Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Hou, Yansong; Liang, Wei; Zhang, Liping; Cheng, Shuiping; He, Feng; Wu, Zhenbin

doi:10.1007/s11783-010-0283-1

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…Complete structure evaluation was performed by many researchers to study bioactive natural products, and methods used were: 1D, 2D NMR, MS/MS, HPLC and chiral GC-MS analysis [163][164][165][166].…”

Section: Capillary Electrophoresis Using Diode Array Detection (Ce-dad)mentioning

confidence: 99%

Methods for Upstream Extraction and Chemical Characterization of Secondary Metabolites from Algae Biomass

Alassali¹,

Cybulska²

2015

Adv Tech Biol Med

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Marine life is very rich in producing various and distinctive chemical components, both basic and complex. Due to the harsh conditions such as high salinity, deficiency of nutrients, light and space, which make the marine environment competitive, organisms adapt to the environment by producing various chemicals and metabolites to help them survive under such conditions. In many studies great emphasis has been given to the secondary metabolites produced by algae (macro and microalgae). Certain species of algae are known for their high content of fatty acids, fibers, antioxidants, carotenoids, sterols, proteins, phytocolloids, lectins, oils, amino acids, unsaturated fatty acids, and vitamins, which could be commercially utilized. Current algae studies emphasize on four main research areas: fuels, bioactive metabolites, toxins, and chemical ecology. This paper focuses on reviewing interesting biochemicals from algae biomass and their therapeutic applications. To achieve optimum extraction of high-value products, extraction methods and conditions were thoroughly presented in this review. Finally, different analytical approaches and techniques to identify the extracted chemicals were discussed.

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Section: Capillary Electrophoresis Using Diode Array Detection (Ce-dad)mentioning

confidence: 99%

Methods for Upstream Extraction and Chemical Characterization of Secondary Metabolites from Algae Biomass

Alassali¹,

Cybulska²

2015

Adv Tech Biol Med

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“…The use of this technique in freshwater is not so usual as it is marine or estuarine waters, (Descy et al., 2009;Simmons et al, 2016), but it has shown effectiveness monitoring the phytoplankton community in lakes (Fietz & Nicklisch, 2004;Descy et al, 2005;Picazo et al, 2013). HPLC allows rapid and automated analysis of lipophilic photosynthetic pigments (Jeffrey et al, 1999) with a high differentiating power of the higher taxonomic categories (Hou et al, 2011). On the other hand, microscopic techniques are able to discriminate at the level of genus or species, but present difficulties in the identification of nanoplankton and picoplankton, commonly well represented in oligotrophic systems, and also the reproducibility is lower than with chromatographic techniques (Goericke & Montoya, 1998;Fietz & Nicklisch, 2004).…”

Section: Introductionmentioning

confidence: 99%

Changes in the phytoplankton community structure in a monomictic temperate lake

Llorente¹,

Seoane²

2020

Limnetica

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Changes in the phytoplankton community structure in a monomictic temperate lakeThis study focused on the phytoplanktonic community of lake Sanabria (NW Spain) during the mixing period. We integrated the classic phytoplankton counting method and the pigment analysis via high performance liquid chromatography, to obtain a global image of the community structure, which was very similar among the study zones and unaltered despite a temporal increasing of biomass during this period. The diatom Asterionella formosa Hassall (around 80 %), and consequently the fucoxanthin were the species and the secondary pigment more abundant, respectively. This dominance contrasted with the studies performed in 90´s, where the community structure was distributed among chlorophytes, cryptophytes, diatoms, dinoflagellates and cyanophytes, with no clear and persistent dominance of any of the main taxonomic groups and an occasional presence of Asterionella formosa. However, the classical variables, such as nutrients or chlorophyll a concentration, used for trophic definition have barely changed since the studies of 90's. It is possible that other parameters, such as the residence time or the effect of the increase in the temperature since 90´s could have influenced the phytoplankton community structural changes observed.

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“…However, chemotaxonomy studies on other plants showed that chemical compounds (e.g., Sesquiterpene dialdehyde, etc) could be considered as species markers [22]. Furthermore, Hou et al [23] found that chemotaxonomic classification could be very useful for aquatic assessment in distinguishing phytoplankton communities and extremely advantageous and cost-effective in large ecosystem-scale research. Li et al [24] found that the evolution and classification of bamboos inferred from leaf wax n-alkanes were consistent with morphological investigations reported previously.…”

Section: Introductionmentioning

confidence: 99%

Chemotaxonomic Study of Citrus, Poncirus and Fortunella Genotypes Based on Peel Oil Volatile Compounds - Deciphering the Genetic Origin of Mangshanyegan (Citrus nobilis Lauriro)

et al. 2013

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Volatile profiles yielded from gas chromatography-mass spectrometry (GC-MS) analysis provide abundant information not only for metabolism-related research, but also for chemotaxonomy. To study the chemotaxonomy of Mangshanyegan, its volatile profiles of fruit and leaf and those of 29 other genotypes of Citrus, Poncirus, and Fortunella were subjected to phylogenetic analyses. Results showed that 145 identified (including 64 tentatively identified) and 15 unidentified volatile compounds were detected from their peel oils. The phylogenetic analysis of peel oils based on hierarchical cluster analysis (HCA) demonstrated a good agreement with the Swingle taxonomy system, in which the three genera of Citrus, Poncirus, and Fortunella were almost completely separated. As to Citrus, HCA indicated that Citrophorum, Cephalocitrus, and Sinocitrus fell into three subgroups, respectively. Also, it revealed that Mangshanyegan contain volatile compounds similar to those from pummelo, though it is genetically believed to be a mandarin. These results were further supported by the principal component analysis of the peel oils and the HCA results of volatile profiles of leaves in the study.

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Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

Cited by 9 publications

References 15 publications

Methods for Upstream Extraction and Chemical Characterization of Secondary Metabolites from Algae Biomass

Methods for Upstream Extraction and Chemical Characterization of Secondary Metabolites from Algae Biomass

Changes in the phytoplankton community structure in a monomictic temperate lake

Chemotaxonomic Study of Citrus, Poncirus and Fortunella Genotypes Based on Peel Oil Volatile Compounds - Deciphering the Genetic Origin of Mangshanyegan (Citrus nobilis Lauriro)

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