Freeze‐dried rehydrated human blood platelets regulate intracellular pH

Tang, Michelle; Wolkers, Willem F.; Crowe, John H.; Tablin, Fern

doi:10.1111/j.1537-2995.2006.00838.x

Cited by 21 publications

(18 citation statements)

References 49 publications

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“…A technique that combines multiple physical processes, lyophilization or freeze-drying, is widely used to preserve proteins at non-cryogenic temperatures and lyophilization methods have also been under development for various blood cell types [42–45]. During freeze-drying aqueous samples are first frozen to induce the formation of ice crystals in the preservation matrix.…”

Section: Cell Preservation Fundamentalsmentioning

confidence: 99%

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Wang

Elliott

2017

Curr Stem Cell Rep

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Purpose of the review Over the past several decades, cryopreservation has been widely used to preserve cells during long term storage, but advances in stem cell therapies, regenerative medicine, and miniaturized cell-based diagnostics and sensors are providing new targets of opportunity for advancing preservation methodologies. The advent of microfluidics-based devices is an interesting case in which the technology has been used to improve preservation processing, but as the devices have evolved to also include cells, tissues, and simulated organs as part of the architecture, the biochip itself is a desirable target for preservation. In this review, we will focus on the synergistic co-development of preservation methods and biochip technologies, while identifying where the challenges and opportunities lie in developing methods to place on-chip biologics on the shelf, ready for use. Recent findings Emerging studies are demonstrating that the cost of some biochips have been reduced to the extent that they will have high utility in point-of-care settings, especially in low resource environments where diagnostic capabilities are limited. Ice-free low temperature vitrification and anhydrous vitrification technologies will likely emerge as the preferred strategy for long-term preservation of bio-chips. Summary The development of preservation methodologies for partially or fully assembled biochips would enable the widespread distribution of these technologies and enhance their application.

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Section: Cell Preservation Fundamentalsmentioning

confidence: 99%

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Wang

Elliott

2017

Curr Stem Cell Rep

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“…Lyophilisation should be used when complete rehydration needed and product are heat liable, unstable, or high valuable. However, lyophilisation may also cause problems due to changes in liquid phase, solubility, and water freezing [44]. Thus, both processes have their own pros and cons and different application.…”

Section: Application Of Lyophilisation and Desiccation And Moleculmentioning

confidence: 99%

Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells

Bissoyi

Kumar

Rizvanov

et al. 2016

Stem Cells International

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Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.

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“…Another method is to freeze‐dry PLTs, which permits their storage for up to 1 year. Rehydrated lyophilized PLTs retain basic functions in vitro such as agglutination and thrombin generation or internal pH regulation . However, paraformaldehyde treatment combined or not with a cryoprotectant ‐ is required.…”

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confidence: 99%

Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo

et al. 2015

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Freeze‐dried rehydrated human blood platelets regulate intracellular pH

Abstract: The results show that the pHi regulation system is largely preserved during freeze-drying and rehydration of PLTs.

Cited by 21 publications

References 49 publications

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Synergistic Development of Biochips and Cell Preservation Methodologies: a Tale of Converging Technologies

Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells

Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo

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