From adenoma to CRC stages: the oral-gut microbiome axis as a source of potential microbial and metabolic biomarkers of malignancy

Russo, Edda; Gloria, Leandro Di; Nannini, Giulia; Niccolai, Elena; Ringressi, Maria Novella; Baldi, Simone; Fani, Renato; Tenori, Leonardo; Taddei, Antonio; Ramazzotti, Matteo; Amedei, Amedeo

doi:10.1016/j.neo.2023.100901

Cited by 16 publications

(4 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2f ). We further tested this observation on publicly available 16S rRNA gene sequencing data from paired tumour tissue and saliva samples from patients with CRC 47 . To gain resolution to the Fna clade level, we identified Fna clade-specific amplicon sequence variants 48 ( Methods and Supplementary Table 8 ).…”

Section: An Fna Clade Enriched In the Crc Nichementioning

confidence: 99%

See 1 more Smart Citation

A distinct Fusobacterium nucleatum clade dominates the colorectal cancer niche

Zepeda-Rivera,

Minot,

Bouzek

et al. 2024

Nature

View full text Add to dashboard Cite

Fusobacterium nucleatum (Fn), a bacterium present in the human oral cavity and rarely found in the lower gastrointestinal tract of healthy individuals1, is enriched in human colorectal cancer (CRC) tumours2–5. High intratumoural Fn loads are associated with recurrence, metastases and poorer patient prognosis5–8. Here, to delineate Fn genetic factors facilitating tumour colonization, we generated closed genomes for 135 Fn strains; 80 oral strains from individuals without cancer and 55 unique cancer strains cultured from tumours from 51 patients with CRC. Pangenomic analyses identified 483 CRC-enriched genetic factors. Tumour-isolated strains predominantly belong to Fn subspecies animalis (Fna). However, genomic analyses reveal that Fna, considered a single subspecies, is instead composed of two distinct clades (Fna C1 and Fna C2). Of these, only Fna C2 dominates the CRC tumour niche. Inter-Fna analyses identified 195 Fna C2-associated genetic factors consistent with increased metabolic potential and colonization of the gastrointestinal tract. In support of this, Fna C2-treated mice had an increased number of intestinal adenomas and altered metabolites. Microbiome analysis of human tumour tissue from 116 patients with CRC demonstrated Fna C2 enrichment. Comparison of 62 paired specimens showed that only Fna C2 is tumour enriched compared to normal adjacent tissue. This was further supported by metagenomic analysis of stool samples from 627 patients with CRC and 619 healthy individuals. Collectively, our results identify the Fna clade bifurcation, show that specifically Fna C2 drives the reported Fn enrichment in human CRC and reveal the genetic underpinnings of pathoadaptation of Fna C2 to the CRC niche.

show abstract

Section: An Fna Clade Enriched In the Crc Nichementioning

confidence: 99%

“…NS, not significant. g , Graph shows the percent relative abundance of Fna C1 (green) and Fna C2 (lavender) in paired saliva (circle) or tumor biopsy (triangle) samples from 39 patients with colorectal adenocarcinomas 47 . Data is plotted as mean ± s.e.m.…”

Section: Extended Data Figures and Tablesmentioning

confidence: 99%

A distinct Fusobacterium nucleatum clade dominates the colorectal cancer niche

Zepeda-Rivera,

Minot,

Bouzek

et al. 2024

Nature

View full text Add to dashboard Cite

show abstract

“…GI cancers and also their treatments, such as chemotherapy, have been consistently demonstrated to induce changes in the GM composition and functions [56], contributing to GI-M severity and to malnutrition onset [57,58]. In this context, dietary interventions aimed at fostering a healthy GM before therapy and enhancing its resilience during and after therapy show potential as therapeutic strategies for GI-M and its related symptoms [59,60].…”

Section: Immunonutrition and Gut Microbiota Modulationmentioning

confidence: 99%

Synergistic Strategies for Gastrointestinal Cancer Care: Unveiling the Benefits of Immunonutrition and Microbiota Modulation

Martinelli,

Lamminpää,

Dübüş

et al. 2023

Nutrients

Self Cite

View full text Add to dashboard Cite

Gastrointestinal (GI) cancers are a group of highly prevalent malignant tumors affecting the gastrointestinal tract. Globally, one in four cancer cases and one in three cancer deaths are estimated to be GI cancers. They can alter digestive and absorption functions, leading to severe malnutrition which may worsen the prognosis of the patients. Therefore, nutritional intervention and monitoring play a fundamental role in managing metabolic alterations and cancer symptoms, as well as minimizing side effects and increasing the effectiveness of chemotherapy. In this scenario, the use of immunonutrients that are able to modulate the immune system and the modification/regulation of the gut microbiota composition have gained attention as a possible strategy to improve the conditions of these patients. The complex interaction between nutrients and microbiota might contribute to maintaining the homeostasis of each individual’s immune system; therefore, concurrent use of specific nutrients in combination with traditional cancer treatments may synergistically improve the overall care of GI cancer patients. This work aims to review and discuss the role of immunonutrition and microbiota modulation in improving nutritional status, postoperative recovery, and response to therapies in patients with GI cancer.

show abstract

“…It may also be caused by lifestyle, food, nutritional intake, environmental circumstances, and the microbiome. These variables affect non-neoplastic cells, including immune cells, increasing heterogeneity [6,7]. CRC is caused mainly by immune system malfunction, as it begins by impairing the host's anti-tumor immunity, the so-called tumor microenvironment [8].…”

Section: Introductionmentioning

confidence: 99%

Interaction between Gut Microbiota and Dendritic Cells in Colorectal Cancer

Zaher,

Basingab

2023

Biomedicines

View full text Add to dashboard Cite

Colorectal cancer (CRC) is a malignancy that manifests in serial stages and has been observed to have an escalating incidence in modern societies, causing a significant global health problem. The development of CRC is influenced by various exogenous factors, including lifestyle, diet, nutrition, environment, and microbiota, that can affect host cells, including immune cells. Various immune dysfunctions have been recognized in patients with CRC at different stages of this disease. The signature of microbiota in the development of CRC—inflammation related to obesity, diet, and reactive host cells, such as dendritic cells (DCs)—has been highlighted by many studies. This study focuses on DCs, the primary cellular mediators linking innate and adaptive immune responses against cancer. In addition, this review focuses on the role of microbiota in dysbiosis and how it affects DCs and, in turn, the immune response and progression of CRC by stimulating different sets of T cells. Additionally, DCs’ role in protecting this delicate balance is examined. This is to determine how gene yields of commensal microbiota may be critical in restoring this balance when disrupted. The stages of the disease and major checkpoints are discussed, as well as the role of the C-type lectin receptor of immature DCs pattern recognition receptor in CRC. Finally, based on a thorough examination of worldwide clinical studies and recent advancements in cancer immunotherapy, it is recommended that innovative approaches that integrate DC vaccination strategies with checkpoint inhibitors be considered. This approach holds great promise for improving CRC management.

show abstract

From adenoma to CRC stages: the oral-gut microbiome axis as a source of potential microbial and metabolic biomarkers of malignancy

Cited by 16 publications

References 66 publications

A distinct Fusobacterium nucleatum clade dominates the colorectal cancer niche

A distinct Fusobacterium nucleatum clade dominates the colorectal cancer niche

Synergistic Strategies for Gastrointestinal Cancer Care: Unveiling the Benefits of Immunonutrition and Microbiota Modulation

Interaction between Gut Microbiota and Dendritic Cells in Colorectal Cancer

Contact Info

Product

Resources

About