From glass to life: a commentary on the assessment of the reproductive potential of cryopreserved human oocytes

Plancha, Carlos E.; Kovačič, Borut

doi:10.1007/s10815-022-02565-2

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Oocyte cryopreservation is a novel method in assisted reproductive technologies that aims at overcoming technical difficulties. The potential of a cryopreserved oocyte to lead to a live birth was calculated as 5% to 4.3%, although confirmation from larger samples seems necessary [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Live Birth from Cryopreserved Oocyte After Uterus Transplantation: A Case Report

Scollo,

Scibilia,

Vento

et al. 2023

Am J Case Rep

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Patient: Female, 28-year-old Final Diagnosis: Mayer-Rokitansky-Küster-Hauser syndrome Symptoms: Amenorrhea Clinical Procedure: Uterus transplant Specialty: Obstetrics and Gynecology • Transplantology Objective: Rare disease Background: Important legal and ethical issues must be addressed in the practice of uterus transplantation, because it is a non-life-saving intervention. In all cases reported in the literature so far, uterus transplantation is preceded by oocyte retrieval, fertilization of the collected oocytes, and subsequent freezing of the embryos produced. This element should be considered because of the potential ethical, legal, and moral implications related to the existence and fate of supernumerary embryos in the event of transplantation failure. Case Report: The Italian Research Project for Uterus Transplantation from a brain-dead donor was approved in 2018 (No. 1438/CNT2018). A 28-year-old patient with Mayer-Rokitansky-Küster-Hauser syndrome, ectopic ovaries, and good ovarian reserve received uterus transplantation in 2020 after oocyte retrieval with laparoscopic assistance. Metaphase oocytes were cryopreserved and thawed after the successful transplantation to perform in vitro fertilization followed by embryo transfer. The pregnancy course was regular, without symptoms until week 30, when PCR positivity for SARS-CoV-2 was recorded. The patient underwent an emergency cesarean delivery at 34 weeks’ gestation because of fever and the appearance of regular uterine contractions. An infant was born alive and vital at 34 weeks of pregnancy and weighed 1725 g. The newborn was discharged in good condition and with a body weight of 2740 g. Conclusions: This case report shows that cryopreservation of oocytes can overcome the ethical issue related to embryo retrieval before a successful uterus transplantation can be demonstrated. Our result supports the possibility of bypassing embryo freezing before ascertaining the success of uterus transplantation.

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Section: Discussionmentioning

confidence: 99%

Live Birth from Cryopreserved Oocyte After Uterus Transplantation: A Case Report

Scollo,

Scibilia,

Vento

et al. 2023

Am J Case Rep

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Timing of ICSI with Respect to Meiotic Spindle Status

Teplá

Topurko

Jirsová

et al. 2022

IJMS

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The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5–6 h after ovum pick-up (OPU) or 7–8 h after OPU. Oocytes without MS evaluation were all fertilized 5–6 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58/182); while 195 patients without MS visualization had 24% PR (47/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58/140), while 162 patients without MS visualization had 41% PR (66/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.

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From glass to life: a commentary on the assessment of the reproductive potential of cryopreserved human oocytes

Cited by 2 publications

References 12 publications

Live Birth from Cryopreserved Oocyte After Uterus Transplantation: A Case Report

Live Birth from Cryopreserved Oocyte After Uterus Transplantation: A Case Report

Timing of ICSI with Respect to Meiotic Spindle Status

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