Freeze drying (lyophilization) and freeze concentration

Berk, Zeki

doi:10.1016/b978-0-12-812018-7.00023-3

Cited by 28 publications

(34 citation statements)

References 23 publications

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“…It is very common to use milling and drying (hot air or freeze-drying) as conditioning steps for polyphenol extraction processes [2]. However, freeze-drying is an expensive process, which prevents it from being used at an industrial scale, specially for high water content products such as citrus fruits [28,29]. Hot-air drying is cheaper, but it has the disadvantage of exposing the sample to heat and oxygen for extended periods of time [2].…”

Section: Pilot-scale Extractionmentioning

confidence: 99%

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) that is Extendible to the Citrus Genus

et al. 2018

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The processing of Mexican limes generates great amounts of peel as a by-product. Lime peel is mainly rich in the flavonoid hesperidin, whose bioactivity is oriented mainly to cardiovascular diseases and cancer. The purpose of this work was to develop a green process for the extraction and purification of hesperidin from Mexican lime peel. The extraction of hesperidin was investigated on a laboratory scale by varying the solvent composition and the solid-to-solvent ratio, and then scaling this process (volume: 20 L). Next, a purification process using adsorption resins was assessed: first through static tests to determine the adsorption efficiency with two resins (FPX66, EXA118), and then on a packed column with 80 mL of resin at 25 °C. Lab-scale extraction showed that the best conditions were a solid-to-solvent ratio of 0.33 g/mL and 60% ethanol. After using these conditions at pilot scale and concentrating the solution, the hesperidin content of the extract was 0.303 mg/mL. Through static tests, higher adsorption efficiencies were achieved with the EXA-118 resin and diluted extract (4:6 ratio with 10% dimethylsulfoxide, (DMSO)). Finally, the purification process on a packed column from the diluted extract (hesperidin concentration of 0.109 mg/mL) had a mean recovery efficiency of almost 90%.

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Section: Pilot-scale Extractionmentioning

confidence: 99%

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) that is Extendible to the Citrus Genus

et al. 2018

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“…Freeze drying, or lyophilization, is a preservation process associated with food for minimum travel space, food for backpackers or 'space food'. Freeze-drying yields a lightweight, high-quality, and easily rehydrated product that retains the original shape and most of the original constituents of the starting material [5]. As it has been demonstrated, bioactive compounds and vitamins, which are part of the grape raw material, remain unstable during storage [6].…”

Section: Introductionmentioning

confidence: 99%

Entrapped Psychrotolerant Yeast Cells within Pine Sawdust for Low Temperature Wine Making: Impact on Wine Quality

2020

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An alternative methodology is proposed for low temperature winemaking using freeze-dried raw materials. Pine sawdust was delignified and the received porous cellulosic material was applied as immobilization carrier of the psychrotolerant yeast strain Saccharomyces cerevisiae AXAZ-1. The immobilization of yeast cells was examined and verified by scanning electron microscopy (SEM). The immobilized biocatalyst and high-gravity grape must were separately freeze-dried without cryoprotectants and stored at room temperature (20–22 °C) for 3 months. The effect of storage on the fermentation efficiency of the immobilized biocatalyst at low temperatures (1–10 °C), as well as on the aromatic characteristics of the produced wines was evaluated. Storage time had no significant effect on the fermentation efficiency of the biocatalyst resulting in most cases in high ethanol production 13.8–14.8% v/v. The volatile fraction of the produced wines was examined using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography mass spectrometry (GC/MS). GC-MS/SPME analysis along with the organoleptic evaluation revealed in all produced wines a plethora of fresh and fruit aromatic notes. To conclude, fermentation kinetics and aromatic profile evaluation encourages the production of high-quality sweet wines at low temperatures using pine sawdust (Pinus halepensis) entrapped yeast cells as a promoter.

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“…It is very common to use milling and drying (hot air or freeze drying) as conditioning steps for polyphenol extraction processes [2]. However, freeze drying is an expensive process, which prevents from using it at an industrial scale, specially for high water content products such as citrus fruits [28,29]. Hot air drying is cheaper, but it has the disadvantage of exposing the sample to heat and oxygen for extended periods of time [2].…”

Section: Pilot-scale Extractionmentioning

confidence: 99%

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (<em>Citrus aurantifolia</em> Swingle) Extendible to <em>Citrus </em>Genus

Rosa¹,

Ruiz-Palomino²,

Guatemala-Morales³

et al. 2018

Preprint

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The processing of Mexican limes generates great amounts of peel as a byproduct. Lime peel is mainly rich in the flavonoid hesperidin, whose bioactivity is oriented mainly to cardiovascular diseases and cancer. The purpose of this work was to develop a green process for the extraction and purification of hesperidin from Mexican lime peel. The extraction of hesperidin was investigated on a laboratory scale by varying the solvent composition and the solid-to-solvent ratio. The best conditions (solid-to-solvent ratio of 0.33 g/mL and 60% ethanol) were used for the extraction of hesperidin in a pilot scale (Volume = 20 L). The kinetics of the extraction was studied to find the maximum hesperidin concentration at 100 min. The concentrated extract had a hesperidin content of 0.303 mg/mL. Next, a purification process using adsorption resins was assessed. Through static tests, it was determined that higher adsorption efficiencies were achieved with the EXA-118 resin and diluted extract (4:6 ratio with 10% DMSO). Finally, the adsorption of hesperidin from the diluted extract (hesperidin concentration of 0.109 mg/mL) was carried out at 25 °C in a column packed with 80 mL of EXA-118 resin. The mean recovery efficiency of hesperidin from the extract was almost 90%.

show abstract

Freeze drying (lyophilization) and freeze concentration

Cited by 28 publications

References 23 publications

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) that is Extendible to the Citrus Genus

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (Citrus aurantifolia Swingle) that is Extendible to the Citrus Genus

Entrapped Psychrotolerant Yeast Cells within Pine Sawdust for Low Temperature Wine Making: Impact on Wine Quality

A Green Process for the Extraction and Purification of Hesperidin from Mexican Lime Peel (<em>Citrus aurantifolia</em> Swingle) Extendible to <em>Citrus </em>Genus

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