From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering

Baudry, Anne; Uzunoğlu, Emel; Schneider, Benoı̂t; Kellermann, Odile; Goldberg, Michel

doi:10.1186/s41121-016-0002-x

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…It is reported that it is easier for cells to perform amoeboid movement through fibrous scaffolds, giving higher proliferation rates. [43][44][45][46][47] It is noteworthy that in these cases, the aspect of discussion of chemical effects on the cells and thereby their biological activity remains untouched. Therefore, elutes were prepared for the indirect contact test, as recommended by the ISO.…”

Section: Discussionmentioning

confidence: 99%

Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

et al. 2018

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The aim was to develop an economical and biocompatible collagen-based bioactive composite for tooth regeneration. Acid-soluble collagen was extracted and purified from fish scales. The design was innovated to molecularly tailor the surface charge sites of the nano-apatite providing chemical bonds with the collagen matrix via microwave irradiation technique. The obtained collagen was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The composites were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis/differential scanning calorimetry, and scanning electron microscopy. MC3T3-E1 cell lines were used to assess the biological effects of these materials by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetra zolium bromide (MTT) assay. Indirect contact test was performed by extracting representative elutes in cell culture media and sulforhodamine B analysis was performed. Chorioallantoic membrane assay was conducted to define the new vessels formation behavior. The purity of collagen extracts was determined and showed two α-chains, i.e. the characteristic of type I collagen. Fourier transform infrared spectroscopy showed the characteristic peaks for amide I, I, III, and phosphate for collagen and composites. Scanning electron microscopy images showed three-dimensional mesh of collagen/apatite nano-fibers. Nontoxic behavior of composites was observed and there were graded and dose-related effects on experimental compounds. The angiogenesis and vessels formation behavior were observed in bioactive collagen composite. The obtained composites have potential to be used for tooth structure regeneration.

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Section: Discussionmentioning

confidence: 99%

Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

et al. 2018

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“…SCAPs, derived from the human apical papilla of growing tooth roots, 50 are a significant population of MSCs in endodontics regeneration 51 . The SCAP cultures can differentiate into odontoblast‐like cells with potential proliferation and mineralization 52 .…”

Section: Dental Stem Cells In Dentin Regenerationmentioning

confidence: 99%

Dentin regeneration based on tooth tissue engineering: A review

Azaryan

Razavi

Hanafi‐Bojd

et al. 2023

Biotechnology Progress

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Missing or damaged teeth due to caries, genetic disorders, oral cancer, or infection may contribute to physical and mental impairment that reduces the quality of life.Despite major progress in dental tissue repair and those replacing missing teeth with prostheses, clinical treatments are not yet entirely satisfactory, as they do not regenerate tissues with natural teeth features. Therefore, much of the focus has centered on tissue engineering (TE) based on dental stem/progenitor cells to create bioengineered dental tissues. Many in vitro and in vivo studies have shown the use of cells in regenerating sections of a tooth or a whole tooth. Tooth tissue engineering (TTE), as a promising method for dental tissue regeneration, can form durable biological substitutes for soft and mineralized dental tissues. The cell-based TE approach, which directly seeds cells and bioactive components onto the biodegradable scaffolds, is currently the most potential method. Three essential components of this strategy are cells, scaffolds, and growth factors (GFs). This study investigates dentin regeneration after an injury such as caries using TE and stem/progenitor cell-based strategies. We begin by discussing about the biological structure of a dentin and dentinogenesis.The engineering of teeth requires knowledge of the processes that underlie the growth of an organ or tissue. Then, the three fundamental requirements for dentin regeneration, namely cell sources, GFs, and scaffolds are covered in the current study, which may ultimately lead to new insights in this field.

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“…Endodontic therapy is fraught with challenges, such as complexity and variability in root canal anatomy, microbial challenges, handling complications, and addressing patient-related factors that significantly impact treatment outcomes and necessitate careful consideration and expertise. Recently, stem-cell-based regeneration of odontogenic tissues has gained popularity due to its non-invasive nature [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bacterial Metabolites on the Wnt4 Protein in Dental-Pulp-Stem-Cells-Based Endodontic Pulpitis Treatment

et al. 2023

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Porphyromonas gingivalis is associated with endodontic pulpitis, causing damage to the dental pulp, leading to severe pain and a decline in quality of life. Regenerative pulp treatments using dental pulp stem cells (DPSCs) can be hindered by interactions between DPSCs and the infecting bacteria. The protein WNT family member 4 (Wnt4) plays a critical role in the differentiation of DPSCs and the regeneration of odontogenic tissue. However, the specific influence of P. gingivalis on Wnt4 remains unclear. In this study, we employed a computational approach to investigate the underlying mechanisms through which P. gingivalis-produced metabolites inhibit the Wnt4 protein, thereby diminishing the regenerative potential and therapeutic efficacy of odontogenic tissue. Among the metabolites examined, C29H46N7O18P3S−4 exhibited the strongest inhibitory effect on the Wnt4 protein, as evidenced by the lowest binding energy score of −6782 kcal/mol. Molecular dynamic simulation trajectories revealed that the binding of C29H46N7O18P3S−4 significantly altered the structural dynamics and stability of the Wnt4 protein. These alterations in protein trajectories may have implications for the molecular function of Wnt4 and its associated pathways. Overall, our findings shed light on the inhibitory impact of P. gingivalis-produced metabolites on the Wnt4 protein. Further in vitro, in vivo, and clinical studies are necessary to validate and expand upon our findings.

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From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering

Cited by 6 publications

References 29 publications

Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

Dentin regeneration based on tooth tissue engineering: A review

Effects of Bacterial Metabolites on the Wnt4 Protein in Dental-Pulp-Stem-Cells-Based Endodontic Pulpitis Treatment

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