From cloned frogs to patient matched stem cells: induced pluripotency or somatic cell nuclear transfer?

Yamada, Mitsutoshi; Byrne, James A.; Egli, Dieter

doi:10.1016/j.gde.2015.06.007

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…However, the functionality of reprogrammed human stem cells has not been sufficiently tested. Notably, nuclear transfer (NT) from adult cells more consistently results in the production of viable mice (6) than in the production from induced pluripotent stem cells (iPSCs) (7), suggesting that reprogrammed cells derived by SCNT are more often fully differentiation competent (8). Reprogramming by NT recapitulates developmental events that occur upon normal fertilization and allows resetting of the epigenome of the somatic nucleus to an early embryonic state.…”

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confidence: 99%

β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells

et al. 2017

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β-Cells derived from stem cells hold great promise for cell replacement therapy for diabetes. Here we examine the ability of nuclear transfer embryonic stem cells (NT-ESs) derived from a patient with type 1 diabetes to differentiate into β-cells and provide a source of autologous islets for cell replacement. NT-ESs differentiate in vitro with an average efficiency of 55% into C-peptide-positive cells, expressing markers of mature β-cells, including MAFA and NKX6.1. Upon transplantation in immunodeficient mice, grafted cells form vascularized islet-like structures containing MAFA/C-peptide-positive cells. These β-cells adapt insulin secretion to ambient metabolite status and show normal insulin processing. Importantly, NT-ES-β-cells maintain normal blood glucose levels after ablation of the mouse endogenous β-cells. Cystic structures, but no teratomas, were observed in NT-ES-β-cell grafts. Isogenic induced pluripotent stem cell lines showed greater variability in β-cell differentiation. Even though different methods of somatic cell reprogramming result in stem cell lines that are molecularly indistinguishable, full differentiation competence is more common in ES cell lines than in induced pluripotent stem cell lines. These results demonstrate the suitability of NT-ES-β-cells for cell replacement for type 1 diabetes and provide proof of principle for therapeutic cloning combined with cell therapy.

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confidence: 99%

β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells

et al. 2017

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“…It is our opinion that SCNT remains a unique tool that can radically reprogram the genome and does so very rapidly. These latest findings herald good news, both for therapeutic cloning [41] and for deriving iPSCs. SCNT, particularly in its variant interspecies somatic cell nuclear transfer (iSCNT) [42][43][44], where the somatic cell and the oocyte come from different species, might provide insights into the very early totipotent genes.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 98%

A New, Dynamic Era for Somatic Cell Nuclear Transfer?

Loi

Iuso

Czernik

et al. 2016

Trends in Biotechnology

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“…We first generated cloned mice using adult brain neurons from the hippocampus and cerebral cortex [ 50 ]. These cells are known to be unreprogrammable cells for iPS cell generation, which indicates that genomic reprogramming using enucleated oocytes has a greater potential than the forced expression of reprogramming transcription factors [ 84 ].…”

Section: Nuclear Transfermentioning

confidence: 99%

Development of reproductive engineering techniques at the RIKEN BioResource Center

Ogura

2017

Exp. Anim.

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Reproductive engineering techniques are essential for assisted reproduction of animals and generation of genetically modified animals. They may also provide invaluable research models for understanding the mechanisms involved in the developmental and reproductive processes. At the RIKEN BioResource Center (BRC), I have sought to develop new reproductive engineering techniques, especially those related to cryopreservation, microinsemination (sperm injection), nuclear transfer, and generation of new stem cell lines and animals, hoping that they will support the present and future projects at BRC. I also want to combine our techniques with genetic and biochemical analyses to solve important biological questions. We expect that this strategy makes our research more unique and refined by providing deeper insights into the mechanisms that govern the reproductive and developmental systems in mammals. To make this strategy more effective, it is critical to work with experts in different scientific fields. I have enjoyed collaborations with about 100 world-recognized laboratories, and all our collaborations have been successful and fruitful. This review summarizes development of reproductive engineering techniques at BRC during these 15 years.

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From cloned frogs to patient matched stem cells: induced pluripotency or somatic cell nuclear transfer?

Cited by 6 publications

References 34 publications

β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells

β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells

A New, Dynamic Era for Somatic Cell Nuclear Transfer?

Development of reproductive engineering techniques at the RIKEN BioResource Center

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