the Ψ-transforms of X 1 , X 2 , and X 1 X 2 satisfy a subordination equation.