Frequent detection of bocavirus DNA in German children with respiratory tract infections

Weißbrich, Benedikt; Neske, Florian; Schubert, Jörg; Tollmann, Franz; Blath, Katharina; Blessing, Kerstin; Kreth, Hans Wolfgang

doi:10.1186/1471-2334-6-109

Cited by 153 publications

(197 citation statements)

References 11 publications

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“…HBoV + cases occurred throughout the year but peaked during late fall and winter (Fig. 2), which is consistent with analogous findings by other authors (Allander et al, 2005;Kesebir et al, 2006;Weissbrich et al, 2006;Chow et al, 2008;Cilla et al, 2008;Martin et al, 2010). The higher frequency of detection during the cold months might partially explain the high rate of co-infection with other respiratory viruses that circulate with a similar pattern, in particular RSV.…”

Section: Resultssupporting

confidence: 80%

“…However, considering the high frequency of HBoV1 detection in infants younger than 1 year and during the fall-winter period, plus the circulation pattern of RSV (Leung et al, 2005), a high rate of HBoV1-RSV co-infection is most likely. Elevated percentages of RSV-HBoV1 co-infections have been observed by others and RSV is commonly cited as the most frequent respiratory virus in co-infection with HBoV1 (Kaplan et al, 2006;Weissbrich et al, 2006;Gerna et al, 2007;Cilla et al, 2008;Pilger et al, 2011). Interestingly, we did not find co-infections in HBoV + samples from adults.…”

Section: Resultscontrasting

confidence: 40%

“…1), indicating a very early incidence of HBoV1 infection. This study and others (Weissbrich et al, 2006;Chow et al, 2008;AlRousan et al, 2011) detected HBoV1 as early as few days after birth; thus HBoV1 infection during the first month of life may not be infrequent. HBoV + cases occurred throughout the year but peaked during late fall and winter (Fig.…”

Section: Resultsmentioning

confidence: 90%

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High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

Ghietto

Cámara

et al. 2012

Journal of Medical Microbiology

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Instituto de Virología 'Dr J. M. Vanella', Facultad de Ciencias Mé dicas, Universidad Nacional de Có rdoba, ArgentinaHuman bocavirus (HBoV) is a parvovirus with a possible aetiological role in respiratory disease that is currently under investigation. We detected HBoV1 in children and adults hospitalized with acute disease of the lower respiratory tract. HBoV genome was detected by PCR in nasopharyngeal swabs collected from 75 patients aged 0-89 years during 2010. HBoV was found in 17/75 (22.7 %) patients, 64.7 % of them infants younger than 1 year old and 29.4 % adults older than 30 years [the bimodal age distribution among HBoV-positive (HBoV + ) patients was statistically significant, P,0.001]. Of all HBoV + cases, 35.3 % were co-infected; all coinfections occurred in children (¡13 years old) and 83.3 % of them were HBoV-respiratory syncytial virus (RSV) co-infections. Among infants younger than 1 year, 50 % HBoV + specimens were co-infected, all of them with RSV. The rate of co-infection in infants was significantly higher compared to the frequency of co-infection in the whole cohort (P50.003). The results suggest that HBoV1 is involved in acute respiratory disease. Interplay between HBoV1 and RSV cannot be discarded as a cause of elevated percentages of co-detections in infants. INTRODUCTIONHuman bocavirus (HBoV) is a parvovirus first identified in 2005 in nasopharyngeal aspirates of children with lower respiratory tract infection (Allander et al., 2005). Since then, it has been associated with upper and lower acute respiratory infection (ARI), a leading cause of acute illnesses worldwide and the most important cause of mortality in infants and young children and disabilityadjusted life-years lost in developing countries (Simoes et al., 2006; WHO, 2009). To date, four species of HBoV have been proposed, and the name human bocavirus 1 (HBoV1) was suggested for the originally discovered virus. HBoV1 is linked to respiratory disease, while it is believed that HBoV2-4 are associated with gastroenteritis (Kapoor et al., 2010). With a ubiquitous distribution, the presence of HBoV DNA has been reported mostly in children with ARI in a variable range from 1.5 to 19 % (Allander, 2008), although recently even higher prevalence (33 %) has been observed in ill children (Martin et al., 2010). Although the virus is associated with ARI, the elevated rates of coinfection with other respiratory viruses with well-established pathogenic potential (Kaplan et al., 2006;Allander et al., 2007;Fry et al., 2007; Gerna et al., 2007;Kleines et al., 2007; Christensen et al., 2008 Christensen et al., , 2010Cilla et al., 2008;Pilger et al., 2011), the detection in asymptomatic individuals (Christensen et al., 2010) and the possibility of a persistent infection (Martin et al., 2010) make it difficult to allocate a causative role for HBoV in respiratory disease. Not only is the aetiological capacity of HBoV under investigation, but the natural history of the infection is still unknown. In addition, the impact of HBoV on the global epid...

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Section: Resultssupporting

confidence: 80%

Section: Resultscontrasting

confidence: 40%

Section: Resultsmentioning

confidence: 90%

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High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

Ghietto

Cámara

et al. 2012

Journal of Medical Microbiology

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“…One possible explanation for the low level of HBoV in surface water could be the low concentration of HBoV excreted in faeces (7.0610 2 to 1.75610 4 copies ml 21 ) (Campe et al, 2008). The incidence of HBoV in German children was estimated to be 10.3 %, with a higher prevalence in winter (Weissbrich et al, 2006). In river water, we detected HBoV throughout the year except in the summer months (Fig.…”

mentioning

confidence: 83%

Detection and quantification of human bocavirus in river water

Hamza

Jurzik

Wilhelm

et al. 2009

Journal of General Virology

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Human bocavirus (HBoV) was recently discovered in children with respiratory-tract infection and has been detected frequently in faecal specimens from children with gastroenteritis. The present study addresses for the first time, to our knowledge, the prevalence of HBoV in river water. By using a newly developed real-time PCR targeting a conserved region of the NP1 gene of HBoV, virus levels in water samples were determined. Moreover, partial sequence analysis of the NP1 gene of HBoV and comparative phylogenetic analysis were performed. HBoV was detected in 40.8 % of collected water samples. The virus level ranged between 3¾10 1 and 2¾10 3 genome equivalents l "1 . Therefore, the present study suggests that river water could play a role in the spread of HBoV. However, further work should be done to determine the actual risk of infection via surface water.

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“…The incidence of HBoV has been reported to be between 1.5% to 11.3% based on tests of respiratory samples from individuals with acute respiratory illness (Allander et al, 2005;Arnold et al, 2006;Bastien et al, 2006;Choi et al, 2006;Foulongne et al, 2006;Lin et al, 2007;Ma et al, 2006;Qu et al, 2007;Sloots et al, 2006;Weissbrich et al, 2006). HBoV appears to be associated with lower respiratory tract infections, and in many cases, co-infection with other respiratory viruses (Allander et al, 2007;Fry et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

Lin

Guan

Cheng

et al. 2008

Journal of Virological Methods

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Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation.

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Frequent detection of bocavirus DNA in German children with respiratory tract infections

Cited by 153 publications

References 11 publications

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

High frequency of human bocavirus 1 DNA in infants and adults with lower acute respiratory infection

Detection and quantification of human bocavirus in river water

ELISAs using human bocavirus VP2 virus-like particles for detection of antibodies against HBoV

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