Free-radical degradation of high-molar-mass hyaluronan induced by ascorbate plus cupric ions: Testing of stobadine and its two derivatives in function as antioxidants

Abstract

Stobadine·2HCl and its two hydrophilic derivatives SM1dM9dM10·2HCl and SME1i-ProC2·HCl were tested in the function of antioxidants on hyaluronan (HA) degradation induced by the Weissberger oxidative system [ascorbate plus Cu(II)]. As a primary method, rotational viscometry was applied, where the substance tested was added before or 1 h after the initiation of HA degradation. The most effective scavengers of •OH and peroxy-type radicals were recorded to be stobadine·2HCl and SME1i-ProC2·HCl, respectively. The most effective scavenger, determined by applying the ABTS assay, was stobadine·2HCl.

Oxidative Degradation of High-Molar-Mass Hyaluronan: Effects of Some Indole Derivatives to Hyaluronan Decay

“…The measurements of protective/promoting actions of indole derivatives ( 3 ), ( 4 ), and ( 5 ) against high-molar-mass hyaluronan oxidative degradation were performed analogously to the above study design [ 34 , 35 ]. The stock solutions of compounds ( 3 ), ( 4 ), and ( 5 ) were 160 mmol/L.…”

Hyaluronan and synovial joint: function, distribution and healing

“…Alkoxy-type C fragments may continue the propagation phase of the free-radical hyaluronan degradation reaction. Both fragments are represented by reduced molar masses (Kogan, 2011; Rychly et al, 2006 ; Hrabarova et al, 2012 ; Surovcikova et al, 2012 ; Valachova et al, 2013b ; Banasova et al, 2012 ).…”