Free-radical production and oxidative reactions of hemoglobin.

Winterbourn, Christine C.

doi:10.1289/ehp.8564321

Cited by 153 publications

(75 citation statements)

References 75 publications

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“…Phenylhydrazine radical is the most damaging agent that can denature hemoglobin molecules. The oxidation of reactive sulfhydryl (S-H) groups creates disulfide bonds that may change the conformation of globin chains, resulting in precipitation of the hemoglobin molecules called Heinz bodies, then follows by membrane lipids oxidation and causing hemolysis (Winterbourn, 1985). Bioavailability differs greatly from one polyphenol to the other; therefore the most abundant polyphenols in plants are not leading to the highest concentrations of active metabolites in target cells.…”

Section: Resultsmentioning

confidence: 99%

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Sangkitikomol¹

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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Section: Resultsmentioning

confidence: 99%

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Sangkitikomol¹

2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

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“…Moreover, KBrO 3 is known to cause haemolysis. 24) Actually, lysis of red blood cells, of which membranes contain a-tocopherol as a chainbreaking antioxidant, is induced simultaneously with oxidative stress by vitamin E deficiency. 25) As shown in Table 1, the KBrO 3 -induced elevation of MetHb concentration was prevented by the pre-administration of the GPx mimic ebselen.…”

Section: Resultsmentioning

confidence: 99%

Contribution of Nitric Oxide to Potassium Bromate-Induced Elevation of Methaemoglobin Concentration in Mouse Blood.

Watanabe

Togashi

Fukui

2002

Biological & Pharmaceutical Bulletin

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Bromate is frequently detected in tap water and bottled water, since it is an inorganic oxyhalide disinfection by-product, which is formed when drinking water supplies undergo ozonation, an advanced wastewater treatment technique. 1,2) Potassium bromate (KBrO 3 ) is a renal carcinogen and is classified as a group 2B (possibly carcinogenic to humans) by the International Agency for Research on Cancer, 3,4) as are bleomycin and carbon tetrachloride, etc. However, KBrO 3 is generally used as an oxidizing agent for the treatment of wheat flour and as a constituent in cold-wave hair solutions. Acute exposure to KBrO 3 causes not only kidney failure 5,6) but also neuropathological disorder, such as vertigo, tinnitus, and hearing loss. 7,8) Potassium bromate is known to cause oxidative modification of lipid, protein and DNA bases in the kidney, which has usually been thought to contribute to its renal carcinogenic properties.9,10) Methaemoglobinemia and cyanosis in the incipient stage of renal damage are also known to be caused by KBrO 3 .11) Acquired but not heritable methaemoglobinemia is thought to be caused by the oxidative modification of haemoglobin (Hb) by certain chemicals, such as amyl nitrite and acetanilide, since the iron in the haem rings of Hb is in the Fe 2ϩ state and Hb oxidation is speeded up by the presence of nitrite or certain transition metal ions, especially copper.12) So far, KBrO 3 has been regarded as the direct oxidant of Hb as chlorate 13) but no details of KBrO 3 -induced elevation of methaemoglobin (MetHb) concentration in blood have been examined. Previously KBrO 3 -induced renal oxidative stress and kidney damage were reported to be mainly attributable to the reduction of cytoplasmic glutathione peroxidase (GPx) activity, probably caused by the enhanced production of NO and peroxynitrite (ONOO Ϫ ).14) Therefore, it is possible that KBrO 3 -induced methaemoglobinemia is also related to the change in GPx activity resulting from the enhancement of NO production in blood vessels. In this report, the effects of the GPx mimic ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one) on the elevation of MetHb concentration, the enhancement of NO production and the attenuation of GPx activity by KBrO 3 are examined to clarify the significant impact of NO on KBrO 3 -induced methaemoglobinemia. In addition, the effect of ebselen on KBrO 3 -induced renal oxidative stress and kidney damage was comparatively investigated. MATERIALS AND METHODS ChemicalsGlutathione reductase from bakers yeast (EC 1.6.4.2), nitrate reductase from aspergillus species (EC 1.6.6.2), Hb, MetHb, reduced glutathione (GSH) and oxidized glutathione were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). 2,4-Dinitrophenylhydrazine (DNPH) was obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). KBrO 3 , potassium cyanide, sodium hydrosulfite and ebselen were purchased from Wako Pure Chemical Industries (Osaka, Japan). 1-Hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene (NOC-7), 3-(4-morpholinyl) sydnonimine hydroc...

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“…Through the combined activities ofthe hexose monophosphate shunt and glutathione peroxidase, superoxide dismutase, and catalase, they are able to protect themselves against oxygen reduced products (O-, H202, and derived species). Hemoglobin may also remove°2 or H202, since it can function catalytically as an oxidase or a peroxidase (2,3).…”

Section: Introductionmentioning

confidence: 99%

Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical.

Winterbourn¹,

Stern²

1987

J. Clin. Invest.

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161

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The ability of intact human red cells to scavenge extracellularly generated H202 and Oj, and to prevent formation of hydroxyl radicals and hypochlorous acid has been examined. Red cells inhibited oxidation of ferrocytochrome c by H202. Cells treated with aminotriazole no longer inhibited, indicating that protection was almost entirely due to intracellular catalase. Contribution by the GSH system was slight, and apparent only with low H202 concentrations when catalase was inhibited by aminotriazole. The cells were about a quarter as efficient at inhibiting cytochrome c oxidation as an equivalent concentration of purified catalase. No inhibition of 0--dependent reduction of ferricytochrome c or nitroblue tetrazolium was observed, although extracted red cell superoxide dismutase inhibited nitroblue tetrazolium reduction at one fortieth the concentration of that in the cells. Red cells efficiently inhibited deoxyribose oxidation by hydroxyl radicals generated from

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Free-radical production and oxidative reactions of hemoglobin.

Cited by 153 publications

References 75 publications

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Antioxidants in Thai Herb, Vegetable and Fruit Inhibit Hemolysis and Heinz Body Formation in Human Erythrocytes

Contribution of Nitric Oxide to Potassium Bromate-Induced Elevation of Methaemoglobin Concentration in Mouse Blood.

Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical.

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