Detection and quantification of human bocavirus in river water

Hamza, Ibrahim Ahmed; Jurzik, Lars; Wilhelm, Michael; Überla, Klaus

doi:10.1099/vir.0.013557-0

Cited by 42 publications

(35 citation statements)

References 24 publications

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“…As far as we know, only one study has thus far been published on HBoV in river waters, reporting the presence of HBoV in 41% of tested river samples (Hamza et al . ), a similar result to that obtained in the present study (37·5%). Although there is no indication of waterborne HBoV transmission, the widespread presence of this virus in both sewage and surface waters suggests that a potential role of water in its transmission should not be excluded.…”

Section: Resultssupporting

confidence: 93%

The impact of anthropogenic pressure on the virological quality of water from the Tiber River, Italy

Rosa

Sanseverino

Libera

et al. 2017

Lett Appl Microbiol

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To the best of our knowledge, this study constitutes the first attempt to assess the occurrence of enteric viruses in river waters, in areas differentially influenced by anthropogenic pressure. Enteric viruses (enterovirus, norovirus, adenovirus, rotavirus, hepatitis A and E viruses, and bocavirus) were widespread in the industrial and urban areas, and were less frequently detected in the agricultural area. Interestingly, human bocavirus was the most frequently detected virus, outnumbering even adenoviruses, known to be widespread in water environments. The widespread presence of bocavirus in surface waters suggests that a potential role of water in its transmission should not be excluded.

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Section: Resultssupporting

confidence: 93%

The impact of anthropogenic pressure on the virological quality of water from the Tiber River, Italy

Rosa

Sanseverino

Libera

et al. 2017

Lett Appl Microbiol

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“…Indeed, Hamza and collaborators described the detection of HBoV in 41 % of river water samples (Hamza et al 2009). Also, Li and coworkers recently detected HBoV along with other enteric viruses in sewage-contaminated fresh and marine waters (Li et al 2016).…”

Section: Discussionmentioning

confidence: 96%

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

et al. 2016

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The prevalence and genetic diversity of human bocaviruses (HBoVs) in sewage water samples are largely unknown. In this study, 134 raw sewage samples from 25 wastewater treatment plants (WTPs) in Italy were analyzed by nested PCR and sequencing using species-specific primer pairs and broad-range primer pairs targeting the capsid proteins VP1/VP2. A large number of samples (106, 79.1 %) were positive for HBoV. Out of these, 49 were classified as HBoV species 2, and 27 as species 3. For the remaining 30 samples, sequencing results showed mixed electropherograms. By cloning PCR amplicons and sequencing, we confirmed the copresence of species 2 and 3 in 29 samples and species 2 and 4 in only one sample. A real-time PCR assay was also performed, using a newly designed TaqMan assay, for quantification of HBoVs in sewage water samples. Viral load quantification ranged from 5.51E+03 to 1.84E+05 GC/L (mean value 4.70E+04 GC/L) for bocavirus 2 and from 1.89E+03 to 1.02E+05 GC/L (mean value 2.27E+04 GC/L) for bocavirus 3. The wide distribution of HBoV in sewages suggests that this virus is common in the population, and the most prevalent are the species 2 and 3. HBoV-4 was also found, representing the first detection of this species in Italy. Although there is no indication of waterborne transmission for HBoV, the significant presence in sewage waters suggests that HBoV may spread to other water environments, and therefore, a potential role of water in the HBoV transmission should not be neglected.

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“…allergen‐specific sensitization) was not associated with HBoV1 infection among wheezing children . Finally, contact with sewage or river water may also increase HBoV infection frequency .…”

Section: Risk Factorsmentioning

confidence: 88%

Human bocavirus—the first 5 years

Jartti

Hedman

Jartti

et al. 2011

Reviews in Medical Virology

245

278

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Four species of human bocavirus (HBoV) have been recently discovered and classified in the Bocavirus genus (family Parvoviridae, subfamily Parvovirinae). Although detected both in respiratory and stool samples worldwide, HBoV1 is predominantly a respiratory pathogen, whereas HBoV2, HBoV3, and HBoV4 have been found mainly in stool. A variety of signs and symptoms have been described in patients with HBoV infection including rhinitis, pharyngitis, cough, dyspnea, wheezing, pneumonia, acute otitis media, fever, nausea, vomiting, and diarrhea. Many of these potential manifestations have not been systematically explored, and they have been questioned because of high HBoV co-infection rates in symptomatic subjects and high HBoV detection rates in asymptomatic subjects. However, evidence is mounting to show that HBoV1 is an important cause of lower respiratory tract illness. The best currently available diagnostic approaches are quantitative PCR and serology. This concise review summarizes the current clinical knowledge on HBoV species.

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Detection and quantification of human bocavirus in river water

Cited by 42 publications

References 24 publications

The impact of anthropogenic pressure on the virological quality of water from the Tiber River, Italy

The impact of anthropogenic pressure on the virological quality of water from the Tiber River, Italy

Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

Human bocavirus—the first 5 years

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