Frequency of HLA antibodies in south India

Pitchappan, Ramasamy; Agilan, S.; Mahendran, V.; Brahmajothi, V.; Kumar, Uday; Balakrishnan, K.; Kumari, Rekha; Sashidhar, M.; SamalRaj,; Manuel, May; Raja, V. Indra

doi:10.1007/bf02702995

Cited by 4 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Values range from 7.3% to 36% (7.3%, Swedish women;[ 9 ] 18.7%, Caucasian women;[ 10 ] 21.6%, American women;[ 11 ] 29%, Mestizo women[ 11 ] and 9.6%, Warao women;[ 11 ] Venezulean women;[ 12 ] 36% USA women[ 13 ] and south Indian women 10.6%. [ 14 ] Another study detected anti-HLA antibodies in only 5% of women with successful pregnancies during the first trimester, compared with an antibody prevalence of 10% in women who miscarried. [ 15 ] In a prospective immunization study, Christiansen et al .…”

Section: Discussionmentioning

confidence: 99%

Role of anti-human lymphocyte culture cytotoxic antibodies in recurrent spontaneous pregnancy loss women

Shankarkumar

Ramrakhiyani

et al. 2011

J Hum Reprod Sci

View full text Add to dashboard Cite

BACKGROUND:Recurrent spontaneous pregnancy (RSA) is defined as a sequence of three or more consecutive spontaneous abortions. One of the major causes of RSA is immunological where alloimmune antibodies develop towards human leucocyte antigen (HLA) antigens. Earlier research had suggested that anti-HLA antibodies are produced in normal women; studies have been reported that normal pregnant women develop anti-HLA antibodies, mostly after 20–28 weeks of gestation.AIM:To evaluate the role of anti-HLA antibodies in RSA patientsMATERIALS AND METHODS:A total of 80 randomly selected couples with unexplained three or more RSA and control group of 50 normal pregnant women were screened for anti-HLA A and B antibodies. The anti-HLA antibodies were analyzed following the standard two-stage NIH microlymphocytotoxicity assay.RESULTS:In our study group a high frequency of anti-HLA antibodies among women with RSA (26.25%) was detected compared to normal pregnant women (8.0%). Most of the sera showed HLA-A and HLA-B antibodies which had high titer, up to a dilution of 1: 4096.CONCLUSION:This incidence of high anti-HLA antibodies in RSA women during early weeks of gestation may explain the recurrent pregnancy loss.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of anti-human lymphocyte culture cytotoxic antibodies in recurrent spontaneous pregnancy loss women

Shankarkumar

Ramrakhiyani

et al. 2011

J Hum Reprod Sci

View full text Add to dashboard Cite

show abstract

“…11,12 A total of 190 indigenous antisera were used for defining 17 specificities for HLA-A locus, 29 for HLA-B locus, 8 for HLA-C locus, and 10 for HLA-DR locus antigens. 13 The typing tray included a minimum of 3 antisera for each supertype specificity.…”

Section: Methodsmentioning

confidence: 99%

Human Leukocyte Antigens in Hypertrophic Cardiomyopathy Patients in South India

Shankarkumar

Pitchappan

Pethaperumal

2004

Asian Cardiovasc Thorac Ann

View full text Add to dashboard Cite

Hypertrophic cardiomyopathy is characterized by massive ventricular hypertrophy, reduced diastolic function, and excessive ventricular contraction. The human leukocyte antigens HLA-A, HLA-B, and HLA-DR were studied in 14 hypertrophic cardiomyopathy patients with left ventricular obstruction from South India. They were compared with 81 normal age- and sex-matched individuals from the same ethnic background. The human leucocyte antigens were identified using the standard serological assay with a longer incubation for DR antigens. The odds ratio, frequency, chi-squared value, p-value, etiological fraction, preventive fraction, and haplotype frequency estimates were calculated. The HLA-B51 and HLA-DR2 levels were significantly increased in hypertrophic cardiomyopathy patients compared to controls, whereas HLA-A19, HLA-B7, and HLA-DR4 were decreased when compared to the controls. It was noticed that haplotype B51-DR2-DQ3 was significantly associated with hypertrophic cardiomyopathy patients from South India. Hypertrophic cardiomyopathy may be associated with genes in the human leukocyte antigen region, and immunogenetic factors linked to human leukocyte antigens appear to play a major role in the pathogenesis.

show abstract