Frequency and genetic diversity of Echinococcus granulosus sensu stricto in sheep and cattle from the steppe region of Djelfa, Algeria

Laatamna, Abd Elkarim; Ebi, Dennis; Brahimi, Khadidja; Bediaf, Khadidja; Wassermann, Marion; Souttou, Karim; Römig, Thomas

doi:10.1007/s00436-018-6118-x

Cited by 21 publications

(18 citation statements)

References 33 publications

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“…(2017). The cyst isolated from the pelvis in this study (MH010305, ST8) was most similar to the haplotype EgAlg51 (belonging to the genotype G3 of the E. granulosus s.s. ) in the research conducted by Laatamna et al (2019).…”

Section: Resultsmentioning

confidence: 90%

“…The sequence of a cyst isolated from a patient's leg (MH010307, ST6) was identical to a cyst isolated from sheep in Portugal (HF947555) from genotype G1. Although the genotype of the armpit cyst (MH010306, ST7) was also G1, it differed in one nucleotide from a number of isolated hydatid cysts from sheep in Algeria (Laatamna et al , 2019), as well as one Tunisian isolate (KY766885) from the research conducted by Kinkar et al . (2017).…”

Section: Resultsmentioning

confidence: 90%

“…Over the recent years, several studies have been conducted to investigate the geographical distribution of genotypes of E. granulosus causing CE in human and animal hosts in different parts of the world (Cao et al , 2018; Laatamna et al ., 2019; Moudgil et al ., 2019); however, there is limited information regarding the relationship between parasite genotypes and their phenotypic characteristics, such as pathogenesis or the localization of their larva in unusual organs of the hosts.…”

Section: Introductionmentioning

confidence: 99%

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Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran

et al. 2019

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Despite several studies conducted to determine the genotypes of cystic echinococcosis (CE) agents in humans and other intermediate hosts, the relationship between parasite genotype and clinical presentation of hydatidosis is yet to be well defined. The aim of this study was to compare the genotypes/haplotypes of CE agents of human extra-hepatopulmonary hydatid cysts and common hydatid cysts of the liver. A comparative analysis was carried out between partial cox1 sequences of ten extra-hepatopulmonary hydatid cysts, two liver cysts and reliable sequences from the GenBank database. All the studied hydatid cysts had the Echinococcus granulosus sensu stricto (G1-G2-G3) genotypes. The liver CE cysts were caused by common G1 genotype, while six of the extra-hepatopulmonary cysts had genotypes different from common G1 cysts. The sequences of these six isolates were identical to the G2 and G3 genotypes of E. granulosus sensu stricto; the kidney and peritoneum cysts and most of the brain cysts were identified as G2 genotype, while G3 genotype was only reported in a cyst belonging to the pelvic region. Given the observed differences between the sequences of hydatid cysts, it seems that the replacement of hydatid cysts in organs other than the liver and lungs can be related to their genotypes and probably intra-genotypic characteristics. It was hypothesized that in each geographic area, less frequent genotypes were likely to be more consistent with placement in the host's unusual organs.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran

et al. 2019

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“…Originally Echinococcus genotyping relied on the analysis of a short fragment (366 bp) of the mt cox1 gene [9] that led to the identification of the E. granulosus s.s. G1-G3 genotypes. Over the past years, many studies on genetic diversity of E. granulosus have been based on the mtDNA sequences of the cox1 gene [3,[10][11][12][13][14], but it was not until recently [15,16] that the analysis of larger portions of mt genome evidenced how the cox1 gene could not clearly differentiate E. granulosus s.s. genotypes. A study published by Kinkar et al [17], based on a large dataset of near complete mitogenomes, evidenced instead, how a relatively short fragment of the nad5 gene could provide a consistent identification of E. granulosus s.s. G1 and G3 genotypes.…”

Section: Introductionmentioning

confidence: 99%

Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

et al. 2021

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Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

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“…The genetic diversity of E. granulosus s.s. at the intra-specific level is usually assessed by comparison of haplotypes from fragments, full lengths of mitochondrial genes (e.g., cox1 and nad1 ), or near-complete mitogenome sequences. Results have revealed high diversity of E. granulosus s.s. haplotypes in several parts of the world [ 11 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. Overall, all the studies have highlighted distribution of E. granulosus s.s. shaped by the spread of livestock domestication and intensive animal trade.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite Investigations of Multiple Echinococcus granulosus Sensu Stricto Cysts in Single Hosts Reveal Different Patterns of Infection Events between Livestock and Humans

M’rad

Oudni-M’rad

Bastid³

et al. 2020

Pathogens

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Cystic echinococcosis (CE) caused by the cestode Echinococcus granulosus sensu lato (s.l.) is a worldwide zoonosis and E. granulosus sensu stricto (s.s.) is the most common species associated with animal and human diseases. The objective of this study was to obtain a better understanding of CE infection in livestock and humans from very low and high endemic areas—France and Tunisia—by studying the genetic diversity of E. granulosus s.s. at the intra-individual host level. This genetic diversity was studied using EgSca6 and EgSca11 microsatellite profiles in 93 sheep from France and Tunisia, and in 12 cattle and 31 children from Tunisia only, all presenting multiple CE cysts (2 to 10 cysts). Overall, 96% of sheep, 92% of cattle, and 48% of children had at least two cysts with different microsatellite profiles. Inversely, 35% of sheep, 17% of cattle, and 65% of children had at least two cysts with the same microsatellite profile. The genotyping results for the CE samples highlight high and similar genetic diversity in France and Tunisia, suggesting that the probability of being successively infected by CE of the same microsatellite profile was rare in both countries. Therefore, our results suggest that in rare cases, several eggs of the same microsatellite profile, from two to seven in our data, can be ingested simultaneously in a single infection event and develop into several cysts in livestock and children. They also indicate that multiple infection events are frequent in livestock, even in a low endemic country such as France, and are less frequent but not negligible in children in a high endemic country such as Tunisia. Moreover, this is the first time that genetic evidence of secondary CE has been found. Further studies are needed to better assess the pattern of infection events in livestock and humans, especially by studying the genetic diversity of adult worms in definitive hosts.

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Frequency and genetic diversity of Echinococcus granulosus sensu stricto in sheep and cattle from the steppe region of Djelfa, Algeria

Cited by 21 publications

References 33 publications

Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran

Genotyping and phylogenetic analysis of unusually located hydatid cysts isolated from humans in north-east Iran

Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Microsatellite Investigations of Multiple Echinococcus granulosus Sensu Stricto Cysts in Single Hosts Reveal Different Patterns of Infection Events between Livestock and Humans

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