Frequency and Types of Point Mutation at the 12th Codon of the c‐Ki‐<i>ras</i> Gene Found in Pancreatic Cancers from Japanese Patients

Mariyama, Mariko; Kishi, Kiyozo; Nakamura, Keikichi; Obata, Hiroshi; Nishimura, Susumu

doi:10.1111/j.1349-7006.1989.tb01687.x

Cited by 109 publications

(51 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The narrow spectrum of mutation (GGT to GAT or GTT) in BTCs is noteworthy compared with other types of malignancies such as pancreatic or colon cancer (Forrester et al, 1987;Mariyama et al, 1989;Scarpa et al, 1994). The method used in the present study for detecting mutation is well established and is generally considered reliable for detecting mutations (Orita et al, 1989), so a technical basis for the findings seems unlikely.…”

Section: Discussionmentioning

confidence: 87%

Ki-ras point mutations and proliferation activity in biliary tract carcinomas

Ohashi

Tstsumi²,

Nakajima³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

Summary The association between Ki-ras mutations and proliferation activity was investigated in a comprehensive series of biliary tract carcinomas (BTCs). We precisely microdissected samples of tissue from paraffin-embedded sections of 77 BTCs including 22 intrahepatic cholangiocarcinomas (ICCs), 36 extrahepatic cholangiocarcinomas (ECCs), and 19 gall bladder carcinomas (GBCs). Ki-ras mutations at exons 1 and 2 were determined by the polymerase chain reaction -single strand conformation polymorphism (PCR-SSCP) method and confirmed by direct sequencing. Proliferation activity was immunohistochemically assessed to generate proliferating cell nuclear antigen labelling indices (PCNA LIs). Ki-ras mutations were detected in 10 of 22 ICCs (45%), 24 of 36 ECCs (67%), and in 16 of 19 GBCs (84%). The frequency of Ki-ras mutations in peripheral type ICCs was 33% (4 of 12) and that in the hilar type ICCs was 60% (6 of 10). In ECCs the highest value of 82% (9 of 11) was found for carcinomas located in the lower part of the biliary tree. Mean PCNA LI in mutation-positive BTCs was significantly elevated compared with the mutation-negative value. These results indicate frequent involvement of Ki-ras mutations in BTCs, especially in GBCs and in distal ECCs, and that carcinomas harbouring a mutation feature high cell proliferation activity.

show abstract

Section: Discussionmentioning

confidence: 87%

Ki-ras point mutations and proliferation activity in biliary tract carcinomas

Ohashi

Tstsumi²,

Nakajima³

et al. 1996

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Single base substitutions can be readily detected by a number of methods, including allelic-specific oligonucleotide (ASO) hybridization (18,19), RNAse A mismatch cleavage of the DNA-RNA hybrids (20,21), or DNA sequencing, either directly (22,23) or after cloning the PCR products in plasmids (24,25). The generation or destruction of restriction endonuclease sites (RFLP) by point mutations also allows their rapid detection after the genomic sequences have been amplified by the PCR (26,27 (29).…”

Section: Methodsmentioning

confidence: 99%

Frequency and Spectrum of Mutations at Codons 12 and 13 of the C-K-ras Gene in Human Tumors

Capellà¹,

Cronauer-Mitra²,

Peinado³

et al. 1991

Environmental Health Perspectives

View full text Add to dashboard Cite

The frequency of point mutations at codons 12 and 13 of the c-K-ras gene has been determined in a panel of more than 400 human tumors. Mutant c-K-ras genes were detected in about 75% of adenocarcinomas of the pancreas (n = 84); 40% of adenomas (n = 72) and carcinomas (n = 244) of the colon and rectum; 30% of carcinomas of the bile duct (n = 19); 25% of carcinomas of the lung (n = 92), and in lower frequency in other carcinomas, including liver, stomach, and kidney. No mutations were found in carcinomas of the breast, prostate, esophagus, and gall bladder, among others. Comparative analysis of the spectrum of mutations show that while G to A transitions were the most frequent mutations in pancreatic and colo-rectal tumors, G to T transversions were more prevalent in lung carcinomas. The aspartic acid mutation at codon 13 (GGC -* GAC) was relatively frequent in colo-rectal tumors but rare in pancreatic and lung carcinomas. The differences in the mutation spectrum of the c-K-ras gene in cancers of the gastrointestinal and respiratory tracts are suggestive of differential exposure to genotoxic agents.

show abstract

“…For example, ras mutations are detected in 40-50% of colon carcinomas (Perucho et al, 1981;Vogelstein et al, 1988;Burmer et al, 1991;Wang et al, 1993); 80% of pancreatic carcinomas (Mariyama et al, 1989;Shibata et al, 1990;Burmer et al, 1991;Pinto et al, 1997); and 30-50% of lung adenocarcinomas (Rodenhuis et al, 1988;Suzuki et al, 1990, Reynolds et al, 1991Li et al, 1994;Mills et al, 1995). Kras2 mutations account for >90% of the activating ras mutations observed in these tumor types.…”

Section: Introductionmentioning

confidence: 99%

LOH of chromosome 12p correlates with Kras2 mutation in non-small cell lung cancer

Zhang

Dai

et al. 2003

Oncogene

View full text Add to dashboard Cite

Previous observation has shown that the wild-type Kras2 allele is a suppressor of lung cancer in mice. Here we report that loss of heterozygosity (LOH) of chromosome 12p was detected in B50% of human lung adenocarcinomas and large cell carcinomas, and Kras2 mutations were detected at codon 12 in B40% of adenocarcinomas and large cell carcinomas. Interestingly, all of the lung adenocarcinomas and large cell carcinomas containing a Kras2 mutation exhibited allelic loss of the wild-type Kras2 allele when a correlation between LOH of the region on chromosome 12p and Kras2 mutation was made. These results from human lung cancer tissues provide a strong evidence in support of our previous observation in mouse models that the wild-type Kras2 is a tumor suppressor of lung cancer.

show abstract

Frequency and Types of Point Mutation at the 12th Codon of the c‐Ki‐ras Gene Found in Pancreatic Cancers from Japanese Patients

Cited by 109 publications

References 20 publications

Ki-ras point mutations and proliferation activity in biliary tract carcinomas

Ki-ras point mutations and proliferation activity in biliary tract carcinomas

Frequency and Spectrum of Mutations at Codons 12 and 13 of the C-K-ras Gene in Human Tumors

LOH of chromosome 12p correlates with Kras2 mutation in non-small cell lung cancer

Contact Info

Product

Resources

About