From bacteria to biomedicine: Developing therapies exploiting NAD+ metabolism

Chen, Yu; Ying, Yuanyuan; Lalsiamthara, Jonathan; Zhao, Yuheng; Imani, Saber; Li, Xin; Liu, Sijing; Wang, Qingjing

doi:10.1016/j.bioorg.2023.106974

Cited by 9 publications

(1 citation statement)

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“…NAD+) would remove genetic alterations induced by metabolic auxotroph starvation. 27 Second, a strategy that strictly prevents bacterial growth outside of yeast independent of media. Third, further naturally selected (Tn IS) genomic minimization in the endosymbiont, and removal of the bacterial cell wall to reduce genetic alterations mediated by non-mitochondrial components of the bacterium.…”

Section: Discussionmentioning

confidence: 99%

Natural Selection in a Synthetic Yeast Endosymbiont Promotes Stable Coexistence

Supekova,

Zhou,

Barcellos

et al. 2024

Preprint

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Bacteria engulfment by a higher order host is believed to be the beginning of an evolutionary process that ultimately formed mitochondria. In an effort to experimentally elucidate the early effects of natural selection on bacteria resident in a eukaryotic host, a synthetic endosymbiont model system has been exploited. Here we describe a reproducible series of mutations that were observed afterEscherichia coliwas passaged withinSaccharomyces cerevisiaefor >8 passages which led to enhanced coexistence of bacteria within the yeast. These naturally selected mutations, formed by gene acquisition of trans-posable elements inrcsC, cpxA, andidnK, result in both functional and non-functional protein products with phenotypic effects on the bacteria that promote endosymbiont stability.

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Section: Discussionmentioning

confidence: 99%