Freezing and Freeze-Drying of Human Spermatozoa

Sherman, J.K.

doi:10.1016/s0015-0282(16)31685-5

Cited by 117 publications

(33 citation statements)

References 26 publications

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“…We demonstrated for the first time that EGTA solution, which is widely used in mice [2], [3], [4], [5], [6], [7], [8], [9], [10], [11], [12], [13] and [14], efficiently preserved the nucleus, acrosome and mitochondria of bovine sperm. In addition, we showed that presence of trehalose in medium supplement with FCS provided better protection to bovine sperm than the medium with FCS alone.…”

Section: Discussionmentioning

confidence: 99%

“…The first attempt to preserve mammalian sperm by dehydration was reported by Polge et al [3] using fowl sperm; after rehydration, the sperm were motile but their fertilizing ability was not evaluated. Subsequent efforts to lyophilize human and bovine sperm yielded very poor results [4] and [5]. In 1957, Yushchenko [6] reported for the first time the birth of a domestic animal following AI of freeze-dried rabbit sperm.…”

Section: Introductionmentioning

confidence: 99%

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Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

et al. 2007

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Freeze-drying sperm is an alternative to cryopreservation. Although sperm from various species has been freeze-dried, there are few reports for bovine sperm. The primary objective of this study was to evaluate the protective effect of various freeze-drying media on the structural and functional components of bovine sperm. The media tested were composed of TCM 199 with Hanks salts supplemented with 10% fetal calf serum (FCS) and TCM 199 with Hanks salts supplemented with 10% FCS and 0.2 M trehalose and EGTA solution. The efficiency of each medium on the preservation of freeze-dried sperm structures was evaluated with conventional and electron microscopy, DNA integrity was analyzed by a TUNEL assay, and fertilizing ability of lyophilized sperm was determined with ICSI. Although the plasma membrane was damaged in all media tested, mitochondria were similarly preserved in all freeze-drying treatments. The acrosome was best preserved in the media that contained trehalose (other treatments also conserved this structure). In contrast, media containing EGTA or trehalose most effectively preserved the nuclei in freeze-dried sperm, with only 2 and 5%, respectively, of cells with fragmented DNA. Furthermore, sperm conserved with these media also had higher (P < 0.05) rates of sperm head decondensation (32.5 and 27.5%), pronucleus formation (37.5 and 45.0%) and blastocyst formation (19.4 and 18.3%) than medium supplemented with FCS (15.0, 20.0 and 10.2%, respectively). In conclusion, media with EGTA and trehalose adequately protected bovine sperm during freeze-drying by preserving the viability of their nuclei.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

et al. 2007

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“…Perhaps the most exciting portion of this grant was the sperm desiccation studies with Sankha Bhowmick and Mehmet Toner. Methods for freeze-drying of human and bovine sperm were published in 1954-57 [28,29] and many studies on freeze-drying have been conducted since then. Mehmet and John's idea was to preserve sperm by desiccation at room temperature preferably, with long-term storage at room temperature or simple refrigeration.…”

Section: About the History Of The Culture Clubmentioning

confidence: 99%

A catalyst for change in reproductive science: John D. Biggers as a mentor’s mentor

Albertini

McGinnis

2013

J Assist Reprod Genet

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“…The idea of applying freeze-drying to sperm cells appeared as early as 1949 when it was reported that 50% of the FD fowl spermatozoa regained their motility after rehydration [8], followed by an another attempt in human spermatozoa [9]. Successful recovery of motile bull spermatozoa after freeze-drying and rehydration was first reported by Leidl [10] and was later reported by several groups [11][12][13][14].…”

Section: Research History Of Sperm Freeze-dryingmentioning

confidence: 99%

Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa

Hochi

Abdalla

Hara

et al. 2011

Journal of Reproduction and Development

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Abstract. Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species. Key words: Freeze-drying, Lyophilization, ICSI, Spermatozoa (J. Reprod. Dev. 57: [557][558][559][560][561][562][563] 2011) lthough spermatozoa from many mammalian species can be cryopreserved in a frozen status and used for offspring production either via artificial insemination (AI) or in vitro fertilization (IVF), freeze-drying (lyophilization), a widely used method for dehydrating a vast range of materials including foodstuffs, pharmaceuticals, biotechnology products, vaccines, diagnostics and biological materials, has been proposed as an alternative method for sperm preservation.Since freeze-dried (FD) spermatozoa after rehydration lose their motility, the essential requirement to complete physiological fertilization, a relatively difficult and specialized technique such as intracytoplasmic sperm injection (ICSI) must be applied to the FD spermatozoa. However, this tough working can overcome the potential disadvantages of sperm cryopreservation such as the high maintenance cost of frozen stock, the problems associated with transportation of frozen stock and the risk of accidental total loss of frozen stock. Advantages of sperm freeze-drying can be further emphasized when the spermatozoa from bulls with high economical importance are very sensitive to the process of cryopreservation (large variation in cryosensitivity among bulls) [1,2]. In species whose spermatozoa are difficult to preserve by freezing, the significance of freeze-drying will be magnified. Under the increasing demands for preservation of huge numbers of mutant or transgenic strains, the significance is also notable. In addition, freeze-drying offers advantages over conventional heat-drying by minimizing damage and loss of activity in delicate heat-labile materials such as enzymes, hormones and vaccines [3,4]. For parenteral products, the wet material can be accurately dispensed and sterilized by filtration just before filling into final containers, so the possibilities of particulation and bacterial contamination can be reduced [5]. The requirement of expensive equ...

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Freezing and Freeze-Drying of Human Spermatozoa

Cited by 117 publications

References 26 publications

Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm

A catalyst for change in reproductive science: John D. Biggers as a mentor’s mentor

Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa

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