FRET based ‘red-switch’ for Al³⁺ over ESIPT based ‘green-switch’ for Zn²⁺: dual channel detection with live-cell imaging on a dyad platform

Abstract

Our designed chemosensor, rhodamine-HBT-dyad (RHD), selectively detects two biologically important ions (Al3+ and Zn2+) at two different wavelengths (red and green, respectively) through FRET and ESIPT in vitro and in vivo.

Recognition of Mg2+ and Zn2+ based on a naphthalene-based fluorescent probe by regulating solvents

“…The above experiments suggested the rhodamine core always was in the ring closed isomeric form both in the presence of Mg 2+ and Zn 2+ and some reported fluorescent probes also shared some similarities to HL [37][38][39][40][41][42][43], the selective recognition of HL for Mg 2+ /Zn 2+ should be attributed to the interaction of the naphthalene moieties with Zn 2+ /Mg 2+ which inhibited photo-induced electron transfer (PET) process rather than the ring-opening of the rhodamine spirolactam. As shown in Scheme 2, it seemed that the lone pair electrons from the nitrogen atom of the AC@N group to naphthalene moieties was responsible for the photoinduced electron-transfer (PET) process, which quenched fluorescence emission of the sensor.…”

Development of a selective fluorescent ligand for aluminum ion having a 6-chloro-pyridazine moiety

“…In addition, we believe that excited-state intramolecular proton transfer (ESIPT) and photo-induced electron transfer (PET) [5,21,22] might be involved in the emission process of the CPCH-Al(III) complex, because the same chemical moiety, Fig. S3), which was previously reported to be a Al(III)-chemosensor that exhibited ESIPT and PET [22], is present in the chemical structure of CPCH (Fig.…”

A FRET-based Ratiometric Fluorescent Probe for Nitroxyl Detection in Living Cells

“…[7][8][9][21][22][23][24][25] The energy of the excited donor fluorophore can be transferred to the acceptor through non-radiative dipole-dipole coupling. [26][27][28][29][30][31][32][33] To our best knowledge, no FRET-based ratiometric probes for detecting HNO has been reported to date. Herein we have designed and synthesized the first FRET-based ratiometric probe for HNO and applied the probe for living cell imaging studies.…”