Free‐Standing Gold Nanoparticle Membrane by the Spontaneous Reduction of Aqueous Chloroaurate Ions by Oxyethylene‐Linkage‐Bearing Diamine at a Liquid–Liquid Interface

Abstract

Electroporation: A BTX 600 electro cell manipulator was used to deliver PEG-QDs to HeLa cells. Electroporation parameters were varied using several solutions (phosphate-buffered saline, Krebs Ringers Buffer, Dulbecco's Modified Eagles' Medium, and HEPESbuffered (HEPESÐ4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid) isotonic glucose solution), charging voltages (100±500 V), and pulse lengths (0.5±20 ms). Optimal intracellular delivery without extensive cell death (greater than 50 % viability) occurred in PBS with a single 100±200 V, 1±5 ms pulse. Approximately 10 6 cells were suspended in 400 lL of DMEM with 250 lg mL ±1 QDs. The electroporation charge was applied, and, after 10 min, the cells were pelleted to remove the QD solution, and then plated on coverslips. All steps were performed at 4 C to prevent endocytosis of QDs.

Organic dye molecules as reducing agent for the synthesis of electroactive gold nanoplates

“…For example, the structural dependence of reducing agents on the shape selective growth of nanocrystals is either little known or ignored so far. Significantly, our earlier investigations and other related studies carried out with linear amines have demonstrated the formation of only spherical gold nanoparticles [30][31][32][33]. From these studies, we conclude that shape control can be achieved by choosing specific reducing agents during the growth of nanocrystals [34].…”

Hybrid Systems Biomolecule-Polymeric Nanoparticle: Synthesis, Properties and Biotechnological Applications

“…As part of our search for newer and more versatile materials with tailorable surfaces for cell immobilization, they describe herein the synthesis of a free-standing gold nanoparticles membrane whose surface may readily be modified to render it compatible for a variety of applications. More specifically, they demonstrate the formation of a self-supporting gold nanoparticle membrane at a liquid interface by the spontaneous reduction of aqueouschloroaurate ions by bis(2-(4-aminophenoxy)ethyl)ether (DAEE) present in chloroform [158]. The gold nanoparticle membrane is extremely stable, robust, easily handled, and malleable and can be grown over large areas and thickness by suitably varying the experimental conditions.…”

Conducting Polymers with Micro or Nanometer Structure

“…Recently, it has been demonstrated 4 AuCl could be reduced by amine-containing molecules including hexadecylaniline, diamine-containing oxyethylene linkage, resulting in metallic gold particles accompanied with the formation of corresponding polymers [174,175]. PANI nanostructures prepared by 4 AuCl as the oxidant were reported [176,177].…”

“…Nowadays various micro/nanostructures of conducting polymers, such as nanoparticles [173], nanofibers [174], nanotubes [19], nanowires [175], spheres [67,73] even dendrites [66,176] are easily prepared by "hard" or "soft" templates. However, 3D-microstructures assembled from 1D-nanotubes or nanofibers are actually needed because they provide high functionality and performance for their applications in technology.…”