Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury

Stokols, Shula; Tuszynski, Mark H.

doi:10.1016/j.biomaterials.2005.06.039

Cited by 282 publications

(208 citation statements)

References 32 publications

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“…Although its mechanical properties can be modified to mimic the mechanical properties of its host tissue (Balgude et al, 2001), an agarose ECM mimetic alone-when compared with the effectivity of other natural ECM mimeticsdoes not suffice to achieve significant degrees of neuronal growth (Lin et al, 2005). Via modification of the material prior to implantation, the neuronal growth rate through agarose scaffolds can, however, be increased (Gros et al, 2010;Jain et al, 2006;Lee et al, 2010;Sakiyama-Elbert et al, 2012;Stokols and Tuszynski, 2006).…”

Section: Agarosementioning

confidence: 99%

Neural ECM mimetics

Estrada

Tekinay

Müller

2014

Progress in Brain Research

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The consequence of numerous neurological disorders is the significant loss of neural cells, which further results in multilevel dysfunction or severe functional deficits. The extracellular matrix (ECM) is of tremendous importance for neural regeneration mediating ambivalent functions: ECM serves as a growth-promoting substrate for neurons but, on the other hand, is a major constituent of the inhibitory scar, which results from traumatic injuries of the central nervous system. Therefore, cell and tissue replacement strategies on the basis of ECM mimetics are very promising therapeutic interventions. Numerous synthetic and natural materials have proven effective both in vitro and in vivo. The closer a material's physicochemical and molecular properties are to the original extracellular matrix, the more promising its effectiveness may be. Relevant factors that need to be taken into account when designing such materials for neural repair relate to receptor-mediated cell-matrix interactions, which are dependent on chemical and mechanical sensing. This chapter outlines important characteristics of natural and synthetic ECM materials (scaffolds) and provides an overview of recent advances in design and application of ECM materials for neural regeneration, both in therapeutic applications and in basic biological research.

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Section: Agarosementioning

confidence: 99%

Neural ECM mimetics

Estrada

Tekinay

Müller

2014

Progress in Brain Research

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“…Freeze-drying is another way to fabricate uniaxial hydrogel scaffolds [37,38]. With a gradient in temperature, scaffolds with honeycomb structure were created [38]. Along with other factors, linear growth of axons within channels was observed and the axons successfully bridged the lesion.…”

Section: Architecturementioning

confidence: 99%

Hyaluronic acid-based scaffold for central neural tissue engineering

Wang

et al. 2012

Interface Focus.

129

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Central nervous system (CNS) regeneration with central neuronal connections and restoration of synaptic connections has been a long-standing worldwide problem and, to date, no effective clinical therapies are widely accepted for CNS injuries. The limited regenerative capacity of the CNS results from the growth-inhibitory environment that impedes the regrowth of axons. Central neural tissue engineering has attracted extensive attention from multi-disciplinary scientists in recent years, and many studies have been carried out to develop cell-and regenerationactivating biomaterial scaffolds that create an artificial micro-environment suitable for axonal regeneration. Among all the biomaterials, hyaluronic acid (HA) is a promising candidate for central neural tissue engineering because of its unique physico-chemical and biological properties. This review attempts to outline current biomaterials-based strategies for CNS regeneration from a tissue engineering point of view and discusses the main progresses in research of HA-based scaffolds for central neural tissue engineering in detail.

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“…These constructs had pore diameters (channels) of 119±26m and were capable of sustained growth factor release. Follow-up assessment of the scaffolds in a spinal cord injury paradigm showed organized axonal regeneration through the agarose channels (Stokols and Tuszynski 2006). In contrast to the brain, nerves within the PNS have a unique global structural arrangement.…”

Section: Nervous Systemmentioning

confidence: 99%