Frequency of BMP15 and GDF9 mutations increasing litter size and their phenotypic effects in Olkuska sheep population

Abstract

Two mutations affecting the ovulation rate and litter size are segregating in Olkuska sheep population, FecXO in the BMP15 gene, and the G7 site mutation in GDF9 gene. Homozygous carriers of both mutations are hyperprolific, contrary to the sterility observed in homozygous carriers of most other BMP15 and GDF9 mutations. The objective of this study was to assess frequency and phenotypic effects of both mutations. Blood samples were obtained from 740 individuals, 111 rams and 629 ewes, out of which 91 rams and 561 ewes were successfully genotyped for the BMP15 and GDF9 loci. The reproductive performance included a number of lambs born/born alive and a number of lambs reared until 60 days of age, and for a subset of ewe ovulation rates. The study proved a high frequency of the FecXO mutation in two flocks that have been selected for many years for increased litter size (0.7 - 1.0 in breeding ewes and rams respectively), and a moderate frequency in another 19 private flocks (0.4 - 0.5). The frequency of the GDF9 mutation was low, with only 50 sheep out of 312 genotyped being carriers of the GDF9/G7 mutation, including three homozygous carriers. The FecXO mutation in the BMP15 had a significant effect on both litter size and the ovulation rate. The single copy in heterozygous carriers increased litter size by 0.255 (0.063), while the effect of two copies in homozygous genotypes was + 0.874 (0.081) lambs born. Due to the low frequency of the GDF9 mutation, it can only be preliminary concluded that litter size has been increased in double carries, both of the BM15 and GDF9 mutation, which may suggest their additive interaction. The positive effect of both mutations supports their direct use in selection programmes.

Detection of Novel Variations Related to Litter Size in BMP15 Gene of Luzhong Mutton Sheep (Ovis aries)

“…In this study, the geographic distribution of allele frequencies for SNPs c.352+342C>A and c.352+1232T>C in BMP15 indicated that Western Europe has the highest variation frequency for these two SNPs, although the detailed worldwide distributions of the allele frequencies are distinct for the two variations. This result coincides with reports on polymorphisms in this gene showing that its variations mainly occur in European sheep [ 4 , 5 , 9 , 10 , 11 , 12 , 13 , 28 , 29 , 32 , 34 , 35 , 36 ], which implies that BMP15 is a key gene for high litter size of European sheep.…”