“…Three mat‐forming cyanobacterial strains previously isolated from Australian drinking water reservoirs (Gaget et al . ), namely I010‐003 ( Pseudanabaena galeata ), I010‐0123 ( Nostoc sp.) and I010‐0128 ( Anabaena sp., benthic form), were used to evaluate the consistency of DNA extraction methods for different species of Cyanobacteria.…”
These commercial kits allow greater recovery and the elimination of dangerous chemicals for DNA extraction, making them the method of choice for the isolation of DNA from benthic mats. They also facilitate the extraction of DNA from benthic Cyanobacteria, which can help to improve the characterization of Cyanobacteria in environmental studies using qPCRs or population composition analysis using next-generation sequencing.
“…Three mat‐forming cyanobacterial strains previously isolated from Australian drinking water reservoirs (Gaget et al . ), namely I010‐003 ( Pseudanabaena galeata ), I010‐0123 ( Nostoc sp.) and I010‐0128 ( Anabaena sp., benthic form), were used to evaluate the consistency of DNA extraction methods for different species of Cyanobacteria.…”
These commercial kits allow greater recovery and the elimination of dangerous chemicals for DNA extraction, making them the method of choice for the isolation of DNA from benthic mats. They also facilitate the extraction of DNA from benthic Cyanobacteria, which can help to improve the characterization of Cyanobacteria in environmental studies using qPCRs or population composition analysis using next-generation sequencing.
“…According to the numerical optimization analysis of the model, the DMSO concentration of 12.95%, the incubation time of 10.91 min and the cryopreservation duration of 111.44 days were determined as the optimum conditions which yielded the maximum cell viability with a desirability value of 0.97. Several studies showed higher viability using DMSO in a range of 5–15% for microalgal cryopreservation (Day et al ., 2005; Ernst et al ., 2005; Day, 2007; Gaget et al ., 2017). Fig.…”
Maintenance of eukaryotic microalgae strains for the long term is generally carried out using serial subculture techniques which require labour, time and cost. Cryopreservation techniques provide long-term storage of up to years for numerous microorganism strains and cell cultures. Ssu930ijn vbvbhnn8;l,n is related to a successfully designed mass and heat transfer balance throughout the cell. In this study, optimization of the cryopreservation process was carried out for two commercially used microalgal strains. The parameters to be optimized were DMSO percentage (0–25%), incubation time (1–15 min) and cryopreservation term (7–180 days) using a central composite design (CCD). Long-term storage up to 123.17 and 111.44 days corresponding to high cell viabilities was achieved for Chlorella vulgaris and Neochloris texensis, respectively. Generated models were found to be in good agreement with experimental results. The study also revealed holistic results for storage of microalgal strains in a stable state for industrial applications.
“…There are conflicting reports regarding the performance and choice of CPAs for the cryopreservation of photosynthetic organisms. Gaget et al (2017) used different concentrations of methanol, DMSO, and glycerol for the cryopreservation of 196 cyanobacterial isolates and found 5% DMSO to be the preferable choice of CPA for most of the strains [ 35 ]. In our study, 5% DMSO (except for the CB0101 strain), along with the EPS, also worked well for most of the strains.…”
Section: Discussionmentioning
confidence: 99%
“…The cryoprotective effect of EPS for the cryopreservation of cyanobacteria and microalgae was determined by a method used previously with some modifications [ 35 ]. Three different concentrations of EPS (10%, 15%, and 20%) from a stock solution (20 mg/mL) were used for cryopreservation.…”
Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of three unicellular cyanobacteria and two eukaryotic microalgae. Different concentrations of EPS (10%, 15%, and 20%) were tested, and the highest concentration (20%) of EPS yielded the best growth recovery for the algal strains we tested. We also compared EPS with 5% dimethyl sulfoxide (DMSO) and 10% glycerol for the cryopreservation recovery. The growth recovery for the microalgal strains after nine months of cryopreservation was better than 5% DMSO, a well-known cryoprotectant for microalgae. A poor recovery was recorded for all the tested strains with 10% glycerol as a cryoprotective agent. The patterns of growth recovery for most of these strains were similar after 5 days, 15 days, and 9 months of cryopreservation. Unlike common cryopreservants such as DMSO or methanol, which are hazardous materials, EPS is safe to handle. We demonstrate that the EPS from a psychrotrophic bacterium helped in the long-term cryopreservation of cyanobacteria and microalgae, and it has the potential to be used as natural cryoprotective agent for other cells.
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