Frequencies of background immunoglobulin-secreting cells in mice as a function of organ, age, and immune status

Benner, R.; Rijnbeek, Anne‐Marie; Bernabé, Rosa R.; Martínez-Alonso, Carlos; Coutinho, António

doi:10.1016/s0171-2985(81)80072-1

Cited by 56 publications

(26 citation statements)

References 9 publications

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“…In fact, throughout the whole life-span, a large fraction of TBL is pushed into terminal stages of differentiation (ten to one hundred times the splenic B lymphocytes), a very large proportion of them producing Tcell-dependent Ig isotypes. Present data differ substantially from those reported by Inaba et al [5], probably due to the low sensitivity of the developing antisera used by these authors since, not only in the case of thymic B cells but also when splenic B cells were tested for PFC, their numbers were rather low when compared with those in our study and in previous works [2]. We have also characterized the low responsiveness in vitro of TBL to LPS, which is due to a low frequency of LPS-responding B cell precursors as LD analysis shows and not to putative cell suppressive influences in bulk culture as recently suggested [5].…”

Section: Vh Gene Family Expression In Tbl Vs Splenic B Lymphocytescontrasting

confidence: 99%

“…We have previously reported the presence of considerable numbers of spontaneous Ab-secreting cells in the thymus of normal and germ-free mice [2].When compared to splenocytes, TBL showed a clear predominance of IgG and IgA with respect to IgM isotypes. Here we confirm and extend those results by an ontogenic and comparative analysis of immunologically distinct mice (Table 1).…”

Section: Quantitation Of Background Pfc In the Thymus: Analysis Throumentioning

confidence: 89%

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Ontogenic characterization of thymic B lymphocytes. Analysis in different mouse strains

et al. 1990

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We have characterized a population of murine B lymphocytes present in the thymus (TBL). They are a minor subset (0.2%-1% of total thymocytes), present from perinatal periods onwards and constituted by activated cells with a high proportion of Ig-secreting cells. They represent the first B lymphocytes detected that secrete IgG after birth. Functional analysis reveals that the frequency of lipopolysaccharide-responding cells in TBL is 5- to 10-fold lower than in the spleen. TBL from adult mice did not show any significant difference in their VH repertoire expression when compared to peripheral B lymphocytes. Furthermore, we have been able to isolate a subpopulation of B220+IgM-CD3- thymocytes whose putative B cell precursor potential needs to be directly analyzed. These and other findings support the intrathymic resident characteristics of TBL and suggest new ways of elucidating its physiological role in the complex selective processes occurring inside the thymus.

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Section: Vh Gene Family Expression In Tbl Vs Splenic B Lymphocytescontrasting

confidence: 99%

Section: Quantitation Of Background Pfc In the Thymus: Analysis Throumentioning

confidence: 89%

Ontogenic characterization of thymic B lymphocytes. Analysis in different mouse strains

et al. 1990

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“…However, another possible explanation would be that the localization of antibody-forming cells changes with increasing numbers of immunizations. The bone marrow has been sug gested to be an important site of antibody production, even after peroral antigen administration [2], How ever, preliminary results [Lycke et al, unpublished] have failed to demonstrate increasing numbers of anti body-forming cells in the bone marrow with repeated peroral booster immunizations with CT.…”

Section: Discussionmentioning

confidence: 99%

IgA Isotype Restriction in the Mucosal but Not in the Extramucosal Immune Response after Oral Immunizations with Cholera Toxin or Cholera B Subunit

Lycke¹,

Lindholm²,

Holmgren³

1983

Int Arch Allergy Immunol

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Intestinal mucosal as well as extramucosal antibody responses were studied in mice after peroral immunizations with cholera toxin or cholera B subunit. The immunizations with cholera toxin gave rise to a marked response with antitoxin-secreting cells (PFC) in Peyer’s patches (PP), mesenteric lymph nodes (MLN) and spleen showing isotype distribution of IgG > IgA > IgM and with PFC kinetics in MLN and spleen that suggested migration of cells from PP after peroral administration rather than cells stimulated in situ by adsorbed antigen. Highest numbers of PFC were obtained after 2 immunizations, and further administrations resulted in a decrease in the PFC response in MLN and spleen, while the PP responsiveness was relatively unchanged, and interestingly, protective immunity and IgA-dominated antitoxin titers in intestinal washings increased markedly by the additional boosters. Animals immunized with cholera B subunit, which lacks the adenylate cyclase-stimulating capacity of cholera toxin, showed similar PFC responses in extramucosal organs as those receiving cholera toxin but were poorly protected and had correspondingly lower IgA antitoxin titers in intestinal washings. These results suggest that the mucosal IgA antitoxin predominance is mainly due to regulatory mechanisms operating on the end-stage differentiation of the committed B cells in lamina propria and that this differentiation, as judged from the different results with cholera toxin and its B subunit, might be influenced by cyclic AMP.

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“…20 Thus, to determine changes of IgG and IgM ASCs in the spleen in the course of 20 weeks, we included age-matched control groups (naïve) for all time-points of this assessment.…”

Section: Immunohistochemical Detection Of Splenic Igg+ and Igm+ Ascsmentioning

confidence: 99%

Characterization of the Antibody Response after Cervical Spinal Cord Injury

Ulndreaj

Τζέκου

Mothe

et al. 2017

Journal of Neurotrauma

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The immune system plays a critical and complex role in the pathobiology of spinal cord injury (SCI), exerting both beneficial and detrimental effects. Increasing evidence suggests that there are injury level-dependent differences in the immune response to SCI. Patients with traumatic SCI have elevated levels of circulating autoantibodies against components of the central nervous system, but the role of these antibodies in SCI outcomes remains unknown. In rodent models of mid-thoracic SCI, antibody-mediated autoimmunity appears to be detrimental to recovery. However, whether autoantibodies against the spinal cord are generated following cervical SCI (cSCI), the most common level of injury in humans, remains undetermined. To address this knowledge gap, we investigated the antibody responses following cSCI in a rat model of injury. We found increased immunoglobulin G (IgG) and IgM antibodies in the spinal cord in the subacute phase of injury (2 weeks), but not in more chronic phases (10 and 20 weeks). At 2 weeks post-cSCI, antibodies were detected at the injury epicenter and co-localized with the astroglial scar and neurons of the ventral horn. These increased levels of antibodies corresponded with enhanced activation of immune responses in the spleen. Higher counts of antibodysecreting cells were observed in the spleen of injured rats. Further, increased levels of secreted IgG antibodies and enhanced proliferation of T-cells in splenocyte cultures from injured rats were found. These findings suggest the potential development of autoantibody responses following cSCI in the rat. The impact of the post-traumatic antibody responses on functional outcomes of cSCI is a critical topic that requires further investigation.

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Frequencies of background immunoglobulin-secreting cells in mice as a function of organ, age, and immune status

Cited by 56 publications

References 9 publications

Ontogenic characterization of thymic B lymphocytes. Analysis in different mouse strains

Ontogenic characterization of thymic B lymphocytes. Analysis in different mouse strains

IgA Isotype Restriction in the Mucosal but Not in the Extramucosal Immune Response after Oral Immunizations with Cholera Toxin or Cholera B Subunit

Characterization of the Antibody Response after Cervical Spinal Cord Injury

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