Frequency of Aspergillus fumigatus fres.: A toxigenic and allergenic fungal species in milking cows feeds throughout one research year

This paper presents the results of an investigation related to mycological populations of rapeseed samples produced in the Institute of Field and Vegetable Crops in Novi Sad (location: Rimski Sancevi, Novi Sad), with a special emphasis on the potentially toxigenic mycopopulations. Mycological investigations were performed on the samples that were treated with 4% solution of Na-hypochlorite, and on the ones that were not submitted to this treatment. Isolation and determination of total mould count was carried out using Dichloran Glycerol Agar (DG18). The identification of isolated moulds was done according to modern keys for fungal determination. From 20 untreated tested samples, 17 were contaminated with moulds (10.0 to 4.7x102 cfu/g). When the samples were treated with 4% solution of Na-hypochlorite, moulds were isolated only form 4 samples, and the total mould count ranged from 10.0 to 60.0 cfu/g. In the isolated mycopopulations, xerophilic moulds dominated, especially those from the genera Aspergillus, Eurotium and Penicillium. In the isolated mycopopulations, high degree of isolated species belonged to toxigenic species from the genera Alternaria, Aspergillus, Fusarium, Eurotium and Penicillium.

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Xerophilic mycopopulations isolated from rapeseeds (Brassica napus)

Skrinjar

Miklic

Kocic-Tanackov

et al. 2013

ACTA PERIOD TECHN

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Results of one-year investigations of the contamination of dairy cattle feed and raw milk with moulds and mycotoxins

Skrinjar

Jakic-Dimic²,

Blagojev

et al. 2011

BIOTEC ANIM HUSB

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The contamination of 98 dairy cattle feed samples with moulds and mycotoxins (AB1, OTA, ZEA) was investigated. The contamination of 80 raw milk samples with aflatoxin M1 was also tested. Determination of total viable counts per 1g of feed was performed using Koch's standard method. The content of AB1, OTA and ZEA in feed, as well as aflatoxin M1 in raw milk samples, was determined by VICAM fluorimetric method. Results showed that 91% of all tested samples was contaminated with moulds, and total viable mould count per gram ranged from 10 (corn silage-spring) to 4.9 x 10 6 per gram (dried alfalfa-winter). The isolated moulds belong to 20 genera and 72 species. Results of mycotoxicological investigations showed that 42 samples of total 98 tested, were contaminated with mycotoxins. The most frequent was OTA (24 samples, 20.00-210.00 µg/kg) followed with ZEA (12 samples, 250.00-980.00 µg/kg) and AB1 (3 samples, traces-18.50 µg/kg). Aflatoxin M1 in raw milk samples wasn't found.

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Investigation of dissemination of aspergillosis in poultry and possible control measures

Kapetanov

Potkonjak

Milanov

et al. 2011

Zb Mat srp prir nauk

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Fungi belonging to genus Aspegillus are ubiquitous saprophytic microorganisms which are, in certain circumstances, responsible for clinical infections of respiratory tract in all poultry, particularly in young birds. In case of a lung form, Aspergillus fumigatus, A. niger and A. glaucus are the most frequently isolated fungi. In general, poultry is constantly exposed to these fungi in its environment. Predisposing factors, such as long exposition and highly contaminated environment and litter, high humidity in poultry houses, poor ventilation, malnutrition and stress, all contribute to clinical aspergillosis. Some geographic and seasonal regularities are observed in relation to the distribution of disease outbreaks. In this sense, cases of aspergillosis in our country were more frequently noted in wild areas located northern from the rivers Sava and Danube. Influence of some factors on the outbreak and spreading, as well as predominant clinical features of aspergillosis in poultry were investigated in this paper. Possible prophylactic and intervention measures were discussed. The occurrence of Aspergillus sp. in poultry was analyzed according to the clinical and laboratory investigations performed during the two selected years, 2000 and 2010. Widespread aspergillosis was noted in poultry flocks of different age, both in young and adult birds. During the years 2000 and 2010, acute aspergillosis was found in 12 and 16 commercial flocks of chickens and turkeys, respectively. Ocular infection with Aspergillus was determined in 10 day old broilers from two flocks. Aspergillus sp. was isolated from unhatched eggs (6.86%), litter (23.07%), environmental (36.17%) and hatchery swabs (3.85%). Besides the appropriate antifungal therapy, enforcement of proper sanitary-hygiene measures on poultry farms and hatcheries, as well as microbiological control of feed are considered essential for an efficient control of infection and its spreading

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Frequency of Aspergillus fumigatus fres.: A toxigenic and allergenic fungal species in milking cows feeds throughout one research year

Cited by 3 publications

References 10 publications

Xerophilic mycopopulations isolated from rapeseeds (Brassica napus)

Xerophilic mycopopulations isolated from rapeseeds (Brassica napus)

Results of one-year investigations of the contamination of dairy cattle feed and raw milk with moulds and mycotoxins

Investigation of dissemination of aspergillosis in poultry and possible control measures

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