Free radical scavengers in susceptible/resistant Biomphalaria alexandrina snails before and after infection

Mahmoud, Ahlam H.; Rizk, Maha Z.

doi:10.1016/j.cca.2004.08.012

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…In B . glabrata snails, it has been previously shown that the differential capability of host defense responses in susceptible and resistant snails could have different effects on enzyme activity due to the differences in the levels of these enzymes in each strain [31, 79, 80]. Serpin is a serine protease inhibitor that was only found expressed in plasma with an up-regulated expression profile at 24 and 48 hpi.…”

Section: Discussionmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Cambodia). Chronic O. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma (a fatal type of bile duct cancer). Infectivity of the parasite in the snail host is strongly correlated with destruction of helminths by the snail’s innate immune system, composed of cellular (hemocyte) and humoral (plasma) defense factors. To better understand this important host-parasite interface we applied sequential window acquisition of all theoretical spectra mass spectrometry (SWATH-MS) to identify and quantify the proteins from the hemolymph of B. siamensis goniomphalos experimentally infected with O. viverrini and compare them to non-infected snails (control group). A total of 362 and 242 proteins were identified in the hemocytes and plasma, respectively. Of these, 145 and 117 proteins exhibited significant differences in expression upon fluke infection in hemocytes and plasma, respectively. Among the proteins with significantly different expression patterns, we found proteins related to immune response (up-regulated in both hemocyte and plasma of infected snails) and proteins belonging to the structural and motor group (mostly down-regulated in hemocytes but up-regulated in plasma of infected snails). The proteins identified and quantified in this work will provide important information for the understanding of the factors involved in snail defense against O. viverrini and might facilitate the development of new strategies to control O. viverrini infection in endemic areas.

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Section: Discussionmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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“…The host, in turn, uses a complex network of interactions and free radical weapons against the parasite. In the case of schistosomiasis, these defence mechanisms succeed in encapsulating or eliminating the parasites in resistant snails but fail to deal with them in susceptible snails due to differences in the levels of endogenous oxidant enzymes (Mahmoud & Rizk 2004). This difference between susceptible and resistant snails may lead to different responses by the main host after vaccination and different degrees of protection from the parasites.…”

Section: Discussionmentioning

confidence: 99%

Biomphalaria alexandrina snails as immunogens against Schistosoma mansoni infection in mice

Hamed

Ali

Aly

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…As scavengers of H 2 O 2 , these enzymes are pivotal in the varied pathways involved in reactive oxygen species (ROS) detoxification. In the hemolymph of another snail host of this parasite, B. alexandrina , the free radical scavengers, catalase and glutathione peroxidase (GPx), were found to be higher in the hemolymph of individual snails that were more resistant to infection, while superoxide dismutase was lower in those snails susceptible to infection [58]. These enzymes varied in expression in both resistant and susceptible B. alexandrina snails after infection.…”

Section: Discussionmentioning

confidence: 99%

Biomphalaria glabrata peroxiredoxin: Effect of Schistosoma mansoni infection on differential gene regulation

Knight

Raghavan

Goodall

et al. 2009

Molecular and Biochemical Parasitology

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To identify gene(s) that may be associated with resistance/susceptibility in the intermediate snail host Biomphalaria glabrata to Schistosoma mansoni infection, a snail albumen gland cDNA library was differentially screened and a partial cDNA encoding an antioxidant enzyme thioredoxin peroxidase (Tpx), or peroxiredoxin (Prx), was identified. The 753 bp full-length, single-copy, constitutively expressed gene now referred to as BgPrx4 was later isolated. BgPrx4 is a 2-Cys peroxiredoxin containing the conserved peroxidatic cysteine (C P ) in the N-terminus and the resolving cysteine (C R ) in the C-terminus. Sequence analysis of BgPrx4 from both resistant and susceptible snails revealed the presence of several (at least 7) Single Nucleotide Polymorphisms (SNPs). Phylogenetic analysis indicated BgPrx4 to resemble a homolog of human peroxiredoxin, PRDX4. Northern analysis of hepatopancreas RNA from both resistant and susceptible snails showed that upon parasite exposure there were qualitative changes in gene expression. Quantitative real-time RT-PCR analysis showed differences in the levels of BgPrx4 transcript induction following infection, with the transcript up-regulated in resistant snails during the early phase (5 h) of infection compared to susceptible snails in which it was down-regulated within the early time period. While there was an increase in transcription in susceptible snails later (48 h) post-infection, this never reached the levels detected in resistant snails. A similar trend -higher, earlier up-regulation in the resistant snails but lower, slower protein expression in susceptible snails -was observed by Western blot analysis. Enzymatic analysis of the purified, recombinant BgPrx4 revealed the snail sequence to function as Prx but with an unusual ability to use both thioredoxin and glutathione as substrates.

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Free radical scavengers in susceptible/resistant Biomphalaria alexandrina snails before and after infection

Cited by 9 publications

References 25 publications

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Biomphalaria alexandrina snails as immunogens against Schistosoma mansoni infection in mice

Biomphalaria glabrata peroxiredoxin: Effect of Schistosoma mansoni infection on differential gene regulation

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