Frequency of mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from Sudan

Elbir, Haitham; Ibrahim, Nuha

doi:10.3855/jidc.4496

Cited by 8 publications

(18 citation statements)

References 13 publications

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“…15,16 The occurrence of 531 mutations (11.7%) in this study was comparable to 9.4% found in the previous study. 10 Our finding and those of Burkina Faso neighboring countries 11,[12][13][14] contrast enormously with the results of numerous studies [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] which reported higher rates of mutations at 531. High rates of 531 mutations were particularly found in Kyrgyzstan, Ethiopia, Brasil, South West Ethiopia, Georgia, Taiwan and Samara in Russia.…”

Section: Discussioncontrasting

confidence: 98%

“…It corroborated with similar reports from elsewhere. 17,36,37 It is possible that less common mutations in rpoB gene cannot be detected by DRplus. 21 In addition, the possibilities of absence of mutation in RRDR of rpoB gene in MDR-TB isolates may be due to existence of other rare rpoB mutations outside RRDR or different mechanism of rifampicin resistance.…”

Section: Discussionmentioning

confidence: 99%

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Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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Genetic mutations are responsible for the high rate of resistance observed in the treatment of tuberculosis. This study aimed at determining the occurrence of mutations associated with rifampicin (RIF) and isoniazid (INH) resistance of Mycobacterium tuberculosis complex (MTBC) isolates. MTBC strains isolated by culture from 110 TB patients diagnosed with resistant to rifampicin (RR-TB) by Xpert MTB/RIF were studied. The isolates were obtained from the National Tuberculosis Reference Laboratory in Ouagadougou. They were identified culturally using Antigenic method (SD Bioline TB Ag MPT64). Polymerase Chain Reaction, PCR (DRplus) was used to detect the occurrence of mutations in the genes associated with resistance katG and inhA promoter for INH, and rpoB for RIF. Out of 103 isolates with RIF resistant, mutations were detected in 87(84.5%) of gene rpoB while no mutation was found in 16(15.5%) of the gene of the isolates even though the wild probes had disappeared. Single mutations were found in the codons D516V (41.7%) and H526Y (17.5%) while combined mutations (single and double) were mostly detected in the codons D516 (51.5%), H526Y (20.4%), S531L (11.7%) and H526D (10.7%) respectively. Single mutations responsible for high-level isoniazid resistance, katG were observed in the codon S315T1 while the combined inhA and katG were detected in the codon C8T and S315T, 16 (14.5%) respectively. The highest mutation occurrence was observed with rpoB516, rpoB526 for RIF and katG315 for INH associated with resistance of MTBC isolates. There is a need to improve molecular assay kit diagnosis to curb the geographic specificity of the target genes needed to detect more possible mutations.

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Diandé¹,

Ogbomon²,

Gueye³

et al. 2019

IJMBOA

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“…Several previous studies have reported vastly variable frequencies of these mutations; with katG315 gene mutations accounting for 42 to 95% and mutation in the inhA-15 promotor region accounting for 6 to 43% of phenotypic INH resistance [12,16,17,22,23]. that the frequency of gene mutation at codon 531, 526 and 516 were 64.1%, 17.9%, and 7.7%, respectively [9]. The same repot revealed that all genetic alterations were occurred by single base substitutions, and the most common mutation was observed at codon S531L [9].…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, a study conducted by Elbir et al . from Sudan reported that the frequency of gene mutation at codon 531, 526 and 516 were 64.1%, 17.9%, and 7.7%, respectively [9]. The same repot revealed that all genetic alterations were occurred by single base substitutions, and the most common mutation was observed at codon S531L [9].…”

Section: Discussionmentioning

confidence: 99%

“…During the past decade, several molecular (genotypic) DST methods which can detect gene mutations that confer drug resistance have been developed, including line probe assays, real-time polymerase chain reaction (PCR), deoxyribonucleic acid (DNA) sequencing, DNA hybridization on designed chips, and pyrosequencing [3,[8][9][10]. In pursuance of these molecular diagnostic approaches to correctly identify all resistant Mtb, the genes and specific nucleotide change conferring antitubercular drug resistance should be known and included in the diagnostic test.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of drug resistance-conferring mutations associated with isoniazid and rifampicin resistantMycobacterium tuberculosisin Ethiopia: A systematic review and meta-analysis

Reta

Alemnew

Abate

2020

Preprint

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Background: Drug resistance tuberculosis (DR-TB) continues to be a major public health threat globally. Due to the development of many rapid molecular diagnostic tools to detect gene mutations in M.tuberculosis (Mtb), specific genes conferring resistance to different anti-TB drugs have been identified. The aim of this meta-analysis was to assess the prevalence of the gene mutations associated with rifampicin (RIF) and isoniazid (INH) resistant Mtb in Ethiopia. Methods: Using PRISMA guideline, we systematically searched a literature on PubMed/MEDLINE, Web of Science, Scopus electronic databases, Cochrane library, and other database sources. The data analysis was done using STATA 11. The pooled prevalence of the gene mutations associated with resistance to RIF and INH were estimated using the random effect model. Heterogeneity was measured by the I2 statistical test, and the publication bias through the funnel plot and the Eggers regression test. Results: Among all antimycobacterial resistance tested TB patients, prevalence of resistance to any anti-TB drug was 31.3%, while multidrug resistance TB (MDR-TB), any RIF and INH resistance were 22.2%, 24.9%, and 27.9%, respectively. In total, 909 (95.8%) of 949 INH resistant Mtb isolates had detectable gene mutation in katG315 and 5.9% in the inhA gene. The meta-analysis derived an estimated pooled prevalence of katGMUT1(S315T1) in INH resistant Mtb was 89.18% (95%CI 81.94-96.43%), while a pooled inhAMUT1 (C15T) resistant Mtb prevalence was 77.48% (95% CI 57.84-97.13%). Besides, 769 (90.8%) of 847 RIF resistant strains had detectable rpoB gene mutation, commonly in rpoBMUT3(S531L) probe (550 cases). The meta-analysis resulting a pooled rpoBMUT3(S531L) resistant Mtb prevalence of 74.20 % (95%CI 66.39-82.00%). Conclusions: RIF resistant Mtb isolates were spread widely, mainly with S531L mutation. Similarly, INH resistant Mtb isolates were spread with S315T1 and C15T mutations. It is significant to detect S531L among RIF resistant and S315T1 and C15T mutations among INH resistant isolates as it may be a determinant for subsequent development of MDR-TB. Rapid diagnosis of RIF and INH resistant Mtb strains in TB patients would expedite modification of treatment regimens, and proper infection control interventions could be taken on time to reduce the risk of further development and transmission of MDR-TB.

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Prevalence of drug resistance-conferring mutations associated with isoniazid- and rifampicin-resistant Mycobacterium tuberculosis in Ethiopia: a systematic review and meta-analysis

Reta

Alemnew

Abate

et al. 2021

Journal of Global Antimicrobial Resistance

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Frequency of mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from Sudan

Abstract: This item has no abstract, Follow the links below to access the full text.

Cited by 8 publications

References 13 publications

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Occurrence of mutations associated with rifampicin and isoniazid resistant in Mycobacterium tuberculosis isolates from patients in Burkina Faso

Prevalence of drug resistance-conferring mutations associated with isoniazid and rifampicin resistantMycobacterium tuberculosisin Ethiopia: A systematic review and meta-analysis

Prevalence of drug resistance-conferring mutations associated with isoniazid- and rifampicin-resistant Mycobacterium tuberculosis in Ethiopia: a systematic review and meta-analysis

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