From Batch to Continuous Manufacturing of Microbiomedical Devices

Madou, Marc; Florkey, J.

doi:10.1021/cr980098o

Cited by 42 publications

(22 citation statements)

References 28 publications

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“…The most common recent biosensor designs are typically fabricated using micromachining and/or silicon technology processes, such as vacuum evaporation, plasma etching, photolithography and lift-off. 27 Therefore, our method and results are likely to prove helpful for the design and development of a new generation of biosensors and bioassays.…”

Section: Discussionmentioning

confidence: 99%

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

2008

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Section: Discussionmentioning

confidence: 99%

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

2008

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“…The technique investigated by SatCon under this program substantially increases sensitivity through the use of single-mode planar waveguides and precision interferometry to detect changes [1][2][3][4][5][6] in the effective index of refraction resulting from surface binding. This approach differs from other evanescent techniques (e.g.…”

Section: -5mentioning

confidence: 99%

“…Place the H-filter on the test stand to initiate the run, and use a stopwatch to clock the run time. 4. At the end of the run, use a syringe to remove the product and the waste.…”

Section: -7mentioning

confidence: 99%

“…Su and Wong have reviewed several commercially available immunoassays developed to check for Staphylococcal enterotoxins with sensitivities ranging from 0.2 ng/ml to 1.0 ng/ml and a total assay time of 1.5 to 24 hours 4 . Some of the more rapid enterotoxin kits require special equipment or involve several handling steps common to most immunoassays.…”

Section: Introductionmentioning

confidence: 99%

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Phase 2 SBIR Final Report: An Ultra-Sensitive Optical Biosensor for Flood Safety

CAMBRIDGE¹

2002

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Subject Terms Report Classification unclassified Classification of this page unclassified Classification of Abstract unclassified Limitation of Abstract UU Number of Pages 247i Phase II SBIR Final Report: An Ultra-Sensitive Optical Biosensor for Food Safety (Contract DAAD16-01-C-0001) Executive Summary. The further development of a unique interferometric based optical biosensor platform for the rapid unlabelled detection and identification of foodborne pathogens was carried out under Phase II SBIR contract DAAD16-01-C-0001 entitled: An Ultra-Sensitive Optical Biosensor for Food Safety. The potential in combining optical methods with biomolecular recognition techniques is the high signal gain derived from photonic amplification and thus high sensitivity, specificity, and rapid response time.This Phase II effort was focused on the development of the following innovations:• Microfluidic diffusional filtering of bacterial cells for high-throughput 'on-chip' purification of real-world samples prior to detection, • Biomolecular immobilization based on the application of thin self-assembled polyethylene oxide derived molecular films with for highly controlled positioning of receptor sites and the suppression of non-specific binding events, • Uigh resolution interferometric evanescent wave detection based on the modulation of polarization and the subsequent differencing of TE and TM modes for highly stable and self-compensated operation, and • The spatial manipulation of the cells within a microfluidic environment to steer the sample stream nearer to the receptors for increased probability of capture.The goal of the effort was the eventual development of a portable biosensing platform for the detection and identification of viable pathogenic microbes in foods at the level of 1 CFU/mL within a 30-minute period.The usual 24-month Phase II SBIR program was accelerated to hasten the commercialization of the technology, however the program was terminated at 18 months after both contract funding and SatCon cost-share had been expended, and no follow-on funding was attainable. The original proposal, written only four months into the Phase I effort, was modified to perform a bench-top level effort and not the fully miniaturized development originally proposed. Costsharing equivalent to the indirect program costs was borne by SatCon.Laboratory Development. The different sub-systems were developed, tested, and integrated to the extent possible. A summary of these efforts are provided below:• A 'doubly differential' interferometer, relying on the difference in evanescence response between modulating polarizations, was built. The transducer monitored changes in the speed of light caused by surface binding propagating through a single-mode waveguide. A first generation grating coupled waveguide was fabricated but was unusable due to poor light throughput. The design of the next generation waveguide was improved with the incorporation of 1 st order diffraction gratings. A commercial fabrication facility capable of doing the ...

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“…[1][2] Especially, disposable polymer microdevices have been adapted in many fluidics-based applications because of the low fabrication cost and the easiness of complex shape fabrication. [3][4] Most of these polymer devices still consist of simple channels, valves, and chambers mainly, though polymer devices have been miniaturized for rapid and parallel processing. For further miniaturization and practical usage, many important functions such as heating and detection should be implemented with the polymer components.…”

Section: Introductionmentioning

confidence: 99%

Plastic thermally controllable platform with integrated thin film microcomponents

et al. 2005

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We present a novel technology for a cyclo-olefin-copolymer (COC) plastic microfluidic platform for heat control with fully semiconductor process-compatible photolithographic 5 m-wide metal patterns, for heaters, electrodes, and temperature sensors and a thin membrane structure.Through tests of compatibility of some thermoplastic materials with chemical solutions and temperature tolerance to the semiconductor processes (thin film depositions, photolithography, and etchings), we selected COC as a semiconductor process-compatible plastic material for biomedical applications. For photolithography processes, we manufactured the 5' COC wafer with flat surface with c.a. 3 nm surface roughness, employing a novel flame-torched injection-molding method. Furthermore, the part of heating blocks on COC wafers is controlled thickness to the 100 m, to enhance the heat-ramping speeds through reduction of the thermal mass. In order to fabricate the Au thin film micro-patterns for temperature sensors, heaters, and electrodes, Au film (100 nm) was deposited by e-beam evaporator and patterned by using standard photolithography, and wet-etched. The micropatterned Au temperature sensors, heaters, and electrodes was demonstrated For insulating layers, Al 2 O 3 film was deposited by an ALD system, patterned by using the standard photolithography, and wet-etched.Using the COC microfluidic platform, we tested thermal cycling with simple heating and natural cooling on chip with water and, heating rates (5 /s when heating, 3 /s when cooling) are obtained. Therefore, the COC microfluidic platform can be applied to a DNA lab-on-a-chip.

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From Batch to Continuous Manufacturing of Microbiomedical Devices

Cited by 42 publications

References 28 publications

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

Amperometric Biosensor Based on Glucose Dehydrogenase and Plasma-polymerized Thin Films

Phase 2 SBIR Final Report: An Ultra-Sensitive Optical Biosensor for Flood Safety

Plastic thermally controllable platform with integrated thin film microcomponents

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