Freeze-Drying of Red Blood Cells at Ultra-Low Temperatures

Rindler, Volker; Lüneberger, S.; Schwindke, P.; Heschel, I.; Rau, G.

doi:10.1006/cryo.1998.2143

Cited by 44 publications

(21 citation statements)

References 19 publications

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“…The results demonstrated that a higher recovery of freezedried RBCs than other researches [16,17] was achieved. This study suggests that intracellular trehalose is beneficial for the freeze-drying of RBCs.…”

Section: Discussionmentioning

confidence: 61%

Intracellular trehalose improves the survival of human red blood cells by freeze-drying

Liu

Zeng

et al. 2007

Front. Energy Power Eng. China

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Freeze-drying of human red blood cells has a potential important application for blood transfusion. The aim of this study was to investigate the effects of intracellular trehalose on the survival of red blood cells after freeze-drying and rehydration. Fresh red blood cells were incubated in trehalose solutions of various concentrations at 37°C for 7 h following freeze-drying. Polyvinylpyrrolidone, Trehalose, sodium citrate, and human serum albumin were used as extracellular protective agents for the freeze-drying of red blood cells. The results indicated that the intracellular trehalose concentration was increased with increasing concentration of extracellular trehalose solution, and the maximum concentration of intracellular trehalose reached 35 mmol/L. The viability of freeze-dried red blood cells increased with the increment of intracellular trehalose concentration.

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Section: Discussionmentioning

confidence: 61%

Intracellular trehalose improves the survival of human red blood cells by freeze-drying

Liu

Zeng

et al. 2007

Front. Energy Power Eng. China

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“…We have observed that non-muscle contamination of the biopsies creates considerable variation in several assays, including insulin signaling measurements. Freeze-drying of muscle specimens allows dissection and purification of muscle from non-muscle contaminants [7][8][9]. This might reduce sample size and improve analytical precision, which would be especially desirable in human studies where such invasive procedures should be limited to minimum.…”

Section: Introductionmentioning

confidence: 99%

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Korsheninnikova

Seppälä-Lindroos

Vehkavaara

et al. 2002

Diabetes Metabolism Res

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“…[29][30][31][32] Other protective agents include hydroxyl-ethyl starch (HES), 33,34 bovine serum albumin (BSA), 35 human serum albumin (HSA), polyvinylpyrrolidone (PVP), and dimethyl sulfoxide (DMSO). 36 Even with the addition of protectants, little success has been observed after lyophilization and rehydration of various mammalian cells.…”

Section: Determination Of Lipid Peroxidation In Desiccated Red Blood mentioning

confidence: 99%

Determination of Lipid Peroxidation in Desiccated Red Blood Cells

Kanias

Wong

Acker

2007

Cell Preservation Technology

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Lipid peroxidation of biological membranes is one of the most studied indicators of oxidative stress. Very littleis known about the extent of oxidative damage that occurs during the desiccation of mammalian cells. The objective of this study was to modify and validate a method for the determination of lipid peroxidation in desiccated red blood cells (RBCs). The measurement of malondialdehydes (MDAs), the decomposition products of oxidized polyunsaturated fatty acids, is commonly used as a method for the quantification of lipid peroxidation. The method is based on the reaction of MDAs with thiobarbituric acid, which forms a thiobarbituric acid reactive substances (TBARS) chromophore. Validation of the method to dried RBC specimens included the optimization of an MDA standard curve, spiking studies, measurement of TBARS in lyophilized RBCs, and reexamination of the reagents and processes that are associated with this assay. The recovery of MDA standards, which were lyophilized with RBCs was reasonable (72.96% ؎ 8.85%), as drying and rehydration are associated with sample loss. Significant TBARS concentrations were obtained during lyophilization, dry storage for up to 72 h, and rehydration of RBCs specimens. The efficacy of the antioxidant tert-butyl hydroperoxide in reducing sample autoxidation during the TBARS assay is questionable due to its poor solubility in aqueous solution. Investigation of the interference caused by the 453-nm peak in the TBARS assay revealed that the origin of the peak is, at least in part, related to hydroperoxides. The data shows that the TBARS assay can be applied to desiccated RBC specimens. It also provides first evidence regarding the relationship between oxidative damage and desiccation. The modification of the MDA assay to dried cells will contribute to understanding the mechanism of damage that occurs during dehydration, dry storage and rehydration of dry biomaterials. 165

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Freeze-Drying of Red Blood Cells at Ultra-Low Temperatures

Cited by 44 publications

References 19 publications

Intracellular trehalose improves the survival of human red blood cells by freeze-drying

Intracellular trehalose improves the survival of human red blood cells by freeze-drying

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Determination of Lipid Peroxidation in Desiccated Red Blood Cells

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