Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

Abstract

The aim was to develop an economical and biocompatible collagen-based bioactive composite for tooth regeneration. Acid-soluble collagen was extracted and purified from fish scales. The design was innovated to molecularly tailor the surface charge sites of the nano-apatite providing chemical bonds with the collagen matrix via microwave irradiation technique. The obtained collagen was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The composites were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis/differential scanning calorimetry, and scanning electron microscopy. MC3T3-E1 cell lines were used to assess the biological effects of these materials by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetra zolium bromide (MTT) assay. Indirect contact test was performed by extracting representative elutes in cell culture media and sulforhodamine B analysis was performed. Chorioallantoic membrane assay was conducted to define the new vessels formation behavior. The purity of collagen extracts was determined and showed two α-chains, i.e. the characteristic of type I collagen. Fourier transform infrared spectroscopy showed the characteristic peaks for amide I, I, III, and phosphate for collagen and composites. Scanning electron microscopy images showed three-dimensional mesh of collagen/apatite nano-fibers. Nontoxic behavior of composites was observed and there were graded and dose-related effects on experimental compounds. The angiogenesis and vessels formation behavior were observed in bioactive collagen composite. The obtained composites have potential to be used for tooth structure regeneration.

Preparation and Evaluation of Peptides with Potential Antioxidant Activity by Microwave Assisted Enzymatic Hydrolysis of Collagen from Sea Cucumber Acaudina Molpadioides Obtained from Zhejiang Province in China

“…However, the degree of hydrolysis of collagen is incomplete, and the molecular distribution of hydrolysate is uneven in the traditional methods. Microwave digestion is an effective method for extracting and hydrolyzing collagen [28,29]. The energy of microwaves can penetrate to the interior of collagen molecule and prevent its aggregation, resulting in complete hydrolysis.…”

Ekstraksi Kolagen dari Ikan Tuna Sirip Kuning dengan Bantuan Microwave

“…Daerah puncak amida III dari penelitian sebelumnya muncul di 1240 cm -1 , sedangkan pada kolagen ini puncak berada di 1269 cm -1 . Semakin tinggi panjang gelombang yang diserap memperlihatkan ikatan silang inter-molecular yang terbuka yang menyebabkan hilangnya susunan molecular yang sebelumnya ada [13].…”

Ionizing Radiation for Preparation and Functionalization of Membranes and Their Biomedical and Environmental Applications

“…A number of technical approaches have been applied for scaffold production. In these cases, scaffolds should not only be a substitute of the extracellular matrix but also act as a membrane able to act as a delivery vehicle for cells and/or to serve as a carrier for growth factors [5,[66][67][68][69][70][71][72]. The final focus is the same: to engineer membranes with adequate properties able to improve interactions with the cells and to enhance tissue regeneration.…”