Free Radical Scavenging, Metal Chelating and Singlet Oxygen Quenching Activity of Fractionated Brown Seaweed Sargassum hystrix Extract

Budhiyanti, Siti Ari; Raharjo, Sri; Marseno, Djagal Wiseso; Lelana, Iwan Yusuf Bambang

doi:10.3923/jbs.2011.288.298

Cited by 30 publications

(26 citation statements)

References 63 publications

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“…The coastlines with rocky shores at Java Island Indonesia, Gunung Kidul (Yogyakarta) and Jepara (Central Java), have abundant resources of seaweed, especially brown algae Sargassum sp., however little effort has been made to explore the antioxidant potential of seaweed harvested from these area. Sargassum had been studied extensively showing high antioxidant potential in vitro (Lim et al, 2002;Santoso et al, 2004;Kim et al, 2005;Park et al, 2005;Cho et al, 2007;Zubia et al, 2007;2008;Budhiyanti et al, 2011). Antioxidant compounds play an important role against various diseases (e.g., atherosclerosis, chronic inflammation, cardiovascular disorders and cancer) and aging processes (Kohen and Nyska, 2002).…”

Section: Introductionmentioning

confidence: 99%

ANTIOXIDANT ACTIVITY OF BROWN ALGAE SARGASSUM SPECIES EXTRACTS FROM THE COASTLINE OF JAVA ISLAND

Budhiyanti¹,

Raharjo²,

Marseno³

et al. 2012

American Journal of Agricultural and Biological Sciences

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The coastlines with rocky shores at Java Island Indonesia, Gunung Kidul (Yogyakarta) and Jepara (Central Java), have abundant resources of brown algae Sargassum sp. Little effort, however, has been made to explore the antioxidant potential of the algae harvested from these area. Tropical brown algae have proven to produce a very effective antioxidant defence system due to the strong UV radiation in the tropical environment. The Total Phenolic Content (TPC) of the extracts were evaluated by using Follin Ciocalteau reagent, while antioxidant activities were evaluated by using 2,2-Diphenyl-1-Picrylhydrzyl Radical Scavenging Activity (DPPH-RSA) and Ferrous Ion-Chelating (FIC) ability. The effects of treatments on TPC and antioxidant activities were analysed using analysis of variance models. There were four treatments and three replicates. These treatments were extract types (cytoplasmic and membrane bound), harvest sites (Gunung Kidul and Jepara), harvest seasons (dry and rainy) and various Sargassum species. The TPC of extracts varied from 0.006-0.65 and 6.72-21.99 g phloroglucinol equivalent/100 g dried extract for cytoplasmic and membrane bound extract, respectively. The DPPH-RSA in extracts concentration of 0.45 mg mL −1 were in the range of 14.61-48.71 % for membrane bound and 0.17-44.05 % for cytoplasmic extracts. The FIC in extract concentration of 3.3 mg mL -1 were in the range of 2.0512.51 and 26.77-68.80 % for cytoplasmic and membrane bound extract, respectively. Nevertheless, these extracts had lower activity when compared with BHT and EDTA as positive control of RSA-DPPH and FIC, respectively. The antioxidant activities of Sargassum were influenced by types of extracts, harvest sites, seasons and species. The antioxidant activity of membrane bound was higher than cytoplasmic extract. The increasing of DPPH-RSA and FIC were proportional to TPC. The highest antioxidant activity was found at S. hystrix from Gunung Kidul area harvested during dry season.

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Section: Introductionmentioning

confidence: 99%

ANTIOXIDANT ACTIVITY OF BROWN ALGAE SARGASSUM SPECIES EXTRACTS FROM THE COASTLINE OF JAVA ISLAND

Budhiyanti¹,

Raharjo²,

Marseno³

et al. 2012

American Journal of Agricultural and Biological Sciences

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“…was about 800 ppm (Ale et al, 2011), sulphated polysaccharides of Fucus vesiculosus was about 800 ppm (Suresh et al, 2013), sulphated polysaccharides of S. pallidum was about 1,000 ppm , and sulphated polysaccharides of S. plagiophyllum was about 700 ppm (Suresh et al, 2013). IC 50 value of S. hystrix dry powder was lower than sulphated polysaccharides of Ecklonia cava was about 43.9-100 ppm (Athukorala et al, 2009), sulphated polysaccharides of Cladosiphon okamuranus was about 100 ppm (Teruya et al, 2007), and S. hystrix extract was about 0.33±0.03 mg/ml (Budhiyanti et al, 2011).…”

Section: Antioxidant Activitymentioning

confidence: 88%

Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

Lailatussifa

Husni

Isnansetyo

2017

JFS

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AbstrakPenelitian ini bertujuan untuk mengetahui aktivitas antioksidan, kandungan total fenol, kandungan nutrisi, dan kandungan fitokimia dari bubuk kering Sargassum hystrix. Ekstrak air dari sampel kering alga cokelat (S. hystrix) disiapkan dan diuji kandungan total fenolnya. Jumlah kandungan total fenol ekstrak air yaitu 11,43 g Gallic Acid Equivalent (GAE)/100 g berat kering sampel dengan menggunakan metode Folin-Ciocalteu. Aktivitas antioksidan (Radical Scavenging Activity/RSA) BHT yang digunakan sebagai standar tergolong tinggi, penghambatan radikal DPPH pada 1 mg sampel kering/ml pelarut yakni 96%. Nilai IC 50 BHT adalah sebesar 0,227 ± 0,001 mg sampel kering/ml pelarut, sedangkan aktivitas penangkapan radikal (RSA) bubuk kering S. hystrix sebesar 65,28% pada 1 mg sampel kering/ml pelarut. Nilai IC 50 bubuk kering S. hystrix adalah sebesar 0,616 ± 0,005 mg sampel kering/ml pelarut. Kandungan nutrisi dan komponen bioaktif bubuk kering S. hystrix seimbang untuk mendukung aktivitas antioksidan sampel. Hasil ini menunjukkan bahwa alga tersebut potensial sebagai sumber antioksidan alami. Kata kunci: Aktivitas antioksidan, bubuk kering Sargassum hystrix, fitokimia, kandungan fenolik, kandungan nutrisi AbstractThis research aims to know antioxidant activity, total phenolic, nutrient, and screening phytochemistry compounds of S. hystrix dry powder. Water extract from the dried sample of brown algae (S. hystrix) was prepared and examined for its phenolic compounds. The amount of total phenolic compounds in water extract was about 11.43 g Gallic Acid Equivalent (GAE)/100 g of dry basis sample, as measured by using Folin-Ciocalteu method. The antioxidant activity (Radical Scavenging Activity/RSA) of BHT as a standard was high about 96% inhibition of radical DPPH with 1 mg dry sample/ml solvent. The IC 50 of the BHT was 0.227 ± 0.001 mg of dry sample/ ml solvent. While the radical activity (RSA) of S. hystrix dry powder was about 65.28% at 1 mg dry sample/ml solvent. The IC 50 of S. hystrix dry powder was 0.616 ± 0.005 mg of dry sample/ml solvent. Nutrient contents and bioactive compounds of dry powder S. hystrix were balanced to support antioxidant activity of the sample. The results suggest that this alga is a potential source of natural antioxidant.

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“…The Sargassum genus, such as brown algae, has shown a high antioxidant potential in vitro [9]. Phenolic compound from the brown algae Sargassum hystrix from Yogyakarta, Indonesia, showed a high antioxidant activity in vitro [9]. The main phenolic compounds that were detected in brown algae were phlorotannins.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is not enough literature or data on the presence of polyphenolic compounds as well as the antioxidant activity of various seaweeds, especially tropical [8]. The Sargassum genus, such as brown algae, has shown a high antioxidant potential in vitro [9]. Phenolic compound from the brown algae Sargassum hystrix from Yogyakarta, Indonesia, showed a high antioxidant activity in vitro [9].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Lipid Peroxidation Induced by Ultraviolet Radiation by Crude Phlorotannis Isolated from Brown Algae Sargassum hystrix v. buxifolium C. Agardh

Harnita¹,

Santosa²,

Martono³

et al. 2013

Indones. J. Chem.

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This study examines the antioxidant activity of crude phlorotannins from the brown algae Sargassum hystrix v. buxifolium (Chauvin) J. Agardh, through the inhibition of a lipid peroxidation reaction that is induced by the UV radiation. The antioxidant activity during the UV exposure was investigated using the laser-based photoacoustic method for the detection of the ethylene as indicator for lipid peroxidation. This involves an experiment that isolated crude phlorotannins from the ethyl acetate fraction of the Sargassum hystrix methanol extract, hereafter referred to as PFSH. It results in the antioxidant activity as a potent lipid peroxidation inhibitor. Statistically, such antioxidant activity is not significantly different than the commercial antioxidant, which is vitamin C (p > 0.05). The amount of the total phlorotannins, using the Folin-Ciocalteu method, was measured to be approximately 0.13% w/w. In addition, it is found that PFSH contains phlorotannins with low molecular weight (MW) (<1000 Da). Keywords

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Free Radical Scavenging, Metal Chelating and Singlet Oxygen Quenching Activity of Fractionated Brown Seaweed Sargassum hystrix Extract

Cited by 30 publications

References 63 publications

ANTIOXIDANT ACTIVITY OF BROWN ALGAE <i>SARGASSUM SPECIES</i> EXTRACTS FROM THE COASTLINE OF JAVA ISLAND

ANTIOXIDANT ACTIVITY OF BROWN ALGAE <i>SARGASSUM SPECIES</i> EXTRACTS FROM THE COASTLINE OF JAVA ISLAND

Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

Inhibition of Lipid Peroxidation Induced by Ultraviolet Radiation by Crude Phlorotannis Isolated from Brown Algae <i>Sargassum hystrix v. buxifolium</i> C. Agardh

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