Free Radicals Scavenging and Protein Protective Property of Ocimum sanctum (L)

Abstract

Ocimum sanctum (L) is a well-known herbal plant used for the treatment of bronchitis, malaria, diarrhea, dysentery, skin disease, arthritis, eye diseases and insect bites. It is proposed to have anticancer, antidiabetic, antifungal, antimicrobial, cardio protective, analgesic and antispasmodic action. In our present study, we have tried to explore its antioxidant, free radical scavenging and its ability to prevent damage to protein from free radicals. The antioxidant and free radical scavenging activity of Ocimum sanctum leaves was determined using ferric thiocyanate, thiobarbituric Acid, 2, 2-diphenyl-1-picrylhydrazyl, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods. Further, its protein protective property was evaluated using UV-VIS spectrophotometer methods. The results of ferric thiocyanate and 2, 2-diphenyl-1-picrylhydrazyl methods were determined that O. sanctum have strong antioxidant activity (92.12%) and free radicals scavenging property (50.74%) respectively. Further, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods determined that extracts (100 µg/ml) have hydrogen peroxide (20.12%), hydroxyl radicals (12.68%) and superoxide radicals (21.68%) scavenging

Effects of sodium fluoride and Ocimum sanctum extract on the lifespan and climbing ability of Drosophila melanogaster

“…Free radical scavenging activity is a chief mechanism through which Ocimum sanctum products protect against cellular damage. Its role in free radicals scavenging property has confirmed its strong antioxidant activity and free radicals scavenging property (Ganasoundari et al, 1998;Keshari, Srivastava, Verma, & Srivastava, 2016).…”

Phytochemical and GC-MS Analysis of Hydro Ethanolic Leaf Extract of Ocimum sanctum (L.)

“…Extract was prepared by the established protocol of Keshari et al 2017 with certain modification. [6] Fresh and healthy young leaves collected from the Botanical Garden were washed under running water and further with deionised water and then dried in shade and then at 37°C in incubator. Dried leaves were further crushed with the help of mixer grinder.…”

“…The preliminary screening was performed for the phytochemical analysis of the crude extracts to identify the various phytoconstituents using standard procedures as described by Keshari A et al and Babu N. R. et al [6,7] Detection of tannins, phlobatanins, saponinsm, alkaloids, flavonoids, quinine, anthraquinones coumarins, sterols, glycosides, terpenoids, diterpenes, triterpenes, phenols, starch, carbohydrates, proteins was performed.…”

“…The prepare extract was used for phytochemical and GC-MS analysis. Results: In qualitative phytochemical analysis tannins, carbohydrate, quinine, anthraquinones, coumarins, phlobatanins, alkaloids, flavonoids, glycosides, terpenoids, diterpenes, phenols were found present while GC-MS analysis showed Eugenol, Cyclohexane,4,11, Oxatricyclo[8.2.0.0(4,6)]dodecane,,12-trimethyl-9-methylene, Tetracontane, Phytol were present in majority. Conclusion: The study concluded that O. sanctum leaf extracts contain many biological active compounds which could be exploited for a development of plant based drug.…”

Ocimum sanctum: Role in Diseases Management Through Modulating Various Biological Activity

“…Additional, hydrogen peroxide, hydroxyl radical and superoxide radical scavenging methods determined that extracts with concentration100 μg/ml have hydrogen peroxide (20.12%), hydroxyl radicals (12.68%) and superoxide radicals (21.68%) scavenging activity respectively. 14 Several diseases like cancer, diabetes etc. have been reported to be linked with inflammatory processes.…”