Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Lee, Si Eun; Ju, Eun Mi; Kim, Jeong Hee

doi:10.1038/emm.2001.43

Cited by 69 publications

(43 citation statements)

References 33 publications

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“…The EtOAc soluble fraction (6.9 g) was chromatographed on a Sephadex LH-20 column [4.0 × 68.0 cm (Pharmacia Biotech AB, Uppsala, Sweden); stepwise gradient of 50% to 100% methanol] to yield nineteen fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. HPLC [µ-Bondapak C 18 , 7.8 × 300 mm (Waters, Milford, MA, USA); 1% acetic acid in 35% methanol; 1.5 ml / min] of Fr.…”

Section: Chemicalsmentioning

confidence: 99%

Neuroprotective Effect of Smilacis chinae Rhizome on NMDA-Induced Neurotoxicity In Vitro and Focal Cerebral Ischemia In Vivo

et al. 2008

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Abstract. Previous work has shown that the Smilacis chinae rhizome (SCR) markedly inhibits amyloid β protein (25-35)-induced neuronal cell damage in cultured rat cortical neurons. The present study was conducted to further verify the neuroprotective effect of SCR on excitotoxic and cerebral ischemic injury using both in vitro and in vivo studies. Exposure of cultured cortical neurons to 1 mM N-methyl-D-aspartate (NMDA) for 12 h induced neuronal cell death. SCR (10 and 50 µg / ml) inhibited NMDA-induced neuronal death, elevation of intracellular calcium ([Ca 2+ ] i ), and generation of reactive oxygen species (ROS) in primary cultures of rat cortical neurons. In vivo, SCR prevented cerebral ischemic injury induced by 3-h middle cerebral artery occlusion (MCAO) and 24-h reperfusion. The ischemic infarct was significantly reduced in rats that received SCR (30 and 50 mg / kg, orally), with a corresponding improvement in neurological function. Moreover, SCR treatment significantly decreased the histological changes observed following ischemia. Oxyresveratrol and resveratrol isolated from SCR also inhibited NMDAinduced neuronal death, increase in [Ca 2+ ] i , and ROS generation in cultured cortical neurons, suggesting that the neuroprotective effect of SCR may be attributable to these compounds. Taken together, these results suggest that the neuroprotective effect of SCR against focal cerebral ischemic injury is due to its anti-excitotoxic effects and that SCR may have a therapeutic role in neurodegenerative diseases such as stroke.

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Section: Chemicalsmentioning

confidence: 99%

Neuroprotective Effect of Smilacis chinae Rhizome on NMDA-Induced Neurotoxicity In Vitro and Focal Cerebral Ischemia In Vivo

et al. 2008

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“…It has been shown that free radical scavengers and antioxidants are useful in protecting against cadmium toxicity [8]. Great efforts have been made in an attempt to find safe and potent natural antioxidants from plant sources [9]. Ocimum sanctum Linn.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activities of Hydroalcoholic Extract of Ocimum sanctum Against Cadmium Induced Toxicity in Rats

Ramesh

Satakopan

2010

Indian J Clin Biochem

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The present study was undertaken to analyze the antioxidant (both enzymic and nonenzymic) activities of leaves of Ocimum sanctum hydroalcoholic extract against cadmium induced damage in albino rats. Oral administration of cadmium as CdCl 2 (6.0 mg/kg body weight) led to significant elevation of lipid peroxidation (LPO) levels and significantly decreased Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPx), Reduced Glutathione (GSH) and Vitamin C (Ascorbate) levels. Administration of Ocimum sanctum extract (100 mg/kg body weight, po) and (200 mg/kg body weight, po) before and after cadmium intoxication showed a significant decrease in LPO levels and significant increase in SOD, CAT, GPx, GSH and Ascorbate levels. The results suggest that oral administration of Ocimum sanctum extract provides significant protection against cadmium induced toxicity in Wistar albino rats.

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“…The catalase (CAT) activity also showed significant (p<0.05) increase in hepatotoxic rats treated with varying dose of the extract compared with the hepatotoxic rats. Cellular radical scavenging systems include the enzymes such as SOD which scavenges the superoxide anions by catalyzing its dismutation and catalase (CAT) a haem enzyme which removes hydrogen peroxide [21]. Therefore, reduction in the activity of these enzymes (SOD and CAT) results in a number of deleterious effects due to the accumulation of superoxide anion and hydrogen peroxide.…”

Section: Effect Of Methanolic Extract Of Citulus Lanatus Seeds On Oximentioning

confidence: 99%

Effect of methanolic extract of Citrulus lanatus Seed on lipid profile and oxidative stress in Acetaminophen intoxicated Rats

Omoboyowa¹

2016

abp

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The effect of methanolic extract of Citrullus lanatus seed on lipid profile and anti-oxidants in acetaminophen-induced hepatotoxic rats was investigated in the present study. A total of twenty (20) male Wistar rats were divided into five groups (n = 4). Group I served as normal control and were not exposed to paracetamol. Group II was exposed to 750 mg/kg body weight of paracetamol( intoxicated group),. Group III was administered with 0.5 ml distilled water, 5 mg/kg b. w of silymarin and group IV and V were administered with200 mg/kg and 400 mg/kg body weight of C. lanatus methanolic seed extract respectively.. We found that C. lanatus extract is safe at a dosage of up to 5000 mg/kg body weight. The biochemical analysis of current study showed significant (p<0.05) decrease in total cholesterol of the rats exposed to Paracetamol and treated with the plant extract compared with the rats exposed to Paracetamol only. The rats exposed to 750 mg/kg b.w. of Paracetamol and treated with varying doses of the extract showed non-significant (p>0.05) increase in VLDL, HDL and TAG concentration compared with the rats exposed to Paracetamol and 0.5 ml of distilled water (Vehicle). Moreover the animals exposed to 750 mg/kg b.w. of paracetamol and treated with 200 and 400 mg/kg b.w. of the extract showed significant (p< 0.05) increase in the antioxidant parameters compared to the animals exposed to 750 mg/kg b.w of paracetamol and0.5 ml of distilled water. The increase in the lipid profile of the treated animals reveal the ameliorating effect of dyslipidemia as observed in paracetamol intoxicated animals. Taken together methanolic seed extract of C. lanatus can substantiate its use as therapeutic agent against lipid imbalance and free radical generation in traditional system of medicine.

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Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Cited by 69 publications

References 33 publications

Neuroprotective Effect of Smilacis chinae Rhizome on NMDA-Induced Neurotoxicity In Vitro and Focal Cerebral Ischemia In Vivo

Neuroprotective Effect of Smilacis chinae Rhizome on NMDA-Induced Neurotoxicity In Vitro and Focal Cerebral Ischemia In Vivo

Antioxidant Activities of Hydroalcoholic Extract of Ocimum sanctum Against Cadmium Induced Toxicity in Rats

Effect of methanolic extract of Citrulus lanatus Seed on lipid profile and oxidative stress in Acetaminophen intoxicated Rats

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