1996
DOI: 10.1021/ac9601775
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Fritless Packed Columns for Capillary Electrochromatography:  Separation of Uncharged Compounds on Hydrophobic Hydrogels

Abstract: A novel column is described that does not require frits to keep packing material within a capillary. A continuous bed is prepared in situ in aqueous solution by radical copolymerization of N-isopropylacrylamide and 2-acrylamido-2-methylpropanesulfonic acid (the resultant gel is denoted poly(AMPS-co-IPAAm). N,N'-Methylenebisacrylamide is used for cross-linking. On the application of an electrical field, electroosmotic flow (EOF) is developed in the bed along the capillary, where fluid propulsion would be otherw… Show more

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Cited by 149 publications
(65 citation statements)
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“…Slightly crosslinked gels and liquid (non-crosslinked) gels were employed for the enantiomer separations of Dns-AAs, homatropine and atropine. Koide and Ueno 29,30 prepared charged gel columns according to the previously reported method [31][32][33] with some modifications. A mixture of either poly β-CD or caboxymethyl β-CD polymers (chiral selector), Tween 20, AAm, BIS and AMPS (for the preparation of negatively charged gels) or N-(2-acrylamidoethyl)triethylammonium iodide (for the preparation of positively charged gels) in a Tirs-boric acid buffer (pH 8.1) containing ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED) was introduced into a fused silica capillary and allowed to stand for >5 h. The resultant gels generated substantial EOF, the direction of which depended on the charged monomer added, and therefore could be used to separate neutral enantiomers.…”
Section: Homogeneous Gel Columnsmentioning
confidence: 99%
“…Slightly crosslinked gels and liquid (non-crosslinked) gels were employed for the enantiomer separations of Dns-AAs, homatropine and atropine. Koide and Ueno 29,30 prepared charged gel columns according to the previously reported method [31][32][33] with some modifications. A mixture of either poly β-CD or caboxymethyl β-CD polymers (chiral selector), Tween 20, AAm, BIS and AMPS (for the preparation of negatively charged gels) or N-(2-acrylamidoethyl)triethylammonium iodide (for the preparation of positively charged gels) in a Tirs-boric acid buffer (pH 8.1) containing ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED) was introduced into a fused silica capillary and allowed to stand for >5 h. The resultant gels generated substantial EOF, the direction of which depended on the charged monomer added, and therefore could be used to separate neutral enantiomers.…”
Section: Homogeneous Gel Columnsmentioning
confidence: 99%
“…Over the last 20 years CE has demonstrated to be a fast and versatile analytical technique that combines simplicity with high reproducibility [2]. Narrow-diameter polyimide-coated fused silica capillaries assures flexibility and allows efficient heat dissipation, thereby permits the use of high field strength resulting in separation efficiencies over [105][106] where Ld is the length of the capillary to the detector, & is the migration time, and V is the applied voltage. where eo is the dielectric constant, y is the viscosity of the buffer, and 5 is the zeta potential on the surface.…”
Section: Capillary Electrophoresismentioning
confidence: 99%
“…At present, capillaries packed with the typical stationary phase for HPLC are used in various CEC studies. 3,4 On the other hand, monolithic stationary phases, [5][6][7][8][9][10][11][12][13][14][15] which are ungranular polymeric separation media, are attracting increasing attention in the field of CEC due to their simple preparation methods, wide variety of functionalization and good stability.…”
mentioning
confidence: 99%