Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus

Park, Yu Shin; Song, Ok-Kyu; Hong, Suk Whan; Kwak, June M.; Cho, Moo Je; Nam, Hong Gil

doi:10.1007/bf00020237

Cited by 7 publications

(14 citation statements)

References 20 publications

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“…They may result from DNA mispairing between two adjacent, short, directrepeat sequences (Tautz et al, 1986;Levinson and Gutman, 1987). Similar sequence length variations have also been observed in protein kinases (Park et al, 1995) and prolinerich cell wall proteins (Schmidt et al, 1994).…”

Section: Discussionsupporting

confidence: 70%

Characterization of oleosins in the pollen coat of Brassica oleracea.

et al. 1997

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Mature Brassica oleracea pollen grains are covered with a lipophilic pollen coat containing a variety of proteins. Screening of an anther cDNA expression library for the coding sequences of such proteins resulted in the isolation of a number of cDNA clones encoding glycine-rich oleosins. The proteins were shown to be attached to the lipophilic coat material only and to be absent elsewhere in the plant. Within the coat, severa1 forms of the pollen coat oleosin with different molecular weíghts were detected. The forms are encoded by different transcripts that originate from a single gene. Expression of this gene is restricted to the tapetum and is quantitatively regulated by the water content of the anther. Similar oleosins were found in the pollen coat of 6. alboglobra and 6. napus.

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Section: Discussionsupporting

confidence: 70%

Characterization of oleosins in the pollen coat of Brassica oleracea.

et al. 1997

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“…An amino acid sequence comparison between SPK-3 and SPK-4 reveals 93% overall sequence identity and 95% identity within the catalytic domain. Homology searches in databases (GenBank, EMBL and PIR) using the catalytic domain of SPK-3 (from Met 1 to Phe 260 ) showed that the two SPKs are closely related to the Arabidopsis ASK2 protein kinase (91% identity; Park et al 1993) and an ABA-inducible protein kinase, PKA-BA1 (82% identity; Anderberg and Walker-Simmons, 1992), from wheat. Although the degree of identity was relatively low, at 30±40%, [SPK-3 and SPK-4 also showed] sequence similarity to yeast SNF1 (Celenza and Carlson, 1986), barley cBKIN12 (Halford et al 1992), yeast nim1 + (Russell and Nurse, 1987), yeast Ca 2+ / calmodulin-dependent protein kinase (Ohya et al 1991), and soybean CDPK (Harper et al 1991).…”

Section: Resultsmentioning

confidence: 99%

“…SPK-3 and SPK-4 contain highly acidic regions at their C-terminal ends which are thought to represent the regulatory domains of these kinases (Park et al 1993). The acidic regions encoded by the SPK-3 and SPK-4 cDNA clones contain stretches of 18 and 16 Glu/Asp residues (amino acid positions, 317 to 334 and 317 to 332), respectively.…”

Section: Resultsmentioning

confidence: 99%

Differential expression of two functional serine/threonine protein kinases from soyabean that have an unusual acidic domain at the carboxy terminus

Yoon¹,

Kim²,

Shin³

et al. 1997

Mol Gen Genet

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Two soybean cDNA clones, SPK-3 and SPK-4, encoding putative protein kinases were isolated and characterized. Both cDNAs encoded approximately 40-kDa serine/threonine kinases with unusual stretches of acidic amino acids in their carboxy-terminal regions, which are highly homologous to PKABA1 from wheat and ASKs from Arabidopsis. These kinases are encoded by one- or two-copy genes in the soybean genome. Notably, SPK-3 and -4 showed different patterns of expression in various soybean tissues. SPK-3 is highly expressed in dividing and elongating tissues of young seedlings but relatively weakly in tissues of mature plants. In contrast, SPK-4 showed relatively high and constitutive expression in all the tissues examined except for leaf tissues of mature plants. Although various stressors, such as dehydration and high salinity, increased the expression of both genes, the induction kinetics were different. The two genes also differed in their response to abscisic acid (ABA). SPK-3 was induced but SPK-4 was not affected by exogenously supplied abscisic acid. In accordance with these expression data analysis of the activity of a chimeric SPK-3 promoter::beta-glucuronidase (GUS) reporter gene by transient expression in tobacco leaves confirmed the inducibility of SPK-3 by salt and ABA. Polyclonal antibodies raised against a recombinant SPK-4 protein produced in Escherichia coli specifically recognized both recombinant SPK-3 and -4 proteins. Kinase assays using affinity-purified SPK-4/ antibody complexes with crude soybean extracts as substrate identified specific phosphorylation of two 41 and 170 kDa soybean proteins that were phosphorylated on serine residues. Taken together, our results suggest that SPK-3, and/or SPK-4 are functional serine protein kinase(s). Furthermore, SPK-3 and -4 may play different roles in the transduction of various environmental stresses.

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“…The B. napus C genome has been identified and mapped in a number of Brassica populations by several groups and has been determined to comprise nine linkage groups: N11-N19 (Slocum et al 1990;Park et al 1995;Sharpe et al 1995;Piquemal et al 2005;Sun et al 2007). Using the approaches described above, we identified the nine C-genome chromosomes of B. carinata.…”

Section: Generation Of B-genome Interspecific Linesmentioning

confidence: 99%

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

et al. 2011

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Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the B. carinata genome and to study the genetic changes that occur during the introgression process. We mapped the B and C genomes of B. carinata with SSR markers and observed their introgression into B. napus through a number of backcross generations, focusing on a BC 3 and BC 3 S 1 sibling family. There was close colinearity between the C genomes of B. carinata and B. napus and we provide evidence that B. carinata C chromosomes pair and recombine normally with those of B. napus, suggesting that similar to other Brassica allotetraploids no major chromosomal rearrangements have taken place since the formation of B. carinata. There was no evidence of introgression of the B chromosomes into the A or C chromosomes of B. napus ; instead they were inherited as whole linkage groups with the occasional loss of terminal segments and several of the B-genome chromosomes were retained across generations. Several BC 3 S 1 families were analyzed using SSR markers, genomic in situ hybridization (GISH) assays, and chromosome counts to study the inheritance of the B-genome chromosome(s) and their association with morphological traits. Our work provides an analysis of the behavior of chromosomes in an interspecific cross and reinforces the challenges of introgressing novel traits into crop plants.

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Frequent in-frame length variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus

Cited by 7 publications

References 20 publications

Characterization of oleosins in the pollen coat of Brassica oleracea.

Characterization of oleosins in the pollen coat of Brassica oleracea.

Differential expression of two functional serine/threonine protein kinases from soyabean that have an unusual acidic domain at the carboxy terminus

Analysis of B-Genome Chromosome Introgression in Interspecific Hybrids of Brassica napus × B. carinata

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