Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

Huescas, C. G. Y.; Pereira, Rebeca Inhoque; Prichula, Janira; Azevedo, Pedro Alves d'; Frazzon, Jeverson; Frazzon, Ana Paula Guedes

doi:10.1590/1519-6984.183375

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…In this study, the prevalence of CRISPR1 was higher in E. faecalis (50.0%) than E. faecium isolates (28.6%) that was similar to Lyons et al 31 In the current study, the frequency of CRISPR-Cas systems was significantly higher in E. faecalis (80.0%) than E. faecium (40.0%). This finding was in line with a previous report by Huescas et al 29 from Brazil, who found that the CRISPR-Cas elements of E. faecalis were detected more often than those of E. faecium in non-clinical samples. However, they reported more and less prevalence of CRISPR2 and CRISPR3-cas in the E. faecalis and E. faecium isolates from food and animal fecal samples than in our study, respectively.…”

Section: Discussionsupporting

confidence: 93%

“…A previous study by Dos Santos et al, 14 showed a lower frequency rate (51.1%) of CRISPR-Cas in enterococci collected from human and food sources. Using PCR, the frequency of CRISPR2 (55.3%) was higher than CRISPR1 (41.2%) and CRISPR3 (35.3%) in this study which was in line with previous reports by Gholizadeh et al 11 from Iran and Huescas et al 29 from Brazil. In contrast to our results, Dos Santos et al 14 reported CRISPR3 (67.4%) as the most prevalent type in enterococci isolates.…”

Section: Discussionsupporting

confidence: 93%

“… 32 Also, this research showed the prevalence of 28.6%, 22.9%, and 4.3% for CRISPR1, CRISPR2, and CRISPR3 in E. faecium isolates, respectively. In this regard, Huescas et al 29 reported a higher prevalence rate of CRISPR2 (32.7%), but lower frequency of CRISPR1-cas (5.6%) and CRISPR3-cas (1.8%) genes in non-clinical E. faecium isolates. These discrepancies in the results of various studies can be due to the sample size, the source of the sample collection, and the technique used in the screening of CRISPR-Cas systems.…”

Section: Discussionmentioning

confidence: 97%

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Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance in Enterococcus faecalis and Enterococcus faecium Collected from Hospital Wastewater

Alduhaidhawi¹,

AlHuchaimi²,

Al-Mayah³

et al. 2022

IDR

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Purpose This study aimed to evaluate the presence of CRISPR-Cas system genes and their possible association with antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium species isolated from hospital wastewater (HWW) samples of several hospitals. Methods HWW samples (200 mL) were collected from wastewater discharged from different hospitals from October 2020 to March 2021. The isolation and identification of enterococci species were performed by standard bacteriology tests and polymerase chain reaction (PCR). Antibiotic resistance was determined using the disc diffusion. The presence of various CRISPR-Cas systems was investigated by PCR. The association of the occurrence of CRISPR-Cas systems with antibiotic resistance was analyzed with appropriate statistical tests. Results In total, 85 different enterococci species were isolated and identified using phenotypic methods. The results of PCR confirmed the prevalence of 50 (58.8%) E. faecalis and 35 (41.2%) E. faecium , respectively. In total, 54 (63.5%) of 85 isolates showed the presence of CRISPR-Cas loci. The incidence of CRISPR-Cas was more common in E. faecalis . CRISPR1, CRISPR2, and CRISPR3 were present in 35 (41.2%), 47 (55.3%), and 30 (35.3%) enterococci isolates, respectively. The CRISPR-Cas positive isolates showed significant lower resistance rates against vancomycin, ampicillin, chloramphenicol, erythromycin, rifampin, teicoplanin, tetracycline, imipenem, tigecycline, and trimethoprim-sulfamethoxazole in comparison with CRISPR-Cas negative isolates. The results showed that the presence of CRISPR-Cas genes was lower in multidrug-resistant (MDR) isolates (53.1%, n = 26/49) compared to the non-MDR enterococci isolates (77.8%, n = 28/36) ( P = 0.023). Conclusion This study revealed the higher prevalence of E. faecalis than E. faecium in HWWs. Also, the lack of CRISPR-Cas genes was associated with more antibiotic resistance rates and multidrug resistance in E. faecalis and E. faecium isolates with HWW origin.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance in Enterococcus faecalis and Enterococcus faecium Collected from Hospital Wastewater

Alduhaidhawi¹,

AlHuchaimi²,

Al-Mayah³

et al. 2022

IDR

View full text Add to dashboard Cite

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“…In the current study, we found that the presence of orphan CRISPR2(42%) was more in Enterococcus than CRISPR1-Cas (23%) and CRISPR3-Cas (5%), which was similar to Alduhaidhawi. 28 In non-clinical Enterococcus faecium isolates, Huescas et al 29 demonstrated a higher prevalence rate of CRISPR2 and lower frequency of CRISPR1-Cas (5.6%) and CRISPR3-Cas (1.8%) system. In contrast to our study, Palmer et al 30 found that CRISPR2 was present in all Enterococcus faecalis isolates.…”

Section: Discussionmentioning

confidence: 99%

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Tao¹,

Chen²,

Li³

et al. 2022

IDR

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Purpose This study aimed to investigate the prevalence of the CRISPR-Cas system in nosocomial isolates of Enterococcus and their possible association with antibiotic resistance and virulence genes. Materials and Methods Identification and antimicrobial susceptibility of the microorganism were performed by the automatized VITEK 2 Compact system (bioMerieux, France). A total of 100 Enterococcus isolates were collected and identified by VITEK 2 Compact automatic microbial identification drug susceptibility analyzer. The prevalence of various CRISPR-Cas systems, antibiotic resistance genes and virulence genes were investigated by polymerase chain reaction (PCR). The prevalence of CRISPR-Cas systems associated with antibiotic resistance and virulence genes was performed by appropriate statistical tests. Results A total of 100 isolates of Enterococcus were identified and there were 62/100(62.0%) Enterococcus faecalis isolates and 38/100(38.0%) Enterococcus faecalis isolates. In total, 46 (46.0%) of 100 isolates had at least one CRISPR-Cas locus. CRISPR elements were more prevalent in Enterococcus faecalis isolates. The results of PCR demonstrated that CRISPR1-Cas, orphan CRISPR2, and CRISPR3-Cas were present in 23 (23.0%), 42 (42.0%) and 5 (5.0%) Enterococcus isolates, respectively. Compared with CRISPR-Casnegative isolates, the CRISPR-Cas positive isolates showed significant lower resistance rates against ampicillin, erythromycin, levofloxacin, tetracycline, vancomycin, gentamicin, streptomycin, and rifampicin. Presumably consistent with drug susceptibility, fewer CRISPR loci were identified in vanA, tetM, ermB, aac6’-aph(2”), aadE , and ant(6) positive isolates. There was a significant negative correlation between the CRISPR-Cas locus and the enterococcal virulence factors enterococcal surface protein ( esp ) gene. Conclusion In conclusion, the results indicated that the absence of the CRISPR-Cas system was negatively associated with some antibiotic resistance in clinical isolates of Enterococcus faecalis and Enterococcus faecium . Also, there was a negative correlation with the carriage of antibiotic resistance genes. Furthermore, CRISPR-Cas may prevent some isolates from acquiring certain virulence factors.

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“…This complex array signifies a favorable evolutionary adaptation to a symbiotic lifestyle and reflects a potent ability to resist phage attacks within the sponge’s ecosystem. Interestingly, CRISPR/Cas systems have also been reported from samples of feces in wild marine animals and the deep-sea hagfish gut [ 110 , 111 ].…”

Section: Huge Inventory Of Crispr/cas In the Oceanmentioning

confidence: 99%

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

Li,

Wu,

Zhang

et al. 2024

Microorganisms

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Initially discovered over 35 years ago in the bacterium Escherichia coli as a defense system against invasion of viral (or other exogenous) DNA into the genome, CRISPR/Cas has ushered in a new era of functional genetics and served as a versatile genetic tool in all branches of life science. CRISPR/Cas has revolutionized the methodology of gene knockout with simplicity and rapidity, but it is also powerful for gene knock-in and gene modification. In the field of marine biology and ecology, this tool has been instrumental in the functional characterization of ‘dark’ genes and the documentation of the functional differentiation of gene paralogs. Powerful as it is, challenges exist that have hindered the advances in functional genetics in some important lineages. This review examines the status of applications of CRISPR/Cas in marine research and assesses the prospect of quickly expanding the deployment of this powerful tool to address the myriad fundamental marine biology and biological oceanography questions.

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Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

Cited by 6 publications

References 28 publications

Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance in Enterococcus faecalis and Enterococcus faecium Collected from Hospital Wastewater

Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance in Enterococcus faecalis and Enterococcus faecium Collected from Hospital Wastewater

Association of CRISPR-Cas System with the Antibiotic Resistance and Virulence Genes in Nosocomial Isolates of Enterococcus

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

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