From Membrane Pores to Aquaporins: 50 Years Measuring Water Fluxes

Parisi, Mario; Dorr, Ricardo; Ozu, Marcelo; Toriano, Roxana

doi:10.1007/s10867-008-9064-5

Cited by 27 publications

(22 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it would be hard to explain water permeability in cells, such as erythrocytes, renal tubular epithelial cells or gametes, if water only passed through plasma membrane via simple diffusion (Huang et al., ). For this reason, Sidel and Solomon () early assumed that water should mainly flow through cell membranes by a passive transport mechanism other than simple diffusion (Parisi, Dorr, Ozu, & Toriano, ).…”

Section: Introductionmentioning

confidence: 99%

Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

Yeste

Morató

Rodríguez-Gil

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents Aquaporins (AQPs) play a vital role for the transport of water and solutes across cell membranes. Classification of these ubiquitous proteins into three categories (orthodox AQPs, aquaglyceroporins and superaquaporins) is based on their sequence similarity and substrate selectivity. In the male reproductive tract of mammals, most AQPs (except AQP6 and AQP12) are found in different organs (including testis, efferent ducts and epididymis). AQP1 and AQP9 are the most abundant AQPs in the efferent ducts and epididymis and play a crucial role for the secretion/reabsorption dynamics of luminal fluid during sperm transport and maturation. AQP3, AQP7, AQP8 and AQP11 are the most abundant AQPs in sperm and are involved in the regulation of their volume, which is required for the differentiation of spermatids into spermatozoa during spermatogenesis, as well as in sperm transit along environments of different osmolality (male and female reproductive tracts). While different studies conducted in oocytes and embryos have demonstrated that AQPs are important for cryotolerance, data in sperm are scarce. At present, mounting evidence indicates that AQP3, AQP7 and AQP11 are involved in the sperm response to variations of osmolality and to freeze‐thawing procedures. All these studies contribute to understand the physiology of both male reproductive tract and sperm, and open up new research ventures on the improvement of sperm cryopreservation protocols.

show abstract

Section: Introductionmentioning

confidence: 99%

Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

Yeste

Morató

Rodríguez-Gil

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Traditionally, P f is calculated by measuring changes in the volume of whole oocytes a few seconds after decreasing the osmolality of the extracellular medium (11,29). This method has proved convenient for identifying aquaporins and for studying their biophysical properties (24). The emptied-out oocyte technique developed in our laboratory offers the advantage of being able to control both intracellular and extracellular medium compositions, which broadens experimental possibilities.…”

Section: Resultsmentioning

confidence: 99%

“…Several articles and reviews of research in transmembrane water transport have been published (4,14,24,25). To facilitate the comprehension of this article, a brief reference framework is offered to the readers.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A counterpoint between computer simulations and biological experiments to train new members of a laboratory of physiological sciences

Ozu

Dorr

Gutiérrez

et al. 2012

Advances in Physiology Education

Self Cite

View full text Add to dashboard Cite

When new members join a working group dedicated to scientific research, several changes occur in the group's dynamics. From a teaching point of view, a subsequent challenge is to develop innovative strategies to train new staff members in creative thinking, which is the most complex and abstract skill in the cognitive domain according to Bloom's revised taxonomy. In this sense, current technological and digital advances offer new possibilities in the field of education. Computer simulation and biological experiments can be used together as a combined tool for teaching and learning sometimes complex physiological and biophysical concepts. Moreover, creativity can be thought of as a social process that relies on interactions among staff members. In this regard, the acquisition of cognitive abilities coexists with the attainment of other skills from psychomotor and affective domains. Such dynamism in teaching and learning stimulates teamwork and encourages the integration of members of the working group. A practical example, based on the teaching of biophysical subjects such as osmosis, solute transport, and membrane permeability, which are crucial in understanding the physiological concept of homeostasis, is presented.

show abstract

“…In principle, to account for net water transport through the membrane, one should consider not only Jv , as defined in Equation 1b, but also the contribution of solvent drag by net solute transport. However, the latter can be considered negligible, because in most animals the majority of solvent flow is via aquaporin channels that are quite selective for water (Parisi et al, 2007). …”

Section: Modeling Approaches To Understanding Hypotonic Cell Volume Rmentioning

confidence: 99%

Regulatory volume decrease and P receptor signaling in fish cells: mechanisms, physiology, and modeling approaches

et al. 2011

View full text Add to dashboard Cite

For animal cell plasma membranes, the permeability of water is much higher than that of ions and other solutes, and exposure to hyposmotic conditions almost invariably causes rapid water influx and cell swelling. In this situation, cells deploy regulatory mechanisms to preserve membrane integrity and avoid lysis. The phenomenon of regulatory volume decrease, the partial or full restoration of cell volume following cell swelling, is well-studied in mammals, with uncountable investigations yielding details on the signaling network and the effector mechanisms involved in the process. In comparison, cells from other vertebrates and from invertebrates received little attention, despite of the fact that e.g. fish cells could present rewarding model systems given the diversity in ecology and lifestyle of this animal group that may be reflected by an equal diversity of physiological adaptive mechanisms, including those related to cell volume regulation. In this review, we therefore present an overview on the most relevant aspects known on hypotonic volume regulation presently known in fish, summarizing transporters and signaling pathways described so far, and then focus on an aspect we have particularly studied over the past years using fish cell models, i.e. the role of extracellular nucleotides in mediating cell volume recovery of swollen cells. We, furthermore, present diverse modeling approaches developed on the basis of data derived from studies with fish and other models and discuss their potential use for gaining insight into the theoretical framework of volume regulation.

show abstract

From Membrane Pores to Aquaporins: 50 Years Measuring Water Fluxes

Cited by 27 publications

References 102 publications

Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

Aquaporins in the male reproductive tract and sperm: Functional implications and cryobiology

A counterpoint between computer simulations and biological experiments to train new members of a laboratory of physiological sciences

Regulatory volume decrease and P receptor signaling in fish cells: mechanisms, physiology, and modeling approaches

Contact Info

Product

Resources

About