Frist Detection of The blaOXA-23 Genes in Clinical Isolates of Acinetobacterbaumanniiin Babylon Hospitals-Iraq

AL-Harmoosh, Raad A.; Jarallah, Eman M.; AL-Shamari, Anmar M.; Al-Khafaji, Hussein M. H.; Jabuk, Sura I.A.; AL-Khaweledy, Essam M.

doi:10.9790/3008-1104024448

Cited by 4 publications

(7 citation statements)

References 21 publications

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“…The results indicated that 5% of isolates were categorized as MDR, while the remaining 95% were classified as XDR. These findings contradict the reports of [13,22,23,30] which reported higher MDR rates and relatively lower XDR rate.…”

Section: Discussioncontrasting

confidence: 99%

“…Notably, all isolates were completely resistant to ampicillin, while a significant proportion (96.6%) exhibited resistance to pipracillin. These resistance patterns are consistent with previous studies [21][22][23]reporting similar findings for ampicillin. However, there were discrepancies in the resistance rates for pipracillin compared to studies conducted by [21,[24][25][26].…”

Section: Discussionsupporting

confidence: 93%

“…These resistance patterns are consistent with previous studies [21][22][23]reporting similar findings for ampicillin. However, there were discrepancies in the resistance rates for pipracillin compared to studies conducted by [21,[24][25][26]. Resistance rates for beta-lactam combinations varied as well.…”

Section: Discussionsupporting

confidence: 93%

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Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Jasim,

Hadi,

Alsherees

et al. 2023

Med.Sci.Jour.Adv.Res

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Acinetobacter baumannii is a highly problematic bacterium due to its multidrug-resistant nature and ability to rapidly develop resistance. Carbapenem-resistant A. baumannii (CRAB) is particularly concerning, recognized as a top priority pathogen by the World Health Organization (WHO). In this study, we aimed to assess the susceptibility of A. baumannii to antibiotics and characterize the presence of specific carbapenem resistance genes using PCR analysis. The study was conducted as a cross-sectional investigation at Al-Sader Medical City and Baghdad Teaching Hospitals between October 2022 and February 2023. A total of 59 A.baumannii isolates were collected from patients. To ensure accuracy in diagnosis, the isolates underwent thorough processing using morphological techniques, biochemical tests, and Vitek2 systems. The susceptibility of the isolates to 24 antibiotics was evaluated using the Kirby-Bauer method. Additionally, DNA extraction and PCR analysis were performed to detect the presence of carbapenem resistance genes. The study included analysis of 59 specimens collected from patients, including sputum, wound swabs, blood, and inguinal swabs. The majority of the isolates were obtained from in-patients, demonstrating a significant difference compared to outpatients. Among the studied isolates, the most prevalent carbapenem resistance gene detected was blaOXA51, present in 97% of the isolates. This highlights the substantial presence of blaOXA-mediated carbapenem resistance among A. baumannii strains in our study population. Furthermore, our findings revealed alarmingly high resistance rates against the majority of antibiotics commonly used to treat A. baumannii infections. Approximately 95% of the isolates were classified as extensively drug-resistant, indicating resistance to multiple classes of antibiotics. This poses significant challenges in terms of effective treatment options and emphasizes the urgent need for alternative strategies to manage A. baumannii infections.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 93%

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Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Jasim,

Hadi,

Alsherees

et al. 2023

Med.Sci.Jour.Adv.Res

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“…It is of interest that SIM-1 was produced by one strain of MDR K. pneumoniae intermediately susceptible to aztreonam and colistin. Similar results were reported for the SIM-1 gene in A. baumannii 10 in Iraq 22 and in Egypt, 20 in P. aeruginosa and recently in Egypt 23 and K. pneumoniae with ST23 strain carrying SIM-1 in China. 24 The GIM-1 and SIM-1 genes were transferred to Sudan, these can be broadly categorized into issues of emergence, spread, and clonal expansion with diversity, which include natural genetic diversification caused by mutation, horizontal gene transfer among other species, an increase in international travel and patient transfer between countries raising the possibility for further dissemination of strains carrying resistant genes.…”

Section: Discussionsupporting

confidence: 87%

The Emergence of Carbapenem Resistant Enterobacteriaceae Producing GIM-1 and SIM-1 Clinical Isolates in Khartoum-Sudan

Bayoumi¹,

Hamid²

2022

IDR

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Purpose The aim of this study was to detect multidrug resistant GIM-1 and SIM-1 producing Enterobacteriaceae clinical isolates from hospitalized patients across three Khartoum State Teaching Hospitals, Sudan. Patients and Methods From May 2018 to October 2019, Enterobacteriaceae clinical isolates from inpatients admitted to different Khartoum state hospitals. Genes for carbapenemase (GIM-1 and SIM-1) were amplified by polymerase chain reaction (PCR). Agar dilution method was used to determine MICs for imipenem and meropenem after antimicrobial susceptibility testing. Results Five (1.29%) isolates of Enterobacteriaceae [2 (0.51%) Escherichia coli isolates produce GIM-1, 2 (0.51%) Klebsiella pneumoniae isolates (one [0.25%] of each produce of GIM-1 and of SIM-1), and 1 (0.25%) Enterobacter cloacae isolate produce GIM-1]. Susceptibility profiling of the isolates showed a low-level resistance to imipenem and meropenem MICs (8, 16 and 32 μg/mL). It also had resistance to ampicillin, extended-spectrum cephalosporin’s, aztreonam, and amoxicillin-clavulanate and with the two K. pneumoniae strains showing resistance to colistin. Conclusion We report the emergence of four GIM-1 producing Enterobacteriaceae strains and one strain of SIM-1 producing K. pneumoniae genes, isolated from hospitalized patients, with a high resistance pattern to antimicrobial agents. Whole-genome sequencing (WGS) is necessary for precise identification of clonal diversity backgrounds of acquired carbapenemase genes in diagnostic laboratories as the number of cases of carbapenem resistant Enterobacteriaceae infection increases annually.

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“…Top exhibit resistance most antimicrobials that counseled by CLSI eight and gave the impression to be intensive antibiotic resistance (XDR) this might make a severe problem for gold choice of therapy (Mikkili et al, 2015) this result was more identi ied with the report of emergence XDR during A . baumannii isolates from patients in ICUs of Samsung medical center in national capital, South Korea (Flonta et al, 2011) Hence, The incidence of isolates contain blaIMP in Babylon hospitals which explained our many factors contributed in spread resistance by transfer plasmid or integron additional to present IS, resistant of many isolates might produce identical restriction pattern recommend that result in same line with previous study (Chang et al, 2013) (Al-Harmoosh et al, 2016The presence of class one integrons and IS elements are always con-nected to the epidemic potential of A. baumannii our result come closed with previous study (Turton et al, 2005) (Bratu et al, 2008).…”

Section: Resultssupporting

confidence: 71%

Molecular analysis of multi –drug resistant acintobacter baumannii isolated in baghdad hospital

Ridha¹,

Ali²,

Jassim³

2019

ijrps

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Qurum sense is regulatory systems that enable bacteria to expretion for a specific set of genes by making collective decisions and regulation of virulence factors of pathogenic Acintobacter baumannii, the prevalence of carbapenem resistance among (8) different antibiotic groups pattern. In the MDR index, all isolates showed high MDR, ranged from (6-21) antibiotics. MDR index is between (0.29-1.0).Quantitative biofilm production assay showed that 118/124(95.1%)of isolates were biofilm-formers. Detection of QS genes showed that (97.6%)121/124,(98.3%)122\124 and (4.8%)of isolates carried luxR, rhlI and rhIR genes, respectivelly. All isolates possess rhIR gene are isolated from child meningococcal patients and characterized by the strong biofilm formation. Identification of abaI gene in A.baumannii producing the QS signal molecules detected in124\124(100%). The current study revealed a strong relationship between Q.S genes luxR,rhII,rhIR and oxacillinases presence OXA23, OXA69. While a strong negative correlation between OXA48 and OXA69 with the rhIR gene. All isolates carried Integron I(n=124)(100%) with a significant presence of (luxR, rhII) genes, while INT2,3 had a highly significant level with rhIR. Noteworthy, (n=105) 84.7% of OXA51 positive isolates have carried ISAbal-blaOXA-51, whereas all isolates which have OXA23 carried ISAbal-blaOXA-23(n=65)52.4% most of them revealed IMP&MEM resistance phenotype. Insertion sequence(ISAbal-blaOXA-51) showed significant correlation with luxR, rhII genes, while there was no correlation among ISAbal25, ISAbal, ISAbal25-blaADC and luxR, rhII. Positive correlation recorded with all IS with rhIR, except ISAbal-blaADC.ads-a gene was observed in 77/124(62.1%) of isolates, which associated with aminoglycoside resistance, and included within the 93 XDR isolates. Studied isolates showed the same prevelance rate for ompA, smpA which was122\124(98.4%) of isolates.

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Frist Detection of The blaOXA-23 Genes in Clinical Isolates of Acinetobacterbaumanniiin Babylon Hospitals-Iraq

Cited by 4 publications

References 21 publications

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

Carbapenem Resistance Mechanisms in Acinetobacter baumannii: Spotlight on OXA Genes

The Emergence of Carbapenem Resistant Enterobacteriaceae Producing GIM-1 and SIM-1 Clinical Isolates in Khartoum-Sudan

Molecular analysis of multi –drug resistant acintobacter baumannii isolated in baghdad hospital

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