From DNA barcoding to personalized nutrition: the evolution of food traceability

Galimberti, Andrea; Galimberti, Andrea; Bruni, Ilaria; Guzzetti, Lorenzo; Cortis, Pierluigi; Berterame, Nadia Maria; Labra, Massimo

doi:10.1016/j.cofs.2019.07.008

Cited by 45 publications

(32 citation statements)

References 50 publications

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“…A revolutionary step forward in promoting greater traceability and transparency was made with the advent of DNA barcoding, a molecular technique based on the DNA-level identification of differences that univocally characterize individual species 15 . Since the launch of the DNA barcoding initiative 16 , more than 500 peer-reviewed documents pertaining to DNA barcoding applied to food traceability have been published 17 . The spread of next-generation sequencing (NGS) platforms has further improved the potential of this technique, speeding up the possibility of simultaneously analysing multiple ingredients from complex matrixes 18 .…”

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confidence: 99%

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

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Scariolo

Vannozzi

et al. 2020

Sci Rep

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Pet food industry has grown considerably in the last few years and it is expected to continue with this rate. Despite the economic impact of this sector and the consumer concerns for the increasing number of food and feed adulteration cases, few studies have been published on mislabelling in pet foods. We therefore investigated the capability of a next generation sequencing-based mini-barcoding approach to identify animal species in pet food products. In a preliminary analysis, a 127 bp fragment of the COI gene was tested on both individual specimens and ad hoc mixed fresh samples used as testers, to evaluate its discrimination power and primers effectiveness. Eighteen pet food products of different price categories and forms available on the market (i.e. kibbles, bites, pâté and strips) were analysed through an NGS approach in biological replicates. At least one of the species listed in the ingredients was not detected in half of the products, while seven products showed supplementary species in addition to those stated on the label. Due to the accuracy, sensitivity and specificity demonstrated, this method can be proposed as food genetic traceability system to evaluate both the feed and food quality timely along the supply chain.

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confidence: 99%

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

Palumbo

Scariolo

Vannozzi

et al. 2020

Sci Rep

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“…The identification of a microbial signature for traceability purposes was suggested also by forensic scientists as natural consequence of the application of HTS technologies in a wider perspective (Bishop, 2019): with the globalisation of trade, food traceability is a hot topic and identifying a microbial signature in these products can provide a deeper insight into the "food ecosystem" (Galimberti et al, 2015;Bokulich, Lewis, Boundy-Mills, Mills, 2016;Galimberti et al, 2019;Parente et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Tasting the differences: microbiota analysis of different insect-based novel food

Frigerio

Agostinetto

Galimberti

et al. 2020

Preprint

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Traceability, quality and safety of edible insects are important both for the producers and the consumers. Today, alongside the burst of edible insects in western countries, we are facing a gap of knowledge of insect microbiota associated with the microbial ecosystems of insectbased products. Recent studies suggest that the insect microbiota can vary between insect species and that can be shaped by additional factors, such as rearing conditions. Also, the production processes of raw materials (i.e. insect flour) into final food products can affect the insect microbiota too. This has consequences for the evaluation of food safety and food traceability. In this context, High-Throughput DNA Sequencing (HTS) techniques can give insight into the carryover of insect microbiota into final food products. In this study, we investigated the microbiota composition of insect-based commercial food products, applying 2 HTS techniques coupled with bioinformatic analysis. The aim of this work was to analyse the microbiota variability of different categories of insect-based products made of A. domesticus (house cricket), T. molitor (mealworm beetle), and A. diaperinus (lesser mealworm or litter beetle), including commercial raw materials and processed food items, purchased via ecommerce from different companies. Our data revealed that samples cluster per insect species based on microbiota profile and preliminary results suggested that a small number of prevalent bacteria formed a "core microbiota" characterizing the products depending on the insect, suggesting that a resident microbiota is conserved. This microbial signature can be recognized despite the different food processing levels, rearing conditions selling companies.We showed that differences exist when comparing raw vs processed food made of the same insect, or similar products produced by different companies as well, laying the groundwork for further analyses. These results support the application of HTS analysis for studying the composition of processed insect food in a wider perspective, for food traceability and food quality control.

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“…A different scenario occurs for the trading of food commodities for which strict regulations about quality and safety impose the adoption of analytical tools and innovation technologies to prevent the spread of foodborne pathogens or other contaminants ( 12 , 13 ). In most cases, food quality evaluation is based on bioindicators, both at chemical and microbiological level ( 14 , 15 ).…”

Section: Pandemics Preventionmentioning

confidence: 99%

Rethinking Urban and Food Policies to Improve Citizens Safety After COVID-19 Pandemic

et al. 2020

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The ongoing pandemic caused by the coronavirus disease 2019 (COVID-19) is literally changing the world. From December 2019 to date, more than 22 million cases have been reported worldwide and global health institutions are acting to slow down the virus transmission and are looking for possible prevention strategies in case of a new outbreak. As in other endemic or pandemic phenomena, the issues mostly covered by scientific and media attention are related to the diagnostic and therapeutic approach of COVID-19. However, a still neglected issue regards the adoption of a more systemic approach considering the close connection among the infection, the environment, and human behaviors, including the role of diet and urban management. To shed light on this issue, we brought together a faculty group involving experts in environment and biodiversity, food safety, human nutrition, and behavior, bioprospecting, as well as medical doctors having a deep knowledge of the complex historical relationship between humanity and vector-borne infections. Two main aspects emerged from the integrative overview of the current COVID-19 pandemic: (i) the scientific community should start sharing social actions and policy advocacy based on the assumption that human health strongly depends upon a sustainable exploitation of natural resources in populated areas; (ii) the specific strategic role of the cities in developing sustainable food systems and promoting healthy dietary patterns. Definitely, some priority issues should be addressed to achieve these goals, such as global efforts to increase food safety and security, which would benefit from urban and peri-urban agriculture enhancement, smallholder food producers support, and ecosystem services and local biodiversity maintenance.

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From DNA barcoding to personalized nutrition: the evolution of food traceability

Cited by 45 publications

References 50 publications

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

NGS-based barcoding with mini-COI gene target is useful for pet food market surveys aimed at mislabelling detection

Tasting the differences: microbiota analysis of different insect-based novel food

Rethinking Urban and Food Policies to Improve Citizens Safety After COVID-19 Pandemic

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