From fin rays to DNA: supplementary morphological and molecular data to identify Mormyrus subundulatus Roberts, 1989 (Pisces: Mormyridae) from the Bandama River in Côte d'Ivoire

Prié, Vincent; Adam, Benjamin; Melki, Frédéric

doi:10.5252/zoosystema2021v43a27

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…To assess the phylogenetic relationships for taxa sequenced in this study, 57 additional COI sequences were retrieved from GenBank. This included five outgroup taxa, GenBank sequences of C. citharinus HQ927822.1 [47], Pristolepis fasciata [48], Acanthocleithron chapini KT192951.1 [49], Hepsetus odoe HM882992.1 [50], Bagrus bajad MK335908.1 [51] and Marcusenius senegalensis HM882735.1 [52] were employed as outgroups for cichlids, channids, mochokids, alestids, claroteids and mormyrids for the analyses, respectively. The rest of the sequences represented six families: Cichlidae, Channidae, Mochokidae, Alestidae, Claroteidae and Mormyridae (S2 Table ).…”

Section: Phylogenetic Analysis and Sequence Divergencementioning

confidence: 99%

DNA barcoding affirms the presence of invasiveParachanna obscuraand new records of some species in the Mweru-Luapula fishery

Seemani,

Katongo,

Bloomer

et al. 2024

Preprint

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DNA barcoding has recently been instrumental in identifying both invasive and undetected species in aquatic environments. This study aimed at analysing collected fish fin clips to ascertain the identity of native species and the Parachanna species that have invaded the Mweru-Luapula (ML) fishery of Zambia. The identification process was carried out through field phenotypic analysis using species guides and DNA barcoding, with the mitochondrial DNA (mtDNA) cytochrome C oxidase 1 (COI) gene fragment. Of the 28 specimens for which DNA was successfully PCR amplified, five matched the reference sequences of species and 22 matched the reference sequences of genera on the NCBI GenBank. Five unexpected species, namely Oreochromis niloticus, Coptodon zillii, Mormyrus kannume, Thoracochromis buysi and Tylochromis polylepis were identified. The study further affirmed the presence of invasive Parachanna obscura in the fishery and its interconnected water bodies. Parachanna obscura invaded the fishery through annual flooding from aquacultural facilities in the Democratic Republic of Congo (DRC). There is a need to investigate this invasion further by using large sample sizes and also by applying the gonadosomatic index (GSI) to determine invasive species occupancy and impact on native species throughout the fishery. This study provides a platform for further detailed taxonomic verification and species inventory of the entire ML fishery. This will facilitate the development of a viable and sustainable strategy to appropriately curb the impact of invasive species, and will thus contribute to the conservation of the ML aquatic biodiversity.

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