Intracellular trehalose improves the survival of human red blood cells by freeze-drying

Abstract

Freeze-drying of human red blood cells has a potential important application for blood transfusion. The aim of this study was to investigate the effects of intracellular trehalose on the survival of red blood cells after freeze-drying and rehydration. Fresh red blood cells were incubated in trehalose solutions of various concentrations at 37°C for 7 h following freeze-drying. Polyvinylpyrrolidone, Trehalose, sodium citrate, and human serum albumin were used as extracellular protective agents for the freeze-drying of red blood cells. The results indicated that the intracellular trehalose concentration was increased with increasing concentration of extracellular trehalose solution, and the maximum concentration of intracellular trehalose reached 35 mmol/L. The viability of freeze-dried red blood cells increased with the increment of intracellular trehalose concentration.

Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

“…The intracellular trehalose concentrations observed in this study (Supplementary material) were low compared to literature [24,57]. Increase of the trehalose concentrations in the media resulted in cell death due to hyperosmotic conditions.…”

“…Freezing, drying and rehydration are stressful processes for biological structures and especially for living organisms. However, freeze-drying has been shown to preserve protein pharmaceuticals, vaccines, plasma, platelets, red blood cells and sperm cells [18,22,24,54,57] and it is a widely used method for drying heat sensitive and biological materials in general.…”

“…The importance of additive trehalose for preserving liposomes, red blood cells and platelets in freeze-drying is well established [14,24,57]. In anhydrobiotic organisms the amount of trehalose is typically high [9].…”

Synergistic Effect of Trehalose and Saccharose Pretreatment on Maintenance of Lyophilized Human Red Blood Cell Quality

“…The RBC osmotic fragility assay was performed according to a reported method [10]. After rehydration, RBC subsamples (20 μL) were separately added to varying concentrations of a 4.5 mL NaCl solution (2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, or 7 g/L).…”

Trehalose and Tween 80 Improve the Stability of Marine Lysozyme During Freeze-Drying

“…Freeze-drying was a commonly used stabilization technique for many proteins, yielding satisfactory products during longterm storage (11). Moreover, the decreased weight would favour its transportation and application.…”